Human IFN-α High Sensitivity ELISA Kit

Detection Principle:

This kit uses double antibody sandwich ELISA technology. Specific anti human ifn-α Capture antibody was pre coated on a high affinity microplate. Add the standard, the sample to be tested and the biotin labeled detection antibody into the wells of the enzyme plate in turn, shake well and mix well, and then place it at room temperature for 2 hours of incubation process. The ifn-&alpha in the sample; Bound to solid-phase antibody and detection antibody. After washing sufficiently to remove free and unbound components, streptavidin HRP (sa-hrp) labeled with horseradish peroxidase was added. After washing, signal enhancer was added for incubation. After washing to remove unbound material, sa-hrp was added again. After washing again, TMB chromogenic substrate was added and incubated at room temperature in the dark to develop color. The depth of color response and ifn-&alpha in the sample; There is a positive correlation between the concentration of. Add stop solution to stop the reaction, and use a microplate reader to measure the absorbance value at 450 nm detection wavelength (correction wavelength 570-630 nm).

Detection Type: Double antibody sandwich method

Form: pre coated 96 well plate

Test Sample Type: cell supernatant, serum, plasma

Loading Amount: 100 μ L

Kit Components: Pre coated 96 well plates, standards, ifn-α One copy of detection antibody, standard dilution, detection buffer, signal enhancer, signal enhancer dilution, TMB chromogenic substrate, washing solution, termination solution, sa-hrp, plate sealing membrane and instructions.

Sensitivity: 0.23 pg/ml

Detection Range: 7.81-500 pg/ml

Recovery Range: 95-119%

Storage Method: 2-8 ℃

Standard Curve:

Background:

Interferons (IFNs) are host cells that respond to pathogens (such as viruses, bacteria, parasites or tumor cells)A group of signaling proteins that are synthesized and released when. Under normal circumstances, virus-infected cells will release interferon, making the surrounding cells improve their antiviral defense ability. Based on the receptor type, human interferons can be divided into three types: type I interferons, including ifn-&alphaIfn-&betaIfn-&epsilonIfn-κ And ifn-ω, Type II interferon (known as ifn-γ in humans)And type III interferons. Ifn-α The protein is produced by leukocytes and is mainly involved in innate immunity in response to viral infection. Studies have shown that enhancing ifn-&alpha in tumor infiltrating macrophages; The expression of Bcl-2 can cause more effective dendritic cell activation and immune effector cytotoxicity. Ifn-α As a thermogenic factor, it can cause fever by changing the activity of heat sensitive neurons in the hypothalamus. Using a similar mechanism, ifn-α Can be used to relieve pain, and &mu-Opioid receptors interact to act as analgesics.