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Description
Human SPARCL1 (Sparc-like Protein 1) ELISA KitThe Human (SPARCL1) Sparc like Protein 1 ELISA Kit measures SPARC like protein 1 in samples. The plate has been pre coated with Human SPARCL1 antibody. SPARCL1 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Human SPARCL1 Antibody is added and binds to SPARCL1 in the sample. Then Streptavidin HRP is added and binds to the Biotinylated SPARCL1 antibody. After incubation unbound Streptavidin HRP is
The Human (SPARCL1) Sparc-like Protein 1 ELISA Kit measures SPARC-like protein 1 in samples. The plate has been pre-coated with Human SPARCL1 antibody. SPARCL1 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Human SPARCL1 Antibody is added and binds to SPARCL1 in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated SPARCL1 antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Human SPARCL1. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.
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