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Description
PGE2 (Prostaglandin E2) ELISA KitThe Prostaglandin E2 ELISA immunoassay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre coated with PGE2 protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin conjugated antibody specific to PGE2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is
The Prostaglandin E2 ELISA immunoassay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with PGE2 protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to PGE2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of PGE2 in the samples is then determined by comparing the OD of the samples to the standard curve.
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