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Description
Human NOTCH1 ELISA KitProduct Specification Usage Experimental equipment required for the experiment: 1. Microplate reader (450nm) 2. High precision pipette and gun tips: 0. 5 10uL, 5 50uL, 20 200uL, 200 1000uL 3. 37 constant temperature box 4. Distilled water or deionized water Sample processing and requirements: 1. Serum: Place the whole blood sample collected in the serum separation tube at room temperature for 2 hours or at 4 overnight, then centrifuge at 1000g for 20
Product Specification
| Usage |
Experimental equipment required for the experiment: 1. Microplate reader (450nm) 2. High-precision pipette and gun tips: 0.5-10uL, 5-50uL, 20-200uL, 200-1000uL 3. 37℃ constant temperature box 4. Distilled water or deionized water Sample processing and requirements: 1. Serum: Place the whole blood sample collected in the serum separation tube at room temperature for 2 hours or at 4℃ overnight, then centrifuge at 1000×g for 20 minutes, and take the supernatant, or store the supernatant at -20℃ or -80℃, but avoid repeated freezing and thawing. 2. Plasma: Collect the specimen using EDTA or heparin as an anticoagulant. Centrifuge the specimen at 1000 × g for 15 minutes at 2-8°C within 30 minutes of collection. The supernatant can be assayed or stored at -20°C or -80°C, but avoid repeated freezing and thawing. 3. Tissue homogenization: Rinse the tissue with pre-chilled PBS (0.01M, pH 7.4) to remove residual blood (lysed red blood cells in the homogenate will affect the measurement results). Weigh the tissue and mince it. Add the minced tissue to the appropriate volume of PBS (generally a 1:9 weight-to-volume ratio, e.g., 1 g of tissue sample to 9 mL of PBS. The specific volume can be adjusted according to experimental needs and recorded. It is recommended to add protease inhibitors to the PBS) in a glass homogenizer and grind thoroughly on ice. To further lyse tissue cells, the homogenate can be sonicated or repeatedly frozen and thawed. Finally, centrifuge the homogenate at 5000 × g for 5-10 minutes, and the supernatant can be assayed. 4. Cell Lysis Buffer: Gently wash adherent cells with ice-cold PBS, then trypsinize and collect cells by centrifugation at 1000×g for 5 minutes. Suspension cells can be collected directly by centrifugation. Wash collected cells three times with ice-cold PBS and resuspend in 150-200 μL of PBS per 1×10^6 cells (it is recommended to add protease inhibitors to the PBS; if the cell count is very low, reduce the PBS volume appropriately). Disrupt the cells by repeated freeze-thaw cycles or sonication. Centrifuge the extract at 1500×g for 10 minutes at 2-8°C, and remove the supernatant for analysis. 5. Cell Culture Supernatant: Centrifuge at 1000×g for 20 minutes. Remove the supernatant for analysis or store at -20°C or -80°C, avoiding repeated freeze-thaw cycles. 6. Other biological fluids: Centrifuge at 1000xg for 20 minutes, remove the supernatant, and test. Pre-test preparation: 1. Remove the test kit from the refrigerator 10 minutes in advance and equilibrate to room temperature. 2. Prepare the standard gradient working solution: Add 1 mL of universal diluent to the lyophilized standard, let it stand for 15 minutes to completely dissolve, then gently mix (concentration is 10 ng/mL). Then dilute to the following concentrations: 10 ng/mL, 5 ng/mL, 2.5 ng/mL, 1.25 ng/mL, 0.625 ng/mL, 0.3125 ng/mL, 0.15625 ng/mL, and 0 ng/mL. Serial dilution method: Take seven EP tubes and add 500uL of universal diluent to each. Pipette 500uL of the 10ng/mL standard working solution into the first EP tube and mix thoroughly to make a 5ng/mL standard working solution. Repeat this procedure for subsequent tubes. The last tube serves as a blank well; there is no need to pipette liquid from the penultimate tube. See the figure below for details. 3. Preparation of biotinylated detection antibody working solution: Centrifuge the concentrated biotinylated antibody at 1000×g for 1 minute 15 minutes before use. Dilute the 100× concentrated biotinylated antibody to a 1× working concentration with universal diluent (e.g., 10uL concentrate + 990uL universal diluent). Prepare and use immediately. 4. Prepare the enzyme conjugate working solution: 15 minutes before use, centrifuge the 100× concentrated enzyme conjugate at 1000×g for 1 minute. Dilute the 100× concentrated HRP enzyme conjugate to a 1× working concentration with universal diluent (e.g., 10 μL of concentrate + 990 μL of universal diluent). Prepare immediately. 5. Prepare the 1× wash solution: Dispense 10 mL of 20× wash solution into 190 mL of distilled water (concentrated wash solution removed from the refrigerator may crystallize; this is normal. Allow to stand at room temperature until the crystals have completely dissolved before preparing). Procedure: 1. Remove the desired strips from the aluminum foil bag after equilibration at room temperature for 10 minutes. Seal the remaining strips in a ziplock bag and return to 4°C. 2. Sample addition: Add 100 μL of sample or standard of varying concentrations to the corresponding wells. Add 100 μL of universal diluent to the blank wells. Cover with a film and incubate at 37°C for 60 minutes. (Recommendation: Dilute the sample to be tested at least 1-fold with universal diluent before adding it to the ELISA plate. This will reduce the impact of matrix effects on the test results. The sample concentration should be multiplied by the corresponding dilution factor when calculating the final sample concentration. It is recommended to run replicates for all test samples and standards.) 3. Add Biotinylated Antibody: Remove the ELISA plate and discard the liquid without washing. Add 100 μL of Biotinylated Antibody Working Solution directly to each well. Cover with a film and incubate at 37°C for 60 minutes. 4. Wash: Discard the liquid and add 300 μL of 1x Wash Solution to each well. Let stand for 1 minute, shake off the wash solution, and pat dry on absorbent paper. Repeat this process three times (a plate washer can also be used). 5. Add Enzyme Conjugate Working Solution: Add 100 μL of Enzyme Conjugate Working Solution to each well. Cover with a film and incubate at 37°C for 30 minutes. 6. Washing: Discard the liquid and wash the plate five times as in step 4. 7. Adding substrate: Add 90 μL of substrate (TMB) to each well, cover with a sealing film, and incubate at 37°C in the dark for 15 minutes. 8. Adding stop solution: Remove the ELISA plate and add 50 μL of stop solution directly to each well. Immediately measure the OD value of each well at a wavelength of 450 nm. Calculating experimental results: 1. Calculate the average OD value of the standard and sample replicates and subtract the OD value of the blank well as a correction factor. Plot the standard curve of the four-parameter logistic function on double-logarithmic graph paper, with concentration as the horizontal axis and OD value as the vertical axis. 2. If the sample OD value is higher than the upper limit of the standard curve, dilute the sample appropriately and retest. Multiply the sample concentration by the corresponding dilution factor. |
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| Theory | This kit utilizes a double-antibody sandwich enzyme-linked immunosorbent assay (ELISA). Sample, standard, biotin-labeled detection antibody, and HRP conjugate are sequentially added to microwells pre-coated with a capture antibody against Translocation Associated Notch Homolog 1 (NOTCH1). After incubation and washing, the sample is developed using the substrate TMB. TMB is converted to blue by peroxidase (HRP) catalysis and to yellow by acid. The intensity of the color is positively correlated with the amount of Translocation Associated Notch Homolog 1 (NOTCH1) in the sample. The absorbance (OD) is measured at 450 nm using a microplate reader to calculate the sample concentration. | |||||||||||||||||||||||||||||||||
| Source | Human | |||||||||||||||||||||||||||||||||
| Synonym | Human Translocation Associated Notch Homolog 1 ELISA Kit | |||||||||||||||||||||||||||||||||
| Detection Type | Double antibody sandwich method | |||||||||||||||||||||||||||||||||
| Composition |
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| Background | Notch homolog 1, associated with translocation, also known as NOTCH1, encodes a protein that is a single-pass transmembrane receptor. This gene is a member of the Notch family. Members of this type 1 transmembrane protein family share common structural features, including an extracellular domain composed of multiple epidermal growth factor (EGF)-like repeats and an intracellular domain composed of multiple distinct domain types. | |||||||||||||||||||||||||||||||||
| General Notes | 1. Strictly adhere to the specified incubation time and temperature to ensure accurate results. All reagents must be at room temperature (20-25°C) before use. Refrigerate reagents immediately after use. 2. Improper plate washing may result in inaccurate results. Ensure that all liquid in the wells is aspirated thoroughly before adding substrate. Do not allow the wells to dry out during incubation. 3. Remove any residual liquid and fingerprints from the bottom of the plate, as this will affect the OD value. 4. The substrate developer solution should be colorless or very light in color. Do not use substrate solution that has turned blue. 5. Avoid cross-contamination of reagents and specimens to prevent erroneous results. 6. Avoid direct exposure to strong light during storage and incubation. 7. Do not expose any reagents to bleaching solvents or the strong fumes emitted by bleaching solvents. Any bleaching agent will destroy the biological activity of the reagents in the kit. 8. Do not use expired products, and do not mix components with different product numbers and batches. 9. Recombinant proteins from sources other than the kit may not be compatible with the antibodies in this kit and will not be recognized. 10. If there is a possibility of disease transmission, all samples should be managed properly and samples and testing devices should be handled according to prescribed procedures. |
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| Storage Temp. | If the unopened kit is stored at 4°C, the shelf life is 6 months. | |||||||||||||||||||||||||||||||||
| Test Range | 0.156-10 ng/mL | |||||||||||||||||||||||||||||||||
| Applications | Serum, plasma, tissue homogenate, cell lysate, cell culture supernatant and other biological fluids |
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4.1 ★★★★★
Based on 9 reviews
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Product Reviews
★★★★★ 5
Great replacement micro needling cartridges.
Picked today up for replacements for my Dr Pen micro needler.
They arrived individually sealed, ready for use. They appear to be compatible good quality product just like my originals. Love the price point and savings.
They fit my device perfectly.
These arrived at the Dr Pen cartridge box although do not have Dr Pen brand on cartridges appear high-quality similar in packaging of the originals. They do not have the pen, cap safety guard, but that's really not a needed part of the product.
Make sure to verify you have the right device.
Be aware they do not fit correctly in M8 devices.
Great value for the money you get 21 piece micro, needling cartridges, various needle styles. The kit comes with 36 needle, 12 needle and nano cartridges.
I have already used the Nano cartridge and they are super comparable to original cartridges quality, They do not drag on the skin easily move and dispense face serum product.
So far, I've been super happy with the replacements.
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Reviewed in the United States on May 29, 2026
★★★★★ 5
21 Piece Pen Cartridges
This 21 piece microneedling cartridge set is an excellent value and gives a lot of versatility for different skincare needs. I really like that it includes three different tip styles: nano, 12 pin, and 36 pin. There are 7 of each variety included, which makes it easy to customize treatments depending on what area I’m focusing on and how gentle or intensive I want the session to be.
The cartridges fit my Dr Pen device well and feel securely attached during use. Everything came individually packaged and clean, which is important for at home microneedling routines. The nano tips are great for product infusion and glow-focused treatments, while the 12 and 36 pin options work well for more targeted skin texture care. Overall, this is a convenient and affordable replacement set that’s perfect for anyone wanting multiple cartridge options in one package.
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Reviewed in the United States on May 22, 2026
★★★★★ 4
Wish I Knew if it is Surgical Grade Steel
I have a Dr. Pen A6, and these non-OEM cartridges are a great value. You receive twenty-one cartridges in various sizes, and each cartridge is individually wrapped.
These pins come in three sizes: thirty-six pin, twelve pin, and nano. I had no issue inserting the cartridge and locking it into place, and I didn't notice any wiggling or looseness. The pins were sharp and did not tear or drag on my skin. Of course, you will see small pinpricks where you have treated.
One thing I would like to know is whether these are made with surgical grade steel. I would prefer that they are, but I don't see that information anywhere in the listing. That said, I didn't notice any unusual irritation.
I now have a good variety of cartridges to be more consistent with my pen usage. I will update this review if I notice anything related to the steel used.
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Reviewed in the United States on May 19, 2026
★★★★★ 5
Perfect for Husbands but Wives may Enjoy Reading it Too!
For a long time I've wondered what my love language is in particular because they all sound good to me. I love receiving stuffed animals from my husband so maybe it is gifts. But then I also adore undivided attention when we watch movies nearly every night, so maybe it is quality time. When my husband empties the dishwasher that is good so maybe it is acts of service. Well how about a compliment, that is nice too. I think I'm greedy, I want it all. lol So don't think your wife will only like just one love language. Maybe she wants touch, quality time and acts of service too.
This book explains what the typical differences are in what men and women want in regards to love languages. It also wisely explains how to apologize to a woman in a sincere way that will make her melt. It deals with anger and women want less of that!
At the end of the book there are two tests, one for him, one for her. You can then figure out what each other's love languages are. As the books says, sometimes you are bilingual. Also there are various dialects.
This book also makes you realize why sometimes the love language works and at other times it does not get the points you think you are getting. Like when a woman is really busy, some things mean more than others. While she's in the kitchen she might want help with the kids instead of touch or when she is feeling down she might want a compliment to make her feel appreciated.
So I think learn all the love languages and use them wisely. I'm a fan! Any man who adds these languages to his skill set is going to be way more desired than usual. Women pay attention and this is a way of getting your point across. Oh and at the end of the chapters there is an easy "phrase book" so you get tips on specific things you can do to speak each language.
I'm giving this book to my husband and I really hope he reads it! I'm also happy I read it because I've got myself more figured out. To my husband I say more love languages please!!!!
~The Rebecca Review
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Reviewed in the United States on August 18, 2024
★★★★★ 5
Good book
Good book
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Reviewed in the United States on February 23, 2026