SKU: 71742915389

Mouse IL-18 (Interleukin 18) high sensitivity ELISA Kit

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Description

Mouse IL-18 (Interleukin 18) high sensitivity ELISA KitThe Mouse IL 18 (Interleukin 18) high sensitivity ELISA Kit measures IL 18 in Mouse Serum, Plasma, Cell Culture Supernatant, Cell or Tissue Lysate. This kit is based on Double antibody Sandwich ELISA detection method and takes 4h assay time. The microplate provided in this kit has been precoated with anti IL 18 antibody. Add standard and properly diluted sample into relevant well respectively. After incubation, wash unbound components. Add

The Mouse IL-18 (Interleukin 18) high sensitivity ELISA Kit measures IL-18 in Mouse Serum, Plasma, Cell Culture Supernatant, Cell or Tissue Lysate. This kit is based on Double antibody-Sandwich ELISA detection method and takes 4h assay time. The microplate provided in this kit has been precoated with anti IL-18 antibody. Add standard and properly diluted sample into relevant well respectively. After incubation, wash unbound components. Add biotinylated detection antibody. Then, it binds with IL-18 bound to precoated antibody. Wash unbound components and add HRP-Streptavidin Conjugate (SABC). Wash unbound components again and add TMB substrate solution. Then, TMB was catalyzed by HRP to produce a blue color product that turned yellow after adding a stop solution. Read the O.D. absorbance at 450nm in a microplate reader. Calculate the concentration of IL-18 in the sample by plotting standard curve. The concentration of the target substance is proportional to the OD450 value.

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SKU: 71742915389

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4.5 ★★★★★
Based on 5 reviews
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J
Verified Purchase
Jaromir S.
Grantham, US
★★★★★ 5
Fits Sage Bambino Plus
Works flawlessly with Sage (Breville) Bambino Plus. It even comes with a straight basket which is an upgrade for the machine! People complaining about the magnets need to use their brains to turn first, pull second.
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on January 22, 2026
C
Verified Purchase
CRISTIAN T.
Dallas, US
★★★★★ 4
Magnets fell out. Everything else works well
Magnets holding the back piece fell out. Other than that it works as advertised.
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Reviewed in the United States on May 29, 2025
A
Verified Purchase
Albawab
Bozeman, US
★★★★★ 5
Really Cool
This is actually a well made portafilter. It was such a good idea to be able to have a nice WDT tool built in that doesn’t take up some extra counter space or anything. It minimizes clutter and just was a good idea so you always have it handy. Definitely would recommend.
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Reviewed in the United States on December 18, 2024
T
Verified Purchase
Ted Bush
Fort Morgan, US
★★★★★ 3
Fine basket, lousy magnets: Look elsewhere
Build is nice, solid and the baskets fit snuggly without being too hard to remove. The WDT tool works well and came with a surfeit of needles. HOWEVER The tiny magnets holding the WDT in the handle aren’t glued in place and now come FLYING out when the tool is removed. One or two pull free and then shoot across the unit, pulling others out and ending up somewhere in the kitchen. Fortunately (because they’re REALLY small), they form a small column making it somewhat easier to track down. But it’s such a nuisance I now leave the tool out, so there’s a small dangerous hedgehog of verrrry thin needles in my kitchen we need to work around. Sub-optimal, sorta defeats the purpose of in-handle tool.
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Reviewed in the United States on May 18, 2025
S
Verified Purchase
Sheri Luciano
Port Orchard, US
★★★★★ 5
Excellent product!
It’s all good
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Reviewed in the United States on February 27, 2025

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