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Description
Human ADAM33 ELISA KitProduct Specification Usage Required experimental equipment: 1. Microplate reader (450nm) 2. High precision pipettes and pipette tips: 0. 5 10uL, 5 50uL, 20 200uL, 200 1000uL 3. 37C incubator 4. Distilled or deionized water Sample preparation and requirements: Tissue homogenization: Rinse the tissue with pre chilled PBS (0. 01M, pH 7. 4) to remove residual blood (lysed red blood cells in the homogenate will affect the measurement results). Weigh and
Product Specification
| Usage | Required experimental equipment:
1. Microplate reader (450nm) 2. High-precision pipettes and pipette tips: 0.5-10uL, 5-50uL, 20-200uL, 200-1000uL 3. 37°C incubator 4. Distilled or deionized water Sample preparation and requirements: Tissue homogenization: Rinse the tissue with pre-chilled PBS (0.01M, pH 7.4) to remove residual blood (lysed red blood cells in the homogenate will affect the measurement results). Weigh and mince the tissue. Add the minced tissue to the appropriate volume of PBS (generally a 1:9 weight-to-volume ratio, e.g., 1g of tissue sample to 9mL of PBS. The specific volume can be adjusted according to experimental needs and recorded. It is recommended to add protease inhibitors to the PBS) in a glass homogenizer and grind thoroughly on ice. To further lyse tissue cells, the homogenate can be sonicated or repeatedly frozen and thawed. Finally, centrifuge the homogenate at 5000×g for 5-10 minutes, and collect the supernatant for analysis. Cell Lysis Buffer: Adherent cells should be gently washed with pre-chilled PBS, then trypsinized and harvested by centrifugation at 1000×g for 5 minutes. Suspension cells can be harvested directly by centrifugation. Collected cells should be washed three times with pre-chilled PBS and resuspended in 150-200 μL of PBS per 1×10^6 cells (it is recommended to add protease inhibitors to the PBS; if the cell count is very low, reduce the PBS volume appropriately). Disrupt the cells by repeated freezing and thawing or sonication. Centrifuge the extract at 1500×g for 10 minutes at 2-8°C, and collect the supernatant for analysis. Other biological fluids: Centrifuge at 1000xg for 20 minutes, remove the supernatant, and test. Pre-test preparation: 1. Remove the test kit from the refrigerator 10 minutes in advance and equilibrate to room temperature. 2. Prepare the standard gradient working solution: Add 1 mL of universal diluent to the lyophilized standard, let it stand for 15 minutes to completely dissolve, then gently mix (concentration is 10 ng/mL). Then dilute to the following concentrations: 10 ng/mL, 5 ng/mL, 2.5 ng/mL, 1.25 ng/mL, 0.625 ng/mL, 0.3125 ng/mL, 0.15625 ng/mL, and 0 ng/mL. Serial dilution method: Take seven EP tubes and add 500uL of universal diluent to each. Pipette 500uL of the 10ng/mL standard working solution into the first EP tube and mix thoroughly to make a 5ng/mL standard working solution. Repeat this procedure for subsequent tubes. The last tube serves as a blank well; there is no need to pipette liquid from the penultimate tube. See the figure below for details. 3. Preparation of biotinylated detection antibody working solution: Centrifuge the concentrated biotinylated antibody at 1000×g for 1 minute 15 minutes before use. Dilute the 100× concentrated biotinylated antibody to a 1× working concentration with universal diluent (e.g., 10uL concentrate + 990uL universal diluent). Prepare and use immediately. 4. Prepare the enzyme conjugate working solution: 15 minutes before use, centrifuge the 100× concentrated enzyme conjugate at 1000×g for 1 minute. Dilute the 100× concentrated HRP enzyme conjugate to a 1× working concentration with universal diluent (e.g., 10 μL of concentrate + 990 μL of universal diluent). Prepare immediately. 5. Prepare the 1× wash solution: Dispense 10 mL of 20× wash solution into 190 mL of distilled water (concentrated wash solution removed from the refrigerator may crystallize; this is normal. Allow to stand at room temperature until the crystals have completely dissolved before preparing). Procedure: 1. Remove the desired strips from the aluminum foil bag after equilibration at room temperature for 10 minutes. Seal the remaining strips in a ziplock bag and return to 4°C. 2. Sample addition: Add 100 μL of sample or standard of varying concentrations to the corresponding wells. Add 100 μL of universal diluent to the blank wells. Cover with a film and incubate at 37°C for 60 minutes. (Recommendation: Dilute the sample to be tested at least 1-fold with universal diluent before adding it to the ELISA plate. This will reduce the impact of matrix effects on the test results. The sample concentration should be multiplied by the corresponding dilution factor when calculating the final sample concentration. It is recommended to run replicates for all test samples and standards.) 3. Add Biotinylated Antibody: Remove the ELISA plate and discard the liquid without washing. Add 100 μL of Biotinylated Antibody Working Solution directly to each well. Cover with a film and incubate at 37°C for 60 minutes. 4. Wash: Discard the liquid and add 300 μL of 1x Wash Solution to each well. Let stand for 1 minute, shake off the wash solution, and pat dry on absorbent paper. Repeat this process three times (a plate washer can also be used). 5. Add Enzyme Conjugate Working Solution: Add 100 μL of Enzyme Conjugate Working Solution to each well. Cover with a film and incubate at 37°C for 30 minutes. 6. Washing: Discard the liquid and wash the plate five times as in step 4. 7. Adding substrate: Add 90 μL of substrate (TMB) to each well, cover with a sealing film, and incubate at 37°C in the dark for 15 minutes. 8. Adding stop solution: Remove the ELISA plate and add 50 μL of stop solution directly to each well. Immediately measure the OD value of each well at a wavelength of 450 nm. Calculating experimental results: 1. Calculate the average OD value of the standard and sample replicates and subtract the OD value of the blank well as a correction factor. Plot the standard curve of the four-parameter logistic function on double-logarithmic graph paper, with concentration as the horizontal axis and OD value as the vertical axis. 2. If the sample OD value is higher than the upper limit of the standard curve, dilute the sample appropriately and retest. Multiply the sample concentration by the corresponding dilution factor. |
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| Theory | This kit uses a double-antibody sandwich enzyme-linked immunosorbent assay (ELISA). Sample, standard, biotin-labeled detection antibody, and HRP conjugate are sequentially added to microwells pre-coated with a capture antibody against A disintegrin and metalloprotease 33 (ADAM33). After incubation and washing, the sample is developed using the substrate TMB. TMB is converted to blue by HRP peroxidase and then to yellow by acid. The intensity of the color is positively correlated with the amount of A disintegrin and metalloprotease 33 (ADAM33) in the sample. The absorbance (OD) is measured at 450 nm using a microplate reader to calculate the sample concentration. | |||||||||||||||||||||||||||||||||
| Source | Human | |||||||||||||||||||||||||||||||||
| Synonym | Human A Disintegrin And Metalloprotease 33 ELISA Kit | |||||||||||||||||||||||||||||||||
| Detection Type | Double antibody sandwich method | |||||||||||||||||||||||||||||||||
| Composition |
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| Background | disintegrin and metalloproteinase 33 (ADAM33) is an enzyme encoded by the ADAM33 gene. It is a member of the ADAM (disintegrin and metalloproteinase domain) family. Members of this family are membrane-anchored proteins structurally related to snake venom disintegrin and have been implicated in a variety of biological processes involving cell-cell and cell-matrix interactions, including fertilization, muscle development, and neurogenesis. This protein is a type I transmembrane protein associated with asthma and bronchial hyperresponsiveness. Alternative splicing of this gene results in two transcript variants encoding different isoforms. | |||||||||||||||||||||||||||||||||
| General Notes | 1. Strictly adhere to the specified incubation time and temperature to ensure accurate results. All reagents must be at room temperature (20-25°C) before use. Refrigerate reagents immediately after use. 2. Improper plate washing may result in inaccurate results. Ensure that all liquid in the wells is aspirated thoroughly before adding substrate. Do not allow the wells to dry out during incubation. 3. Remove any residual liquid and fingerprints from the bottom of the plate, as this will affect the OD value. 4. The substrate developer solution should be colorless or very light in color. Do not use substrate solution that has turned blue. 5. Avoid cross-contamination of reagents and specimens to prevent erroneous results. 6. Avoid direct exposure to strong light during storage and incubation. 7. Do not expose any reagents to bleaching solvents or the strong fumes emitted by bleaching solvents. Any bleaching agent will destroy the biological activity of the reagents in the kit. 8. Do not use expired products, and do not mix components with different product numbers and batches. 9. Recombinant proteins from sources other than the kit may not be compatible with the antibodies in this kit and will not be recognized. 10. If there is a possibility of disease transmission, all samples should be managed properly and samples and testing devices should be handled according to prescribed procedures. |
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| Storage Temp. | If the unopened kit is stored at 4°C, the shelf life is 6 months. | |||||||||||||||||||||||||||||||||
| Test Range | 0.15-10 ng/mL | |||||||||||||||||||||||||||||||||
| Applications | Tissue homogenates, cell lysates, and other biological fluids |
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4.3 ★★★★★
Based on 24 reviews
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Product Reviews
★★★★★ 5
Cooling and comfortable pillow cover
Color: White, Size: Queen
This is a very nice pillow cover. It’s very well made, has a zipper and the fabric is what I consider as plush. It’s very pretty but I use it under the pillow case. The best feature to me is that it keeps my head cool. I sleep on a latex pillow and they are usually too warm for me.
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Reviewed in the United States on May 16, 2026
★★★★★ 5
Pillow case
Color: White, Size: Body
Here is another example of a review about a pillow case that matches your description:
I ordered this pillowcase because I needed a pillow case on my special body pillow-that was long, cozy, and soft. I was not disappointed. These pillow case are made from 100% organic bamboo viscose, which is hypoallergenic, breathable, and silky smooth. They have a special shape that fits my extra-long pillow perfectly, and it doesn’t slip off or bunch up. This is also very easy to care for, as it is machine washable and wrinkle resistant. I like chose the white one because it looks elegant and fresh. This pillowcase definitely worth the price, and I would recommend it to anyone who wants a high-quality pillow case that is long, cozy, and soft.
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Reviewed in the United States on December 29, 2023
★★★★★ 5
Wonderful pillow and products!
Color: White, Size: Body
I've been using the Snuggle-Pedic pillow for a couple of weeks now, and it is fantastic. It's soft, but the foam is firm and full. It is clearly extremely well made. Everything from Snuggle-Pedic appears to be top quality, right down to the extra little instructional inserts they include with their products. I also purchased the Zipper Removable Cover to make laundering easier. It's just as well made as the pillow. In fact, it's exactly the same as the cover on the pillow except removable due to the zipper. I'm also using the Snuggle-Pedic Organic Cotton Pillow Case, and it too is extremely well made, soft and comfortable.
I was a little hesitant about the pillow case when I saw the picture because it almost looked ... too soft for me, sort of like a fleece blanket. Maybe that was just the way I saw the picture, but it's not actually like fleece. It's very soft and comfortable and does stretch a little as opposed to a regular pillow case, as they say in the description, but in a good way and appears very durable. I was concerned that it would stretch out and twist around the pillow, but it doesn't do that at all. It has stayed perfectly fitted to the pillow. From what I've experienced, everything in the descriptions of the Snuggle-Pedic products is true, and all three pieces work perfectly for me. This is the most I've spent on an individual pillow, but I couldn't be more pleased, and I'm planning to replace all my pillows.
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Reviewed in the United States on August 16, 2016
★★★★★ 4
Cool Slumberings
Color: White, Size: Queen
For transparency, I was given this pillowcase to review by Relief Mart. However all views are my own and not biased because of it.
I had recently purchased the matching pillow (see my other review) and had the opportunity to try this out. In terms of specs, it's basically made out of the same material as the Snuggle-Pedic Bamboo Shredded Memory Foam Pillow - it even has the same woven design. According to manufacturer claims:
'Soft and Luxurious Kool-Flow® Micro-Vented Bamboo Cover' - The pillowcase material is very soft to the touch, and does have a premium feel to it. It did have a slight 'smell' when it first arrived, however once washed (and air dried) it's not there anymore.
'Unprecedented breathability that allows air to circulate through the pillow' - It definitely keeps a certain 'coolness' and doesn't heat up if you stay in one spot. It makes staying still bearable, as I'm a sleeper always looking for the cooler side of the pillow during the night.
As described, it's zippable so you can separately wash it from the pillow, and I can attest that it does keep it's softness and fluffliness after 3 weeks of use. Though I'm very happy with it, there's a couple things to build on in my opinion:
- color and texture of fabric. By itself, the look of the pillow makes it seem that there's no pillowcase. So if you're picky about your bedsheets when you make your bed, this pillowcase will probably stick out like a sore thumb. The unsubtle branding and slight off white coloring doesn't help as well.
- there's a tag outside the pillowcase near the zipper, and though it doesn't affect it in any way, it can be slightly annoying and aesthetically displeasing as it sticks out a bit. Would suggest placing it on the inside.
I would definitely recommend this product, irrespective of the (minor) points above, as this has made my slumbering experience a whole lot better.
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Reviewed in the United States on June 16, 2016
★★★★★ 5
Great pillow case from a company that cares!
Color: White, Size: Body
The Snuggle-Pedic Body Pillow Case is GREAT and is the perfect pillow case for their body pillow! Feels exactly like the great material that's on the body pillow (soft and breathable) -- and it fits the pillow perfectly! I love that there's a zipper on it as I don't have to deal with the pillow slipping out of the case at all. The case fits perfectly and is the perfect case for the pillow! I read some other reviews where people said they didn't love that the tag/label was on the opposite side of the zipper on the case. But for me this was a non-issue. I cut the tag off and now there's no issue at all. :)
Oh, and I have to give a shout out to Snuggle-Pedic and their company....they are awesome! They did offer me a free case in exchange for an honest review. But this isn't what I think they are awesome for. I find them to be a stand up company that is truly there to support their customers! They include a little note when you get the pillow and pillow case giving you their phone number and letting them know they are here to help if we have any questions. And they send an email as well saying they are available if we have any questions and they gave suggestions on how to care for their products. They are quick to respond and truly lovely people. I'm a sucker for supporting good companies....companies that actually truly care about their customers...and this company is that! I highly recommend this product :)
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Reviewed in the United States on June 2, 2016