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Ships within 48 hours · Estimated delivery Aug 10 - Aug 15
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Description
POLY (ADP-Ribose) ELISA KitThe POLY (ADP Ribose) ELISA Kit measures Poly in Universal Cell Lysate. This kit is based on Competitive ELISA detection method and takes 2h assay time. The microplate provided in this kit has been precoated with Poly (ADP Ribose). Poly (ADP Ribose) in the sample or standard competes with a fixed amount of biotin labeled Poly (ADP Ribose) for sites on a pre coated antibody specific to Poly (ADP Ribose). Free components are washed off. After adding HRP
The POLY (ADP-Ribose) ELISA Kit measures Poly in Universal Cell Lysate. This kit is based on Competitive-ELISA detection method and takes 2h assay time. The microplate provided in this kit has been precoated with Poly (ADP-Ribose). Poly (ADP-Ribose) in the sample or standard competes with a fixed amount of biotin-labeled Poly (ADP-Ribose) for sites on a pre-coated antibody specific to Poly (ADP-Ribose). Free components are washed off. After adding HRP-Streptavidin Conjugate (SABC), biotin specifically binds with streptavidin to form immune complexes. Wash off unbound conjugate. Add TMB substrate solution. Then, TMB was catalyzed by HRP to produce a blue color product that turned yellow after adding a stop solution. Read the O.D. absorbance at 450nm in a microplate reader. Compare OD450 value of sample with the standard curve through curve fitting software to determine the concentration of Poly (ADP-Ribose) in the sample. The concentration of the target substance is inversely proportional to the OD450 value.
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