SKU: 74689866740

Human PYGB (Glycogen phosphorylase, brain form) ELISA Kit

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Description

Human PYGB (Glycogen phosphorylase, brain form) ELISA KitThe Human PYGB (Glycogen phosphorylase, brain form) ELISA Kit measures PYGB in Human Serum, Plasma, Cell Culture Supernatant, Cell or Tissue Lysate. This kit is based on Double antibody Sandwich ELISA detection method and takes 3h assay time. The microplate provided in this kit has been precoated with anti PYGB antibody. Add standard and properly diluted sample into relevant well respectively. After incubation, wash unbound components. Add HRP

The Human PYGB (Glycogen phosphorylase, brain form) ELISA Kit measures PYGB in Human Serum, Plasma, Cell Culture Supernatant, Cell or Tissue Lysate. This kit is based on Double antibody-Sandwich ELISA detection method and takes 3h assay time. The microplate provided in this kit has been precoated with anti PYGB antibody. Add standard and properly diluted sample into relevant well respectively. After incubation, wash unbound components. Add HRP-detection antibody, then it binds with PYGB bound to precoated antibody. Wash unbound components and add TMB substrate solution. Then, TMB was catalyzed by HRP to produce a blue color product that turned yellow after adding a stop solution. Read the O.D. absorbance at 450nm in a microplate reader. Calculate the concentration of PYGB in the sample by plotting standard curve. The concentration of the target substance is proportional to the OD450 value.

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SKU: 74689866740

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4.5 ★★★★★
Based on 447 reviews
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Verified Purchase
Amazon Customer
Pawtucket, US
★★★★★ 5
Great book!
Format: Hardcover
My kids love this book. It's not a super long book but long enough to give a fun storyline. Puts a fun spin on cupid.
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Reviewed in the United States on February 24, 2026
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Verified Purchase
Leigha
Grantham, US
★★★★★ 5
Need this for your library
Format: Hardcover
Funny book. My class loves it.
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Reviewed in the United States on January 29, 2026
M
Michael Mateja
Birmingham, US
★★★★★ 5
Kids love the newest “Worst” tale-
Format: Hardcover
Kids will love this next book in The Worst series. Awesome, fun, colorful illustrations in this tale with Gilbert the Goblin trying to escape cupids and valentines. Instead of candy for Valentine’s Day give the gift of this book. My students love when Alex Willan has a new book out and are loving this one.
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Reviewed in the United States on January 17, 2026
H
Heidi Grange
Fort Morgan, US
★★★★★ 4
Plenty of fun for Gilbert fans!
Format: Hardcover
As in previous books in the series, Gilbert the Goblin complains about the problems he sees with Valentine's Day, what he calls 'mushy malarkey'. Young readers who don't care for the holiday will find much to relate to in the book. Although, Gilbert's complaints seem a tad trifling. Coconut chocolates are 'Blech'. He seems to have a special issue with the cupid's 'perfect', 'beautiful', and 'luxurious' hair. When Gilbert faces off with a group of cupids, he just barely manages to escape their love arrows, or so he thinks until he backs into a cactus in his own yard and believes he has been pricked by one such love arrow. He then becomes a goblin full of love. He loves his spells, his potions, even himself, but not coconut chocolates. He even welcomes his friends over to celebrate before bidding his pet sock slugs a fond goodnight. Good thing the love spell wears off. This funny book follows the pattern of the previous five books, but the addition of adorable cupids as well as Gilbert's beloved cactuses and pet sock slugs provides plenty of freshness as Gilbert once again (reluctantly) joins in the festivities. Plenty of fun for young fans of the series.
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Reviewed in the United States on February 10, 2026
P
Parent/Teacher
Pawtucket, US
★★★★★ 5
LOVE this book! ❤️
Format: Hardcover
I am a kindergarten teacher and my students LOVE this series! Valentines are the Worst is a new favorite! The illustrations are amazing as always and the story is funny and sweet, perfect for Valentine's Day!
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Reviewed in the United States on January 17, 2026

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