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Description
Rat NT-4 (Neurotrophin 4) ELISA KitThe Rat (NT 4) Neurotrophin 4 ELISA Kit measures Neurotrophin 4 in Rat samples. The plate has been pre coated with Rat NT 4 antibody. NT 4 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Rat NT 4 Antibody is added and binds to NT 4 in the sample. Then Streptavidin HRP is added and binds to the Biotinylated NT 4 antibody. After incubation unbound Streptavidin HRP is washed away during a washing step.
The Rat (NT-4) Neurotrophin 4 ELISA Kit measures Neurotrophin 4 in Rat samples. The plate has been pre-coated with Rat NT-4 antibody. NT-4 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Rat NT-4 Antibody is added and binds to NT-4 in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated NT-4 antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Rat NT-4. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.
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