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Description
Rat IL2Ra ELISA KitProduct Specification Usage Experimental equipment required for the experiment: 1. Microplate reader (450nm) 2. High precision pipette and gun tips: 0. 5 10uL, 5 50uL, 20 200uL, 200 1000uL 3. 37 constant temperature box 4. Distilled water or deionized water Sample processing and requirements: 1. Serum: Place the whole blood sample collected in the serum separation tube at room temperature for 2 hours or at 4 overnight, then centrifuge at 1000g for 20
Product Specification
| Usage |
Experimental equipment required for the experiment: 1. Microplate reader (450nm) 2. High-precision pipette and gun tips: 0.5-10uL, 5-50uL, 20-200uL, 200-1000uL 3. 37℃ constant temperature box 4. Distilled water or deionized water Sample processing and requirements: 1. Serum: Place the whole blood sample collected in the serum separation tube at room temperature for 2 hours or at 4℃ overnight, then centrifuge at 1000×g for 20 minutes, and take the supernatant, or store the supernatant at -20℃ or -80℃, but avoid repeated freezing and thawing. 2. Plasma: Collect the specimen using EDTA or heparin as an anticoagulant. Centrifuge the specimen at 1000 × g for 15 minutes at 2-8°C within 30 minutes of collection. The supernatant can be assayed or stored at -20°C or -80°C, but avoid repeated freezing and thawing. 3. Tissue homogenization: Rinse the tissue with pre-chilled PBS (0.01M, pH 7.4) to remove residual blood (lysed red blood cells in the homogenate will affect the measurement results). Weigh the tissue and mince it. Add the minced tissue to the appropriate volume of PBS (generally a 1:9 weight-to-volume ratio, e.g., 1 g of tissue sample to 9 mL of PBS. The specific volume can be adjusted according to experimental needs and recorded. It is recommended to add protease inhibitors to the PBS) in a glass homogenizer and grind thoroughly on ice. To further lyse tissue cells, the homogenate can be sonicated or repeatedly frozen and thawed. Finally, centrifuge the homogenate at 5000 × g for 5-10 minutes, and the supernatant can be assayed. 4. Cell Lysis Buffer: Gently wash adherent cells with ice-cold PBS, then trypsinize and collect cells by centrifugation at 1000 × g for 5 minutes. Suspension cells can be collected directly by centrifugation. Wash the collected cells three times with ice-cold PBS and resuspend them in 150-200 μL of PBS per 1 × 10^6 cells (it is recommended to add protease inhibitors to the PBS; if the cell count is very low, reduce the PBS volume appropriately). Disrupt the cells by repeated freeze-thaw cycles or sonication. Centrifuge the extract at 1500 × g for 10 minutes at 2-8°C, and collect the supernatant for analysis. 5. Other biological fluids: Centrifuge at 1000xg for 20 minutes, remove the supernatant, and test. Pre-test preparation: 1. Remove the test kit from the refrigerator 10 minutes in advance and equilibrate to room temperature. 2. Prepare the standard gradient working solution: Add 1 mL of universal diluent to the lyophilized standard, let it stand for 15 minutes to completely dissolve, then gently mix (concentration is 10 ng/mL). Then dilute to the following concentrations: 10 ng/mL, 5 ng/mL, 2.5 ng/mL, 1.25 ng/mL, 0.625 ng/mL, 0.3125 ng/mL, 0.15625 ng/mL, and 0 ng/mL. Serial dilution method: Take seven EP tubes and add 500uL of universal diluent to each. Pipette 500uL of the 10ng/mL standard working solution into the first EP tube and mix thoroughly to make a 5ng/mL standard working solution. Repeat this procedure for subsequent tubes. The last tube serves as a blank well; there is no need to pipette liquid from the penultimate tube. See the figure below for details. 3. Preparation of biotinylated detection antibody working solution: Centrifuge the concentrated biotinylated antibody at 1000×g for 1 minute 15 minutes before use. Dilute the 100× concentrated biotinylated antibody to a 1× working concentration with universal diluent (e.g., 10uL concentrate + 990uL universal diluent). Prepare and use immediately. 4. Prepare the enzyme conjugate working solution: 15 minutes before use, centrifuge the 100× concentrated enzyme conjugate at 1000×g for 1 minute. Dilute the 100× concentrated HRP enzyme conjugate to a 1× working concentration with universal diluent (e.g., 10 μL of concentrate + 990 μL of universal diluent). Prepare immediately. 5. Prepare the 1× wash solution: Dispense 10 mL of 20× wash solution into 190 mL of distilled water (concentrated wash solution removed from the refrigerator may crystallize; this is normal. Allow to stand at room temperature until the crystals have completely dissolved before preparing). Procedure: 1. Remove the desired strips from the aluminum foil bag after equilibration at room temperature for 10 minutes. Seal the remaining strips in a ziplock bag and return to 4°C. 2. Sample addition: Add 100 μL of sample or standard of varying concentrations to the corresponding wells. Add 100 μL of universal diluent to the blank wells. Cover with a film and incubate at 37°C for 60 minutes. (Recommendation: Dilute the sample to be tested at least 1-fold with universal diluent before adding it to the ELISA plate. This will reduce the impact of matrix effects on the test results. The sample concentration should be multiplied by the corresponding dilution factor when calculating the final sample concentration. It is recommended to run replicates for all test samples and standards.) 3. Add Biotinylated Antibody: Remove the ELISA plate and discard the liquid without washing. Add 100 μL of Biotinylated Antibody Working Solution directly to each well. Cover with a film and incubate at 37°C for 60 minutes. 4. Wash: Discard the liquid and add 300 μL of 1x Wash Solution to each well. Let stand for 1 minute, shake off the wash solution, and pat dry on absorbent paper. Repeat this process three times (a plate washer can also be used). 5. Add Enzyme Conjugate Working Solution: Add 100 μL of Enzyme Conjugate Working Solution to each well. Cover with a film and incubate at 37°C for 30 minutes. 6. Washing: Discard the liquid and wash the plate five times as in step 4. 7. Adding substrate: Add 90 μL of substrate (TMB) to each well, cover with a sealing film, and incubate at 37°C in the dark for 15 minutes. 8. Adding stop solution: Remove the ELISA plate and add 50 μL of stop solution directly to each well. Immediately measure the OD value of each well at a wavelength of 450 nm. Calculating experimental results: 1. Calculate the average OD value of the standard and sample replicates and subtract the OD value of the blank well as a correction factor. Plot the standard curve of the four-parameter logistic function on double-logarithmic graph paper, with concentration as the horizontal axis and OD value as the vertical axis. 2. If the sample OD value is higher than the upper limit of the standard curve, dilute the sample appropriately and retest. Multiply the sample concentration by the corresponding dilution factor. |
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| Theory | This kit utilizes a double-antibody sandwich enzyme-linked immunosorbent assay (ELISA). Sample, standard, biotinylated detection antibody, and HRP conjugate are sequentially added to microwells pre-coated with Interleukin 2 Receptor Alpha (IL2Ra) capture antibody. After incubation and washing, the sample is developed using the substrate TMB. TMB is converted to blue by peroxidase (HRP) catalysis and to yellow by acid. The intensity of the color is positively correlated with the amount of Interleukin 2 Receptor Alpha (IL2Ra) in the sample. The absorbance (OD) is measured at 450 nm using a microplate reader to calculate the sample concentration. | |||||||||||||||||||||||||||||||||
| Source | Rat | |||||||||||||||||||||||||||||||||
| Synonym | Rat Interleukin 2 Receptor Alpha ELISA Kit | |||||||||||||||||||||||||||||||||
| Detection Type | Double antibody sandwich method | |||||||||||||||||||||||||||||||||
| Composition |
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| Background | The interleukin-2 receptor alpha chain (also known as CD25) is a protein encoded by the IL2RA gene. The interleukin-2 (IL2) receptor alpha chain (IL2RA) and beta chain (IL2RB), together with the common gamma chain (IL2RG), constitute the high-affinity IL2 receptor. Homodimeric alpha chains (IL2RA) result in a low-affinity receptor, while homodimeric beta chains (IL2RB) produce a medium-affinity receptor. Soluble IL2RA is an integral membrane protein that has been isolated and identified as a result of extracellular proteolysis. It is a type I transmembrane protein found in activated T cells, activated B cells, some thymocytes, myeloid precursors, and oligodendrocytes. IL2RA is expressed in most B-cell tumors, some acute nonlymphocytic leukemias, neuroblastoma, mastocytosis, and tumor-infiltrating lymphocytes. | |||||||||||||||||||||||||||||||||
| General Notes | 1. Strictly adhere to the specified incubation time and temperature to ensure accurate results. All reagents must be at room temperature (20-25°C) before use. Refrigerate reagents immediately after use. 2. Improper plate washing may result in inaccurate results. Ensure that all liquid in the wells is aspirated thoroughly before adding substrate. Do not allow the wells to dry out during incubation. 3. Remove any residual liquid and fingerprints from the bottom of the plate, as this will affect the OD value. 4. The substrate developer solution should be colorless or very light in color. Do not use substrate solution that has turned blue. 5. Avoid cross-contamination of reagents and specimens to prevent erroneous results. 6. Avoid direct exposure to strong light during storage and incubation. 7. Do not expose any reagents to bleaching solvents or the strong fumes emitted by bleaching solvents. Any bleaching agent will destroy the biological activity of the reagents in the kit. 8. Do not use expired products, and do not mix components with different product numbers and batches. 9. Recombinant proteins from sources other than the kit may not be compatible with the antibodies in this kit and will not be recognized. 10. If there is a possibility of disease transmission, all samples should be managed properly and samples and testing devices should be handled according to prescribed procedures. |
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| Storage Temp. | If the unopened kit is stored at 4°C, the shelf life is 6 months. | |||||||||||||||||||||||||||||||||
| Test Range | 0.15-10 ng/mL | |||||||||||||||||||||||||||||||||
| Applications | Serum, plasma, tissue homogenates, cell lysates and other biological fluids |
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4.6 ★★★★★
Based on 10 reviews
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Product Reviews
★★★★★ 5
"Racial Capitalism"
Format: Paperback
Cedric J. Robinson’s Black Marxism is first a history of Black people appearing in historical texts as far back as Herodotus (c. 484 – c. 425 BCE) in ancient Greece, and second a history of “the collisions of the Black and white ‘races’ beginning in the fifteenth and sixteenth centuries.” Robinson’s thesis connects the evolution of capitalism to its roots in racism (racialism) understood in broad terms to comprise the subjugation of one class/group/nation/race by another (the Irish by the English in the nineteenth century, for example). He uses the term “racial capitalism” to express this process—the necessity of opposing classes for the function of capitalism. As a result, “racialism,” he says, “would inevitably permeate the social structures emergent from capitalism.” Keynes attributed the slow change in the “standard of life of the average man” until the beginning of the eighteenth century to “the remarkable absence of important technical improvements and to the failure of capital to accumulate.” Capital is accumulated, in Marx’s view, through the accretion of “surplus labor” which is the extra time a worker “must add to the working time necessary for his own maintenance . . . in order to produce the means of subsistence for the owners of the means of production.” Robinson ties capitalism’s early exploitation of surplus labor to slave labor and the slave trade noting, “historically, slavery was a critical foundation for capitalism.” Robinson traces the forced transport of Black people from Africa (the diaspora) to Europe, as well as Central, South, and North America as a foundation of early capitalism (and slavery as its form of “primitive accumulation” of capital). In his discussions of slavery, Robinson stresses the sense of the enslaved people with respect to their captors in terms of the slaves’ resistance, hostility, and defiance of the masters—their “Black radicalism.” As Robinson’s text approaches the twentieth century and the influence of Marx, his focus narrows to the significance and character of specific Black leaders including W. E. B. Du Bois, C. L. R. James, and Richard Wright and their respective connections to Marxism’s diverse interpretations. Marxism, says Robinson, “has proven insufficiently radical to expose and root out the racialist order that contaminates its analytic and philosophic applications or to come to effective terms with the implications of its own class origins.”
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Reviewed in the United States on September 2, 2022
★★★★★ 5
Any socialist movement must centrally address racial liberation to succeed.
Format: Kindle
Robinson's masterwork powerfully demonstrates how the Black radical tradition emerged from the shared experiences of resistance to racial capitalism and colonialism. By tracing this intellectual and political lineage through figures like W.E.B. Du Bois, C.L.R. James, and Richard Wright, Robinson shows that Black liberation struggles were not simply an offshoot of European socialism, but represented their own distinctive radical tradition.
A key insight is how Black resistance movements developed theoretical frameworks and modes of struggle that went beyond traditional Marxist analysis. Where European Marxism focused primarily on class conflict within industrial capitalism, Black radical thinkers recognized that racial oppression was fundamental to how capitalism developed globally through colonialism and slavery. This more comprehensive analysis helped explain why racial liberation had to be central to any meaningful socialist transformation in the United States.
The book compellingly argues that Black liberation movements - from slave rebellions to civil rights to Black Power - represented some of the most significant challenges to American capitalism. These struggles exposed how racial oppression was not incidental but essential to American economic and social relations. By fighting for racial justice, these movements struck at the foundations of the capitalist order itself.
Robinson's updated edition strengthens these arguments by extending the analysis into more recent decades. He examines how Black radical politics evolved in response to neoliberalism and continued racial inequalities, while maintaining connections to earlier traditions of resistance.
For readers interested in both racial justice and socialist politics, this book remains invaluable for understanding how these struggles are fundamentally interconnected. It demonstrates why any socialist movement in the United States must centrally address racial liberation to succeed in transforming society.
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Reviewed in the United States on November 11, 2024
★★★★★ 5
A Classic That Requires Time
Format: Paperback
This book is for a particular type of reader. Robinson’s writing is beautiful, but not easy. The ideas are complex. It takes effort to get through. But, if you are interested in Black politics, and looking for fresh thinking, I recommend it highly.
The funny thing is, the title is misleading. It is more about Europe and the formation of capitalism, and what Robinson defines as The Black Radical Tradition. Marx is critiqued but not rejected, and held uneasily at arm’s length.
As Angela Davis wrote, this book needs to be read more than once. It’s like an album or a movie that is so unique and rich that you know you probably missed something on the first go-round. I expect to return to it many years to come.
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Reviewed in the United States on November 15, 2023
★★★★★ 5
Great condition
Format: Paperback
It came one day too late for Christmas, but that wasn't promised. Otherwise, it was received in great condition.
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Reviewed in the United States on January 1, 2022
★★★★★ 5
Exceptional
Format: Paperback
Glad I purchased this book for my collection. Great information. Knowledge is power.
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Reviewed in the United States on January 3, 2026