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Description
Rat NPHN (Nephrin) ELISA KitThe Rat (NPHN) Nephrin ELISA Kit measures Nephrin in Rat samples. The plate has been pre coated with Rat NPHS1 antibody. NPHS1 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Rat NPHS1 Antibody is added and binds to NPHS1 in the sample. Then Streptavidin HRP is added and binds to the Biotinylated NPHS1 antibody. After incubation unbound Streptavidin HRP is washed away during a washing step. Substrate
The Rat (NPHN) Nephrin ELISA Kit measures Nephrin in Rat samples. The plate has been pre-coated with Rat NPHS1 antibody. NPHS1 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Rat NPHS1 Antibody is added and binds to NPHS1 in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated NPHS1 antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Rat NPHS1. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.
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