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Description
Human Prostate Cancer Organoid Culture Medium KitProduct Specification Usage 1. Primary (1) The extracted tissue must be placed in a pre cooled (2 8 C) tissue preservation solution E sampling bottle, quickly transported to a clean laboratory for tissue treatment and cell separation, photographed, and registered with information (2) Prepare several culture dishes and add 4 pre cooled primary culture buffer B for later use (3) Disinfect the sampling bottle, place the tissue in a culture dish, clean it
Product Specification
| Usage |
1. Primary (1) The extracted tissue must be placed in a pre cooled (2-8° C) tissue preservation solution E sampling bottle, quickly transported to a clean laboratory for tissue treatment and cell separation, photographed, and registered with information (2) Prepare several culture dishes and add 4 ℃ pre cooled primary culture buffer B for later use (3) Disinfect the sampling bottle, place the tissue in a culture dish, clean it three times with primary culture buffer B, and then use an ophthalmic scissors or surgical knife to cut the tissue into tissue blocks with a volume of approximately 1-3mm (4) The tissue was digested with the digestion solution C of the primary tissue of the porcine endometrium, and shaken at 37 ℃ for 10-20 minutes (the digestion process was observed at any time) (5) Take a small amount of liquid and observe under a microscope. After observing a large number of individual cells or cell clusters below 70um, add three times the volume of primary culture buffer B to terminate digestion (6) Filter using a 100um pore sieve, collect the filtrate, concentrate and centrifuge at 300g for 5 minutes, remove the supernatant, add primary culture buffer B, and resuspend and centrifuge (7) Matrix adhesive calculation: After step 6, observe the collected tissue volume and add 25 times the tissue volume of matrix adhesive (ABS9495) to resuspend the board 2. Organ like passage culture (1) Pipette the culture medium and add 1-2ml of 4 ℃ organ like passage culture buffer G to each well for 2 minutes (2) Gently blow the matrix adhesive with a pipette, collect it in a 15ml centrifuge tube, and let it stand at 4 ℃ for 10 minutes. (Each 6-8 wells is a group) (3) a: If the number of organoids is insufficient or the volume is small, centrifuge for 5 minutes to discard the supernatant. Add an appropriate amount of organoid passage culture buffer G and resuspend it into a 1.5ml centrifuge tube. Centrifuge for 5 minutes to discard the liquid and proceed to step 4 b: When the number of organoids is large or the volume is large: centrifuge for 5 minutes to discard the supernatant, add an appropriate amount of organoid passage digestion solution D for 2-3 minutes, add organoid passage culture buffer G to terminate digestion, centrifuge for 5 minutes to discard the mixture, add an appropriate amount of organoid passage culture buffer G and resuspend into a 1.5ml centrifuge tube, centrifuge for 5 minutes to discard the liquid for step 4 3. Organ like cryopreservation (1) Pipette the culture medium and add 1-2ml of 4 ℃ organ like passage culture buffer G to each well for 2 minutes (2) Gently blow the matrix adhesive with a pipette, collect it in a 15ml centrifuge tube, and let it stand at 4 ℃ for 10 minutes. (Each 6-8 wells is a group) (3) Centrifuge for 5 minutes to discard the supernatant, add an appropriate amount of organoid passage culture buffer G, and resuspend again. Centrifuge for 5 minutes at 300g to discard the liquid (4) Add an appropriate amount of organoid cryopreservation solution F, gently blow and resuspend, taking a 24 well cell culture plate as an example: the density is 2 wells, and one tube is frozen, with a volume of 1.4ml per tube (5) Mark the information properly, cool down the program, and then transfer it to liquid nitrogen for long-term storage 4. Organ like resuscitation (1) Take 10ml of organ like passage culture buffer G and place it in a 15ml centrifuge tube<(2) Remove frozen organoid cells from a liquid nitrogen tank and quickly melt them in a 37 ℃ water bath(5) Suspension of matrix adhesive, with 25 ul of matrix adhesive per well laid on a 24 well cell culture plate, placed in a culture chamber for 10-15 minutes to form gel, and 500 ul of porcine endometrial organoid culture medium A. |
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| Description | Composition:
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| Storage Temp. | 4 ℃, 3months; -20 ℃, 1 year, see the label for details |
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4.9 ★★★★★
Based on 22 reviews
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Product Reviews
★★★★★ 5
soft, cozy, lightweight. Drapes nicely or folds flat & even. Warm as liner to another blanket
Color: Orange, Size: Twin(60"x80"), Color: Orange, Size: Twin(60"x80")
Summer weight, soft feel, cozy. If using in cooler temps, then consider using it as a liner, then toss another blanket on top. Nice cozy way to trap in heat.
I love orange and was looking for a functional throw blanket. I didnt want the typical lap blanket size (50"x60"). I am tall and wanted something I can tuck my feet under and pull up to my chest when on the couch. But I also wanted it to be decorative when not in use. Not wanting a thick blanket for this purpose, I looked for a thin one of this style. It folds small and flat, OR drapes nicely when tossed. Even at twin size (60"x 80"), it still works as a "throw" blanket. It measures a little wider and aittle shorter so not perfect twin length.
It is thin, but not see through. Very soft. Haven't washed it yet, so hopefully it holds up.
ALSO, we have cats and dogs, so I am always looking at something to see if it can also be used for them. Depending on size, these blankets would make good furniture covers. Nicer than sheets, cheaper than pet products, thin enough to toss in washer, and a material that will stay in place on fabric furniture. Or just a blankey for pets that like to get cozy.
WHICH, at the time of my writing this, my recent rescue dog from the south is experiencing the cool fall temps of fall. She is snuggled up tight against me. I have this blanket as the base on me and the couch, and then a thicker fleece blanket on top of me and her. I just took a pic and will include it.
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Reviewed in the United States on October 11, 2023
★★★★★ 5
Comfortable, durable and great for everyday use
Color: Brown, Size: Queen(90"x90")
This blanket is super cozy and soft, and it’s true to size. I got the brown color, and the quality is excellent. I’ve washed it multiple times, and it comes out looking like new every time.
It’s on the thinner side but still keeps you warm—perfect for lounging on the couch or sitting by the fire. The fabric stretches a bit, but not in a way that would cause it to rip or break.
Pros:
Soft and cozy
True to size
Maintains quality after washing
Lightweight yet warm
Slight stretch without damage
Cons:
Thin, may not be sufficient for very cold nights
Conclusion:
Overall, this blanket is comfortable, durable, and great for everyday use. It’s ideal for relaxing at home while staying warm.
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Reviewed in the United States on January 1, 2026
★★★★★ 5
Luxuriously soft
Color: Dark Brown, Size: Throw(50"x60")
So well made, looks great. So soft and light weight yet very warm.
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Reviewed in the United States on May 7, 2026
★★★★★ 5
Nice Lightweight Blanket
Color: Light Camel, Size: King(108"x90")
I bought this because my winter blanket is too heavy for the summer. I'm a hot sleeper and have to have the temperature at 68 at night because the bedroom is upstairs above a garage and it gets warm. This blanket is very soft and not thick and I don't get hot. I have a queen size bed but I bought a king size so it looks more like a bedspread. It doesn't hang down far on one side but that is the side that people don't see so that's fine. If you like a blanket on the thinner side yet still warm enough, you will like this.
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Reviewed in the United States on May 19, 2026
★★★★★ 4
Bright color & true to size
Color: Red, Size: Twin(60"x80")
I ordered red. Color was vibrant although it has a touch of Orange - not the deep red I thought I would get. Funky chemical smell when I opened it - it went away after I spread it out for a few hours. It's soft with hemmed edges. A little thin but OK for the bed I ordered it for. Price was right & I would probably order additional colors if warranted in the future. It arrived quick.
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Reviewed in the United States on January 10, 2026
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