Pay in installments of $56.25 with
,
and
Shipping Estimate
USA
- USA
- CAN
- USA
- CAN
Ships within 48 hours · Estimated delivery Aug 17 - Aug 22
Promo Codes Available:
For Your Every Summer RSVP, with Code: SUMMER15
Description
Human vitamin D ELISA KitProduct Specification protein VD Usage Specimen requirements: 1 . The specimen should be extracted as soon as possible after collection, and the extraction should be carried out according to the relevant literature. Experiments should be carried out as soon as possible after extraction. If the test cannot be carried out immediately, the specimen can be placed in 20 Store, but avoid repeated freezing and thawing 2 . Unable to detect containing NaN3
Product Specification
| protein | VD | ||||||||||||||||||||||||
| Usage | Specimen requirements: 1 . The specimen should be extracted as soon as possible after collection, and the extraction should be carried out according to the relevant literature. Experiments should be carried out as soon as possible after extraction. If the test cannot be carried out immediately, the specimen can be placed in -20℃ Store, but avoid repeated freezing and thawing 2 . Unable to detect containing NaN3 NaN3 Inhibition of horseradish peroxidase (HRP) activity. Operation steps: 1. Dilution of standard: This kit provides one original standard, which can be diluted in a small test tube according to the following chart.
2. Sample addition: Blank wells (no sample and enzyme labeled reagent are added to the blank control wells, and the other steps are the same), standard wells and sample wells to be tested are set respectively. Accurate spike of standard on enzyme-labeled coated plate 50uL, first add sample diluent to the sample well to be tested 40uL, then add the sample to be tested 10uL (The final dilution of sample was 5 Times). Add the sample to the bottom of the well of the enzyme labeled plate, try not to touch the well wall, and gently shake and mix well. 3. Incubation: After plate sealing with plate sealing film 37℃ Incubation 30 Minutes. 4. Solution preparation: will 30 Distilled water for double concentrated washing liquid 30 Time dilution for use 5. Washing: Carefully remove the sealing film, discard the liquid, spin dry, fill each well with washing liquid, and let stand 30 Discard it in seconds and repeat it 5 Times, pat dry. 6. Enzyme addition: enzyme labeled reagent is added to each well 50uL, except for blank holes. 7. Incubation: Same procedure 3. 8. Washing: Same operation 5. 9. Color development: Add color developer to each well first A 50uL And then adding a color developer B 50uL, gently shake and mix evenly, 37℃ Color development protected from light 10 Minutes. 10. Stop: Add stop solution per well 50uL, terminate the reaction (blue immediately turns yellow at this time). 11. Measurement: zeroing with blank holes, 450nm The absorbance of each well was measured sequentially by wavelength (OD Value). The determination shall be performed after addition of the stop solution 15 Within minutes. Calculation: Taking the concentration of the standard as the abscissa, OD The value is the ordinate, draw a standard curve on the coordinate paper, and according to the sample OD The corresponding concentration is found out from the standard curve; Multiply by the dilution factor; Or use the concentration of the standard substance to OD The linear regression equation of the standard curve is calculated from the value, and the sample OD The value is substituted into the equation, the sample concentration is calculated, and then multiplied by the dilution factor, which is the actual concentration of the sample. |
||||||||||||||||||||||||
| Species Reactivity | Human | ||||||||||||||||||||||||
| Theory | This kit uses double antibody sandwich method to determine the level of human vitamin D in specimens. Coating a microwell plate with purified human vitamin D antibody to make a solid phase antibody, adding vitamin D to the microwells coated with monoclonal antibody sequentially, and then combining with HRP-labeled vitamin D antibody to form an antibody-antigen-enzyme-labeled antibody complex, which is thoroughly washed and then added with substrate TMB to develop color. TMB is converted to blue under the catalysis of HRP enzyme and to the final yellow under the action of acid. There was a positive correlation between the depth of color and vitamin D in the sample. The absorbance (OD value) was measured with a microplate reader at a wavelength of 450nm, and the concentration of human vitamin D in the sample was calculated by the standard curve. | ||||||||||||||||||||||||
| Source | Human | ||||||||||||||||||||||||
| Synonym | Human VD ELISA Kit | ||||||||||||||||||||||||
| Composition | Kit composition:
|
||||||||||||||||||||||||
| General Notes | 1. The kit should be taken out of the refrigerated environment and balanced at room temperature for 1 hour before it can be used. If the enzyme-labeled coated plate is not used up after opening, the strips should be stored in a sealed bag. 2. Crystals may precipitate in the concentrated washing liquid. When diluting, it can be heated in a water bath to help dissolve, and the results will not be affected during washing. 3. A sampler should be used in each step of sampling, and its accuracy should be checked frequently to avoid test errors. It is best to control the sample addition time within 5 minutes. If the number of specimens is large, it is recommended to use a row gun to add samples. 4. Please make a standard curve at the same time of each measurement, preferably a double hole. If the content of the substance to be tested in the sample is too high (the OD value of the sample is greater than the OD value of the first well of the standard well), please dilute it with the sample diluent for a certain multiple (n times) before measuring, and please finally multiply it by the total dilution multiple (× n × 5) when calculating. 5. The sealing film is only for one-time use to avoid cross-contamination. 6. Please keep the substrate away from light. 7. Strictly follow the instructions, and the test results must be determined based on the reading of the microplate reader. 8. All samples, washing solutions and various wastes should be treated as infectious agents. 9. Components of different batch numbers of this reagent shall not be mixed. |
||||||||||||||||||||||||
| Storage Temp. | Store at 2-8 °C, shelf life 6 months. | ||||||||||||||||||||||||
| Test Range | 50ug/L -1600ug/L | ||||||||||||||||||||||||
| Applications | It is used to determine the content of vitamin D in human serum, plasma and related fluid samples. |
Shipping Notes
- Free Standard Shipping on $100+ Orders to the USA.
- Except Preorder products are shipped in 48 hours.
- Delivery to the USA:
- Standard Shipping : 3-10 business days
- If time is of the essence, please consider selecting expedited delivery for faster service.
Exchange/Return Notes
- We offer a 30-day return/exchange service after receiving.
- Final sale items are not eligible for returns or exchanges.
- To process your return/exchange, please contact us at [email protected]
- Please click here for more details>>> Return & Exchange Policy
4.2 ★★★★★
Based on 26 reviews
Sort
Product Reviews
★★★★★ 5
Mervakon Durable Dog Ball for Medium & Large Dogs - Tough TPU Chew Resistant Interactive Toy
Color: Classic Blue, Color: Classic Blue
This Mervakon Durable Dog Ball for Medium & Large Dogs - Tough TPU Chew Resistant Interactive Toy, Automatic Moving Bouncing Ball for Aggressive Chewers, Classic Blue Indoor Dog Toy is very well-made, easy-to-use and easy-to-clean. This Interactive Dog Ball engages your pet by bouncing automatically. This is a reasonably priced, rechargeable and waterproof tough TPU chew-resistant Dog Ball suitable for both indoor and outdoor play.
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on June 12, 2026
★★★★★ 5
The Toy That Tires Out Your Dog So You Don’t Have To!
Color: Classic Blue, Color: Classic Blue
After trying a different interactive ball previously, I can confidently say this one is built much better. The construction feels more durable, and most importantly, it has held up well against my dog's enthusiastic chewing and chasing. Many interactive toys claim to be tough, but this one has actually survived extended play sessions without being torn apart.
The charging design is simple and well thought out. The ball twists apart easily to reveal the USB-C charging port, making recharging quick and convenient. Once charged, you simply twist the halves back together, press the power button, and it's ready to go. I appreciate that there are no complicated steps or special charging accessories required.
What really stands out is how engaging the toy is. The unpredictable movement keeps my dog interested and encourages plenty of exercise and mental stimulation. Instead of losing interest after a few minutes, my dog continued chasing, pawing, and interacting with the ball until he was genuinely worn out. For pet owners looking for a way to help burn off excess energy, this toy definitely does the job.
One minor issue I noticed is that on a couple of occasions, my dog was able to accidentally turn the toy off while carrying it in his mouth. It wasn't a frequent problem, but it did happen enough to be worth mentioning. Fortunately, turning it back on is quick and easy.
Overall, this has been one of the better interactive dog toys I've tested. It's durable, easy to charge, keeps dogs engaged, and provides the kind of activity that leaves them happily exhausted afterward. If your dog enjoys chasing moving toys and you want something sturdier than many of the alternatives on the market, this is a solid choice.
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on June 3, 2026
★★★★★ 3
Great Entertainment, Short Battery Life
Color: Classic Blue
This is the first interactive ball I've found that my dog hasn't managed to destroy, which is impressive on its own. The ball keeps him entertained for long periods, and he loves chasing, nudging, and following it around the house. The material feels durable and has held up well to regular play. The one button operation is convenient, and the ball is easy to recharge and clean.
My biggest complaint is the battery life. On a full charge, it only seems to run for about 10 minutes before needing to be recharged again. Because of that, I find myself charging it frequently. Even with the shorter runtime, it's been one of the more engaging and durable interactive toys we've tried. Overall, a fun and sturdy toy that keeps dogs occupied, but be prepared for frequent charging.
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on June 5, 2026
★★★★★ 5
Fun Dog Toy
Color: Classic Blue
This toy has been a lot of fun for our dogs. One of my dogs absolutely loves balls and is perfectly content to just carry this around in his mouth. The outer shell feels like a firm foam material. It is durable while still being lightweight enough for the dogs to enjoy.
The ball screws together, but it is designed well enough that you are not constantly worried about it coming apart. You have to intentionally press and twist it to open it, so normal dog play has not caused any issues. Inside is the motorized component, which is easy to turn on and recharge. It offers a few different vibration modes, which keeps things interesting.
The reactions from our dogs have been hilarious. One dog is convinced the ball might be plotting against him and keeps a suspicious distance. Another dog loves it so much that he holds it in his mouth while it vibrates, making his entire head shake. Our third dog plays with it exactly as intended, chasing it around and interacting with it normally.
The motor has enough power to make the ball bounce, roll, and move unpredictably across the floor, which keeps the dogs engaged. It has been a great addition to our toy collection and provides a different kind of enrichment than a standard ball. Overall, the dogs really enjoy it.
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on May 29, 2026
★★★★★ 4
A fun toy
Color: Classic Blue
Our Malamute loves this ball. He likes to carry it around with him and let it will out of his grasp and pick it back up.
The outer ball it tough and durable, and so far it has held up well during play.
It does bounces more than rolls I expected a little more movement across the floor in a more rolling fashion... like those weasel balls from the 90s, but tends to hop and bounce in place. It does works best on hard floor where it has enough contact to move around and keep the dog engaged.
To charge you have to unscrew the ball in half to access the motor unit, and there is grease on the motor, so be careful when opening it and handling the internal piece. It is not difficult, but it is a little more complicated than a simple plug-in charging port.
The button on is also super hard to press. I you have to really squeeze it and even use a tool to turn it on. It has 2 modes though it is so hard to press I am not sure which mode I actually turn on.
It so far has been a fun toy for our young dog and has lasted our extreme chewer (only supervised play)
I would recommend if it went on sale.
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on June 1, 2026