SKU: 89230493949

Mouse IL1e (Interleukin 1 Epsilon) ELISA Kit (Small Sample)

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Ships within 48 hours · Estimated delivery Aug 13 - Aug 18

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Description

Mouse IL1e (Interleukin 1 Epsilon) ELISA Kit (Small Sample)The Mouse IL1e (Interleukin 1 Epsilon) ELISA Kit is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre coated with an antibody specific to Mouse IL1e. Standards or samples are added to the appropriate microtiter plate wells then with a biotin conjugated antibody specific to Mouse IL1e. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution

The Mouse IL1e (Interleukin 1 Epsilon) ELISA Kit is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse IL1e. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse IL1e. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse IL1e, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse IL1e in the samples is then determined by comparing the OD of the samples to the standard curve.

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SKU: 89230493949

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