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Description
Human GLRX3 ELISA KitProduct Specification Usage Required experimental equipment: 1. Microplate reader (450nm) 2. High precision pipettes and pipette tips: 0. 5 10uL, 5 50uL, 20 200uL, 200 1000uL 3. 37C incubator 4. Distilled or deionized water Sample handling and requirements: Plasma: Collect the specimen using EDTA or heparin as an anticoagulant. Centrifuge at 1000g for 15 minutes at 2 8C within 30 minutes of collection. Remove the supernatant for testing or store at
Product Specification
| Usage | Required experimental equipment: 1. Microplate reader (450nm) 2. High-precision pipettes and pipette tips: 0.5-10uL, 5-50uL, 20-200uL, 200-1000uL 3. 37°C incubator 4. Distilled or deionized water Sample handling and requirements: Plasma: Collect the specimen using EDTA or heparin as an anticoagulant. Centrifuge at 1000×g for 15 minutes at 2-8°C within 30 minutes of collection. Remove the supernatant for testing or store at -20°C or -80°C, but avoid repeated freezing and thawing. Pre-test preparation: 1. Remove the test kit from the refrigerator 10 minutes in advance and equilibrate to room temperature. 2. Prepare a gradient standard working solution: Add 1 mL of universal diluent to the lyophilized standard. Let stand for 15 minutes to completely dissolve, then gently mix (concentration 10 ng/mL). Then dilute to the following concentrations: 10 ng/mL, 5 ng/mL, 2.5 ng/mL, 1.25 ng/mL, 0.625 ng/mL, 0.3125 ng/mL, 0.15625 ng/mL, and 0 ng/mL. Serial dilution method: Take seven EP tubes and add 500 μL of universal diluent to each tube. Pipette 500 μL of the 10 ng/mL standard working solution into the first EP tube and mix thoroughly to make a 5 ng/mL standard working solution. Repeat this procedure for subsequent tubes. The last tube serves as a blank well; there is no need to pipette liquid from the penultimate tube. See the figure below for details. 3. Preparation of biotinylated detection antibody working solution: Centrifuge the concentrated biotinylated antibody at 1000×g for 1 minute 15 minutes before use. Dilute the 100× concentrated biotinylated antibody to a 1× working concentration with universal diluent (e.g., 10uL concentrate + 990uL universal diluent). Prepare and use immediately. 4. Prepare the enzyme conjugate working solution: 15 minutes before use, centrifuge the 100× concentrated enzyme conjugate at 1000×g for 1 minute. Dilute the 100× concentrated HRP enzyme conjugate to a 1× working concentration with universal diluent (e.g., 10 μL of concentrate + 990 μL of universal diluent). Prepare immediately. 5. Prepare the 1× wash solution: Dispense 10 mL of 20× wash solution into 190 mL of distilled water (concentrated wash solution removed from the refrigerator may crystallize; this is normal. Allow to stand at room temperature until the crystals have completely dissolved before preparing). Procedure: 1. Remove the desired strips from the aluminum foil bag after equilibration at room temperature for 10 minutes. Seal the remaining strips in a ziplock bag and return to 4°C. 2. Sample addition: Add 100 μL of sample or standard of varying concentrations to the corresponding wells. Add 100 μL of universal diluent to the blank wells. Cover with a film and incubate at 37°C for 60 minutes. (Recommendation: Dilute the sample to be tested at least 1-fold with universal diluent before adding it to the ELISA plate. This will reduce the impact of matrix effects on the test results. The sample concentration should be multiplied by the corresponding dilution factor when calculating the final sample concentration. It is recommended to run replicates for all test samples and standards.) 3. Add Biotinylated Antibody: Remove the ELISA plate and discard the liquid without washing. Add 100 μL of Biotinylated Antibody Working Solution directly to each well. Cover with a film and incubate at 37°C for 60 minutes. 4. Wash: Discard the liquid and add 300 μL of 1x Wash Solution to each well. Let stand for 1 minute, shake off the wash solution, and pat dry on absorbent paper. Repeat this process three times (a plate washer can also be used). 5. Add Enzyme Conjugate Working Solution: Add 100 μL of Enzyme Conjugate Working Solution to each well. Cover with a film and incubate at 37°C for 30 minutes. 6. Washing: Discard the liquid and wash the plate five times as in step 4. 7. Adding substrate: Add 90 μL of substrate (TMB) to each well, cover with a sealing film, and incubate at 37°C in the dark for 15 minutes. 8. Adding stop solution: Remove the ELISA plate and add 50 μL of stop solution directly to each well. Immediately measure the OD value of each well at a wavelength of 450 nm. Calculating experimental results: 1. Calculate the average OD value of the standard and sample replicates and subtract the OD value of the blank well as a correction factor. Plot the standard curve of the four-parameter logistic function on double-logarithmic graph paper, with concentration as the horizontal axis and OD value as the vertical axis. 2. If the sample OD value is higher than the upper limit of the standard curve, dilute the sample appropriately and retest. Multiply the sample concentration by the corresponding dilution factor. |
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| Theory | This kit uses a double-antibody sandwich enzyme-linked immunosorbent assay (ELISA). Sample, standard, biotin-labeled detection antibody, and HRP conjugate are sequentially added to microwells pre-coated with a glutaredoxin 3 (GLRX3) capture antibody. After incubation and washing, the sample is developed using the substrate TMB. TMB is converted to blue by HRP peroxidase and to yellow by acid. The intensity of the color is positively correlated with the amount of glutaredoxin 3 (GLRX3) in the sample. The absorbance (OD) is measured at 450 nm using a microplate reader to calculate the sample concentration. | |||||||||||||||||||||||||||||||||
| Source | Human | |||||||||||||||||||||||||||||||||
| Synonym | Human Glutaredoxin 3 ELISA Kit | |||||||||||||||||||||||||||||||||
| Detection Type | Double antibody sandwich method | |||||||||||||||||||||||||||||||||
| Composition |
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| Background | Glutaredoxin 3 (GLRX3), also known as thioredoxin-like protein 2 (TXNL2), is a protein encoded by the GLRX3 gene. This gene encodes a member of the glutaredoxin family. The encoded protein binds to and regulates protein kinase C theta. It may also inhibit apoptosis and play a role in cell growth, and its expression may be a marker for cancer. Its pseudogene is located on the short arms of chromosomes 6 and 9. Alternative splice transcript variants of this gene have been observed. Together with BOLA2, it functions as a cytosolic iron-sulfur (Fe-S) cluster assembly factor, promoting the insertion of [2Fe-2S] clusters into a subset of cytosolic proteins. It acts as a key negative regulator of cardiac hypertrophy and a positive inotropic regulator. It is required for hemoglobin maturation. It does not possess any thyroredoxin activity because it lacks a conserved motif required for catalytic activity. It has been shown to interact with PRKCQ. | |||||||||||||||||||||||||||||||||
| General Notes | 1. Strictly adhere to the specified incubation time and temperature to ensure accurate results. All reagents must be at room temperature (20-25°C) before use. Refrigerate reagents immediately after use. 2. Improper plate washing may result in inaccurate results. Ensure that all liquid in the wells is aspirated thoroughly before adding substrate. Do not allow the wells to dry out during incubation. 3. Remove any residual liquid and fingerprints from the bottom of the plate, as this will affect the OD value. 4. The substrate developer solution should be colorless or very light in color. Do not use substrate solution that has turned blue. 5. Avoid cross-contamination of reagents and specimens to prevent erroneous results. 6. Avoid direct exposure to strong light during storage and incubation. 7. Do not expose any reagents to bleaching solvents or the strong fumes emitted by bleaching solvents. Any bleaching agent will destroy the biological activity of the reagents in the kit. 8. Do not use expired products, and do not mix components with different product numbers and batches. 9. Recombinant proteins from sources other than the kit may not be compatible with the antibodies in this kit and will not be recognized. 10. If there is a possibility of disease transmission, all samples should be managed properly and samples and testing devices should be handled according to prescribed procedures. |
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| Storage Temp. | If the unopened kit is stored at 4°C, the shelf life is 6 months. | |||||||||||||||||||||||||||||||||
| Test Range | 0.156-10 ng/mL | |||||||||||||||||||||||||||||||||
| Applications | plasma |
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4.5 ★★★★★
Based on 16 reviews
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Product Reviews
★★★★★ 4
ALL SEASON IS TOO WARM!
Size: King, Style: All-Season
I got the King, All Season, to squeeze into a Queen duvet for extra floof and it's sooo cozy. Highly recommend.
I redacted 1 star though because although it says All Season, the amount of down in here feels like it's for winter. The first night I used it I woke up dripping in sweat. It's currently March, I live in Los Angeles area, hitting about 75° weather outside rn, but I sleep with AC full blast at night (usually hit about 64 when I wake up). I can't sleep with it, I'll need to buy the "cool" one, and hope that isn't tooo thin.
Don't get me wrong, it's a great insert, but holy hell, it's too heckin' warm. I saw someone else mention it and wish I had honed in on their comment. I don't wanna return it as it was used, so I'm stuck with it now.
*UPDATE*
I am back because I also bought the "Cool" blanket as well after experiencing the heat of the all season and it too is also too hot for me. I've had a duvet before but I guess not filled to the capacity of these blankets. If you're a hot sleeper maybe look elsewhere?
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Reviewed in the United States on March 12, 2026
★★★★★ 5
Buy this instead of the ‘brand name’ one
Size: Twin, Style: Warm
Tl:DR:
Buy it, it’s super cheap, better than the one I paid 2x the price for and soft as it can get, and it really is a ‘Winter comforter’.
This review is going to sound like I got paid off for it but I actually didn’t. I bought another comforter prior to this one, paid close to double of this one and that the ‘on sale’ price, and it is trash compared to this Amazon basics one. Both claim to be thick warm comforters but I can literally stack two of the other brand and maybe they’d be almost as thick as the Amazon one. I only bought this one because the other ‘winter’ one I bought was so thin and flimsy I figured adding another ‘cheapie’ one might make me not freeze to death. As soon as I arrived I noticed that this Amazon one was WAY thicker, nicer looking, softer and warmer than the old one. Just to try it out I stacked both of them together to see if I wouldn't freeze to death like I was doing with the old ‘expensive’ one: five minutes in and I was sweating. That’s all it took, I immediately removed the duvet cover from the old one, switched it to the Amazon one and threw the old one somewhere where I couldn’t see the money I had wasted, and yes the Amazon was warmer and softer even WITHOUT the duvet cover. I’m probably going to get the light version for when fall hits and I don’t quite need the heavy one yet. Buy it you won’t regret it and hey it’s Amazon brand so if you don’t like it they’ll take care of you, but I HIGHLY doubt you won’t love this thing.
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Reviewed in the United States on April 14, 2026
★★★★★ 5
Great duvet insert
Size: King, Style: Light
Perfect duvet insert. I got a pretty expensive duvet to cover this and they worked great together. The fit was perfect and I didn’t feel like the duvet was smaller than its cover. This was a cheap option that keep us warm while not being too think. This product is also breathable and recommend if you live in a place that transitions from hot to cold often.
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Reviewed in the United States on April 6, 2026
★★★★★ 5
Great mid-weight comforter
Size: King, Style: Light
Great comforter, especially for the price. I put it in the dryer for a bit when I first got it and it became nice and fluffy. Perfect mid-weight duvet.
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Reviewed in the United States on May 12, 2026
★★★★★ 5
Totally love them
Color: Dark Tortoise, Size: 55-18-140
Super cute a little bigger than I expected but still adorable and trendy!
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Reviewed in the United States on January 17, 2026