SKU: 96594016964

Human SPHK1 (Sphingosine Kinase 1) ELISA Kit

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Description

Human SPHK1 (Sphingosine Kinase 1) ELISA KitThe Human (SPHK1) Sphingosine Kinase 1 ELISA Kit measures Sphingosine Kinase 1 in samples. The plate has been pre coated with Human SPHK1 antibody. SPHK1 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Human SPHK1 Antibody is added and binds to SPHK1 in the sample. Then Streptavidin HRP is added and binds to the Biotinylated SPHK1 antibody. After incubation unbound Streptavidin HRP is washed away

The Human (SPHK1) Sphingosine Kinase 1 ELISA Kit measures Sphingosine Kinase 1 in samples. The plate has been pre-coated with Human SPHK1 antibody. SPHK1 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Human SPHK1 Antibody is added and binds to SPHK1 in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated SPHK1 antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Human SPHK1. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.

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SKU: 96594016964

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