Human FSH ELISA Kit

Detection Principle:

This kit uses double antibody sandwich enzyme-linked immunosorbent assay technology. Specific anti human fsh  The antibody was pre coated on a high affinity microplate. Add the standard and the sample to be tested into the wells of the enzyme plate, and after incubation, the FSH in the sample; Binding to solid-phase antibodies. After washing to remove unbound material, biotinylated detection antibody was added for incubation. Unbound biotinylated antibodies were removed by washing, and horseradish peroxidase labeled streptavidin HRP was added. After washing, the chromogenic substrate TMB was added to avoid light for color development. The depth of color response and fsh  Is proportional to the concentration of. The reaction was stopped by adding a stop solution, and the absorbance value was measured at a wavelength of 450 nm (reference wavelength 570 - 630 nm)

Detection Type: Double antibody sandwich method

Form: Pre coated 96 well plate

Test Sample Type: cell supernatant, serum, plasma

Loading Amount: 100 μ L

Kit Components: a copy of pre coated 96 well plate, standard, FSH detection antibody, standard dilution, detection buffer, TMB chromogenic substrate, washing solution, termination solution, sa-hrp, plate sealing membrane and instructions

Sensitivity: 0.36 miu/ml

Detection Range: 1.56-100miu/ml

Recovery Fange: 82-119%

Storage Method: 2-8 ℃

Standard Curve:

Background:

Follicle stimulating hormone (FSH, also known as follicle stimulating hormone) is a member of the glycoprotein hormone family. The members of this family are composed of the same α subunit and a hormone specific β subunit. Its structure is similar to luteinizing hormone (LH), thyroid stimulating hormone (TSH)And human chorionic gonadotropin (hCG). Alternative splicing generates two transcript variants encoding the same protein. FSH is synthesized and secreted by the gonadotropic cells of the anterior pituitary, and regulates the development, growth, pubertal maturation and reproductive process of the body. FSH and luteinizing hormone (LH) act together on the reproductive system. In males, FSH induces Sertoli cells to secrete androgen binding proteins (ABPs), which are involved in the regulation of the negative feedback mechanism of inhibin on the anterior pituitary. In particular, FSH activated Sertoli cells can maintain spermatogenesis and stimulate the secretion of inhibin B. In females, FSH initiates follicular growth, particularly affecting granulosa cells. With the increase of inhibin B, FSH levels will decrease in the late follicular phase.