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Description
Rat INSL3 (Insulin-like 3) ELISA KitThe Rat (INSL3) Insulin like 3 ELISA Kit measures Insulin like 3 in Rat samples. The plate has been pre coated with Rat INSL3 antibody. INSL3 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Rat INSL3 Antibody is added and binds to INSL3 in the sample. Then Streptavidin HRP is added and binds to the Biotinylated INSL3 antibody. After incubation unbound Streptavidin HRP is washed away during a washing
The Rat (INSL3) Insulin-like 3 ELISA Kit measures Insulin-like 3 in Rat samples. The plate has been pre-coated with Rat INSL3 antibody. INSL3 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Rat INSL3 Antibody is added and binds to INSL3 in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated INSL3 antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Rat INSL3. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.
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