Porcine TRACP-5b (Tartrate Resistant Acid Phosphatase 5b) ELISA Kit

Porcine TRACP-5b (Tartrate Resistant Acid Phosphatase 5b) ELISA KitThe Porcine TRACP 5b (Tartrate Resistant Acid Phosphatase 5b) ELISA immunoassay kit uses the Sandwich ELISA principle. The micro ELISA plate provided in this kit has been pre coated with an antibody specific to Porcine TRACP 5b. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Porcine TRACP 5b and Avidin Horseradish Peroxidase (HRP) conjugate are

The Porcine TRACP-5b (Tartrate Resistant Acid Phosphatase 5b) ELISA immunoassay kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Porcine TRACP-5b. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Porcine TRACP-5b and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Porcine TRACP-5b, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Porcine TRACP-5b. You can calculate the concentration of Porcine TRACP-5b in the samples by comparing the OD of the samples to the standard curve.