Mouse MHC-III/H-2III (Major Histocompatibility Complex-III) ELISA Kit

Mouse MHC-III/H-2III (Major Histocompatibility Complex-III) ELISA KitThe Mouse MHC III H 2III (Major Histocompatibility Complex III) ELISA immunoassay kit uses the Sandwich ELISA principle. The micro ELISA plate provided in this kit has been pre coated with an antibody specific to Mouse MHC III H 2III. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse MHC III H 2III and Avidin Horseradish Peroxidase (HRP)

The Mouse MHC-III/H-2III (Major Histocompatibility Complex-III) ELISA immunoassay kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse MHC-III/H-2III. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse MHC-III/H-2III and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse MHC-III/H-2III, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Mouse MHC-III/H-2III. You can calculate the concentration of Mouse MHC-III/H-2III in the samples by comparing the OD of the samples to the standard curve.