SKU: 17535841232

Human IL-5 ELISA Kit

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Description

Human IL-5 ELISA KitProduct Specification Description Detection Principle: This kit uses double antibody sandwich ELISA technology. The specific anti human IL 5 capture antibody was pre coated on a high affinity microplate. Add the standard, the sample to be tested and the biotin labeled detection antibody into the wells of the enzyme plate in turn, shake well and mix well, and then place it at room temperature for 2 hours of incubation process. The IL 5 in the sample

Product Specification

Description

Detection Principle:

This kit uses double antibody sandwich ELISA technology. The specific anti-human IL-5 capture antibody was pre coated on a high affinity microplate. Add the standard, the sample to be tested and the biotin labeled detection antibody into the wells of the enzyme plate in turn, shake well and mix well, and then place it at room temperature for 2 hours of incubation process. The IL-5 in the sample combines with the solid-phase antibody and the detection antibody. After washing sufficiently to remove free and unbound components, streptavidin HRP (sa-hrp) labeled with horseradish peroxidase was added. After washing again, TMB chromogenic substrate was added and incubated at room temperature in the dark to develop color. The depth of color response is positively correlated with the concentration of IL-5 in the sample. Add stop solution to stop the reaction, and use a microplate reader to measure the absorbance value at 450 nm detection wavelength (correction wavelength 570-630 nm).

Detection Type: Double antibody sandwich method

Form: pre coated 96 well plate

Test Sample Type: cell supernatant, serum, plasma

Loading Amount: 100 μ L

Kit Components:A copy of pre coated 96 well plate, standard, IL-5 detection antibody, standard dilution, detection buffer, TMB chromogenic substrate, washing solution, termination solution, sa-hrp, plate sealing membrane and instructions.

Sensitivity: 0.76pg/ml

Detection Range: 15.63-1000 pg/ml

Recovery Range: 91-107%

Storage Method: 2-8 ℃

Standard Curve:

Background:

Interleukin 5 (IL-5) is a Th2 cytokine member of hematopoietic family, which is composed of 115 amino acids. It is similar to other members of this cytokine family (IL-3 and GM-CSF)Different, the activated form of this glycoprotein is homodimer. IL-5 is secreted by Th2 cells and mast cells. Its biological activity on B cells and other types of cells shows that IL-5 has an important functional effect on the activity of Th2 cells. IL-5 stimulates B cell growth and increases immunoglobulin secretion by binding to its receptor. It is also a key factor in activating eosinophils. IL-5 has been associated with a variety of allergic diseases, including allergic rhinitis and asthma. A large increase in IL-5 was observed in circulating tissues and tracheal tissues, while sputum eosinophils decreased.

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SKU: 17535841232

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4.2 ★★★★★
Based on 14 reviews
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A
Verified Purchase
Ashton Gibson
Pawtucket, US
★★★★★ 5
Take the skin off and get the right size for your dog’s weight!
Color: Panda Bear - Large, Color: Panda Bear - Large
This review is specifically for the panda toy. My dog is a super chewer and will literally go through any normal toy, even the ones labeled for aggressive chewers, within a few hours at most. He’s about to be 10 years old and this is the first time he’s ever had a toy that lasted as long as it has. I wanna mention the outer layer he almost immediately chewed through. To me, it wasn’t worth the fear of him dealing through pieces so I just took the whole “skin” off. However, he’s safely had this for 2 months with no issues and heavy daily chewing until he finally broke it apart. So if you have a super chewer, make sure to get the size appropriate for your dog’s weight and take the “skin” off and you’re left with a quality toy that will stick around! Pictured is a set of 2 new toys of the same variety since he finally chewed through his last batch lol
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on May 27, 2026
K
Verified Purchase
Kit
Port Orchard, US
★★★★★ 4
My dog loves this toy but…
Color: Grey Bear - Large
This toy has many pros and cons, but it’s my dogs all time favorite toy so I have to give it 4 stars. Pro:My German Shepard loves this toy, it has no squeaker and she hates squeakers, so she hasn’t tried to destroy it. It’s the perfect size for a large ball. She likes to throw her toys and this one has a pretty decent bounce to it so she can entertain herself. Cons: the inside is hard rubber, she likes to throw her toys and it hurts when she hits you with it or drops it on your feet. Also a huge hazard to windows and anything breakable.
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Reviewed in the United States on July 12, 2025
I
Verified Purchase
ingrid C
Los Angeles, US
★★★★★ 5
Pretty awesome chew toy
Color: Grey Bear - Large
This toy is great!! I have a doodle that shreds everything in sight, including durable toys, but this one stood up to the Teddy test! He easily ripped off the ear/feet bits, but the fabric casing hasn’t come off after a week of play, and I expect the rubber toy inside will be even harder to break. This is a fairly heavy toy, so it makes a thunk when it drops and is probably not suitable for small dogs. It has a light squeak that is quiet and infrequent enough to not annoy me, and it’s pretty cute too!! it does get soaked in drool pretty fast (and takes forever to dry), but at this point i’m grateful it lasts long enough to get drooled on at all.
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Reviewed in the United States on September 30, 2025
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Verified Purchase
a b
Chelsea, US
★★★★★ 3
less than 24 hours :/
Color: Grey Bear - Large, Color: Grey Bear - Large
smaller than i expected but had a strong interior that i was pleasantly surprised with. my lil lady very much enjoyed it, however it lasted less than 24 hours before the poor toy was skinned alive :( she loves plushies and she’s obsessed w his skin after tearing him apart. it does have a solid rubber interior that is still good as a toy, however i was hoping its skin would stay on longer than a day :( overall decent toy, 3 stars for being destroyed so quickly. wouldn’t recommend for strong chewers
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Reviewed in the United States on August 7, 2025
J
Verified Purchase
Jen
Los Angeles, US
★★★★★ 5
Lasts long
Color: Panda Bear - Large
Perfect for super chewer dogs.
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on May 14, 2026

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