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Description
DAF/CD55 Mouse mAb (S-628-1)Product Specification Host Mouse Antigen DAF CD55 Synonyms CR Immunogen Recombinant Protein Location Cell membrane Accession P08174 Clone Number S 628 1 Antibody Type Mouse mAb Isotype IgG1,k Application WB, FCM Reactivity Hu Purification Protein G Concentration 2 mg ml Conjugation Unconjugated Physical Appearance Liquid Storage Buffer PBS, 40% Glycerol, 0. 05%BSA, 0. 03% Proclin 300 Stability & Storage 12 months from date of receipt reconstitution,
Product Specification
| Host | Mouse |
| Antigen | DAF/CD55 |
| Synonyms | CR |
| Immunogen | Recombinant Protein |
| Location | Cell membrane |
| Accession | P08174 |
| Clone Number | S-628-1 |
| Antibody Type | Mouse mAb |
| Isotype | IgG1,k |
| Application | WB, FCM |
| Reactivity | Hu |
| Purification | Protein G |
| Concentration | 2 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05%BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| WB | 1:1000 | |
| FCM | 1:200 |
Background
DAF regulates the complement system on the cell surface. It recognizes C4b and C3b fragments that are created during activation of C4 (classical or lectin pathway) or C3 (alternative pathway). Interaction of DAF with cell-associated C4b of the classical and lectin pathways interferes with the conversion of C2 to C2b, thereby preventing formation of the C4b2a C3-convertase, and interaction of DAF with C3b of the alternative pathway interferes with the conversion of factor B to Bb by factor D, thereby preventing formation of the C3bBb C3 convertase of the alternative pathway. Thus, by limiting the amplification convertases of the complement cascade, DAF indirectly blocks the formation of the membrane attack complex.
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