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Description
Alexa Fluor® 647 Mouse Anti-Human IgE Antibody (S-2853)Product Specification Host Mouse Antigen IgE Synonyms Immunoglobulin heavy constant epsilon; Ig epsilon chain C region; Ig epsilon chain C region ND; IGHE Location Secreted, Cell membrane Accession P01854 Clone Number S 2853 Antibody Type Mouse mAb Isotype IgG1,k Application FCM Reactivity Hu Positive Sample Human PBMC Purification Protein G Concentration 0. 2 mg ml Conjugation Alexa Fluor 647 Physical Appearance Liquid Storage Buffer PBS, 1% BSA, 0.
Product Specification
| Host | Mouse |
| Antigen | IgE |
| Synonyms | Immunoglobulin heavy constant epsilon; Ig epsilon chain C region; Ig epsilon chain C region ND; IGHE |
| Location | Secreted, Cell membrane |
| Accession | P01854 |
| Clone Number | S-2853 |
| Antibody Type | Mouse mAb |
| Isotype | IgG1,k |
| Application | FCM |
| Reactivity | Hu |
| Positive Sample | Human PBMC |
| Purification | Protein G |
| Concentration | 0.2 mg/ml |
| Conjugation | Alexa Fluor® 647 |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 1% BSA, 0.3% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, 2 to 8 °C as supplied |
Dilution
| application | dilution | species |
| FCM | 5μl per million cells in 100μl volume | Hu |
Background
IgE is an antibody isotype that, despite being the least abundant in serum, sits at the epicenter of type I hypersensitivity and anti-parasite immunity; generated by plasma cells in mucosal tissues, it binds with picomolar affinity to the tetrameric FcεRI on mast cells and basophils, priming them for rapid degranulation upon subsequent allergen encounter, thereby unleashing histamine, leukotrienes and cytokines that produce the clinical spectrum from urticaria to anaphylaxis, while also orchestrating eosinophil and macrophage responses against helminths; its half-life in plasma is brief (~2 days), yet it can remain cell-bound for weeks, and its levels are tightly regulated by IL-4, IL-13 and CD40L-mediated class-switching.
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4.0 ★★★★★
Based on 7 reviews
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Product Reviews
★★★★★ 3
Liked it
Format: Kindle
I liked the worlds and the characters. The prophesy is confusing in that I would think, since all worlds ore on a path to destruction, the angels would want their world to be saved. I am sure there is more evil that will be revealed as the series proceeds. I dislike a prolonged “poor little me” trope and the main character has moved past that, thankfully, and began to show a fierce attitude as the novel progressed. The last portion of the novel had some great action. It’s worth reading, just be patient with a slow start.
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Reviewed in the United States on February 14, 2024
★★★★★ 5
A Fox Amongst the Wolves
Format: Kindle
Collette has been forgotten about her whole life. Her parents don't bother with her, others don't try either once they realize she uses sign language to speak, and she's a shifter surrounded by humans that hate her kind. On her 18th birthday, she runs away. She takes the train and then bus wherever it can get her as far from home as possible. A missed bus brings her and a mysterious but kind man to Willowdale. What happens next is a story of fated mates, shifters, magic, new best friends and found family, and a stolen child. I'm sad this was a shorter read but only because of how much I loved Collette, Dylan, Hunter, Luca, Lena, and the others in their community. I love how detailed the characters are even with the shorter story. You can tell their characteristics easily, and the lead in to book two is done so well. I loved this insta love book with just the right amount of drama.
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Reviewed in the United States on July 10, 2025
★★★★★ 4
An easy PNR book to cure book hangovers.....
Format: Kindle
I enjoyed this as a short story; but nothing truly pulled me into this world to want to keep reading each story. I can sense the overarching villain arc w/ the HAF; yet it still wasn't enough to capture me long-term. It felt like too much focus was placed on progressing the romance part that everything else took a non-existent back seat. This did work well for my intentions of getting over a book-hangover slump. So there's that lol. A Tip to overcome a PNR book hangover, from a wonderful series or novel......read a trope-y, lighthearted, PNR that is easily predictable but adorable. Like this book.
Honestly, I'm rarely into short stories that are turned into a ridiculous amount of books on different PNR characters. So I'm not the best at judging the merits of these stories. I'm a long-haul type of reader that lives for a series surrounding 1 lead female character & her harem (or singular partner). Occassionally, I'll find a series of short stories that are interesting enough to finish or pull me in completely. The rarity of that is countable on 1.5 hands, compared to the literal hundreds of fiction books I've read lol.
With all of that, I will say this was fun because of the FMC's difficulties growing up & how that made her strong, the end reveals of her life, & the cute Epilogues. I never felt truly connected to any of the characters; but I did feel slightly compelled to just finish it to see how plot points would end.
Unfortunately, due to a lack of character backstory explanations, I still don't understand Collete's "magic". Very little time was given to flesh out the backstory of the world this occurs in. I couldn't even guess what type of magic occurs in this world, aside from what little was revealed. A random scene sticks out to me...... we're introduced to a new magic type, in passing. No dialogue to expand. No hint at anything really. Then the scene moves on. That moment felt pointless to even mention, afterwards.
It felt like the author was more concerned w/ romance than storyline. This is a common issue I have w/ short stories....authors write them for whatever reason; but too often it's an "either or" theme.....EITHER the author is writing ONLY for romance w/ storyline taking a minor role OR writing ONLY for storyline w/ some romance that ends up feeling forced. As I mentioned earlier, only a handful of short stories series have ever had a balance of both sides, for me, that I wanted to read all of them.
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Reviewed in the United States on May 16, 2021
★★★★★ 5
Great RH shapeshifting romance!
Format: Kindle
Oh my gosh, I adored this book! Voiceless is set in a blended reality with humans, witches, and shapeshifters of all kinds, but with a significant lack of acceptance from the humans that results in violence. And I am sucker for fated-mates/insta-connection and you get that in heaps and bounds in Voiceless! Colette’s introduction to the supernatural world added some challenges on top of not being able to effectively communicate with those around her. I enjoyed the overall plot; that there was danger and confrontation and resolution, albeit not nearly as much as I wanted. Voiceless is a short read and things escalate at a fast pace and a resolution is reached just as quickly. As a result, I think we miss out a bit on relationship and character development that we could’ve had if the book was little longer. While this is a RH/why-choose, most, if not all of the spice, is exclusively MF.
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Reviewed in the United States on March 17, 2025
★★★★★ 3
Cute
Format: Kindle
Very insta-love, but also cute with likable characters. The plot was good, though lacking in depth. The spice was fairly abrupt and lack-luster.
Am enjoyable read, overall.
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Reviewed in the United States on May 22, 2026