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Description
Rat LUM (Lumican) ELISA KitThe Rat (LUM) Lumican ELISA Kit measures Lumican in Rat samples. The plate has been pre coated with Rat LUM antibody. LUM present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Rat LUM Antibody is added and binds to LUM in the sample. Then Streptavidin HRP is added and binds to the Biotinylated LUM antibody. After incubation unbound Streptavidin HRP is washed away during a washing step. Substrate solution is
The Rat (LUM) Lumican ELISA Kit measures Lumican in Rat samples. The plate has been pre-coated with Rat LUM antibody. LUM present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Rat LUM Antibody is added and binds to LUM in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated LUM antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Rat LUM. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.
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Exchange/Return Notes
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