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Description
Mouse Ang-II (Angiotensin II) ELISA KitThe Mouse Ang II (Angiotensin II) ELISA immunoassay kit uses the Competitive ELISA principle. The micro ELISA plate provided in this kit has been pre coated with Mouse Ang II. Standards or samples are added to the appropriate micro ELISA plate wells then with a biotin conjugated antibody specific to Mouse Ang II. Next,Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is
The Mouse Ang-II (Angiotensin II) ELISA immunoassay kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with Mouse Ang-II. Standards or samples are added to the appropriate micro ELISA plate wells then with a biotin-conjugated antibody specific to Mouse Ang-II. Next,Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse Ang-II in the samples is then determined by comparing the OD of the samples to the standard curve.
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Exchange/Return Notes
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