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Description
HB-EGF Protein, HumanProduct Specification Species Human Synonyms Proheparin binding EGF like growth factor (HB EGF),Heparin binding EGF like Growth Factor,DT R Accession Q99075 Amino Acid Sequence Asp63 Leu148 Expression System E. coli Molecular Weight Approximately 9. 7 kDa, a single non glycosylated polypeptide chain containing 86 amino acids. Purity 97% by SDS PAGE or HPLC. Conjugation Unconjugated Tag No Tag Physical Appearance Lyophilized Powder Storage Buffer 10 mM
Product Specification
| Species | Human |
| Synonyms | Proheparin-binding EGF-like growth factor (HB-EGF),Heparin-binding EGF-like Growth Factor,DT-R |
| Accession | Q99075 |
| Amino Acid Sequence | Asp63-Leu148 |
| Expression System | E.coli |
| Molecular Weight | Approximately 9.7 kDa, a single non-glycosylated polypeptide chain containing 86 amino acids. |
| Purity | ＞97% by SDS-PAGE or HPLC. |
| Conjugation | Unconjugated |
| Tag | No Tag |
| Physical Appearance | Lyophilized Powder |
| Storage Buffer | 10 mM PB, with 500 mM NaCl, 5% (v/v) Trehalose, pH 7.4. |
| Reconstitution | Before use this product, please read the direction below carefully. This vial must be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in a sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/ml. Stock solutions should be apportioned into working aliquots and stored at ≤ -20℃. Further dilutions should be made in appropriate buffered solutions. |
| Stability & Storage | For long term storage, the product should be stored ≤ -20℃. |
Background
Heparin-binding epidermal growth factor (HB-EGF) -like growth factor (EGF) is found in cerebral neurons. Its expression is increased after hypoxic or ischemic injury, which also stimulates neurogenesis. HB-EGF has been implicated as a participant in a variety of normal physiological processes such as blastocyst implantation, wound healing, and in pathological processes such as tumor growth, SMC hyperplasia and atherosclerosis. HB-EGF is an 87 amino acid mitogenic and chemotactic glycoprotein containing an EGF-like domain with six conserved cysteine residues. Human HB-EGF shares about 73% and 76% a.a. sequence identity with murine and rat HB-EGF. Recombinant human HB-EGF is a 9.7kDa protein containing 86 amino acid residues.
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