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Description
Mouse Hepatocarcinoma Organoid Culture Medium KitProduct Specification Usage 1. Primary (1) The extracted tissue must be placed in a pre cooled (2 8 C) tissue preservation solution E sampling bottle, quickly transported to a clean laboratory for tissue treatment and cell separation, photographed, and registered with information<(2) Prepare several culture dishes and add 4 pre cooled primary culture buffer B for later use<(3) Disinfect the sampling bottle, place the tissue in a culture dish, clean it
Product Specification
| Usage |
1. Primary (1) The extracted tissue must be placed in a pre cooled (2-8° C) tissue preservation solution E sampling bottle, quickly transported to a clean laboratory for tissue treatment and cell separation, photographed, and registered with information<(2) Prepare several culture dishes and add 4 ℃ pre cooled primary culture buffer B for later use<(3) Disinfect the sampling bottle, place the tissue in a culture dish, clean it three times with primary culture buffer B, and then use an ophthalmic scissors or surgical knife to cut the tissue into tissue blocks with a volume of approximately 1-3mm<(4) The tissue was digested with the digestive fluid C of pig normal lung primary tissue, and shaken at 37 ℃ for 20-25 minutes (observe the digestion process at any time)<(5) Take a small amount of liquid and observe under a microscope. After observing a large number of individual cells or cell clusters below 70um, add three times the volume of primary culture buffer B to terminate digestion<(6) Filter using a 100um pore sieve, collect the filtrate, concentrate and centrifuge at 300g for 5 minutes, remove the supernatant, add primary culture buffer B, and resuspend and centrifuge (7) Matrix adhesive calculation: After step 6, observe the collected tissue volume and add 25 times the tissue volume of matrix adhesive (ABS9495) to resuspend the board 2. Organ like passage culture (1) Pipette the culture medium and add 1-2ml of 4 ℃ organ like passage culture buffer G to each well for 2 minutes<(2) Gently blow the matrix adhesive with a pipette, collect it in a 15ml centrifuge tube, and let it stand at 4 ℃ for 10 minutes. (Each 6-8 wells is a group) (3) a: If the number of organoids is insufficient or the volume is small, centrifuge for 5 minutes to discard the supernatant. Add an appropriate amount of organoid passage culture buffer G and resuspend it into a 1.5ml centrifuge tube. Centrifuge for 5 minutes to discard the liquid and proceed to step 4  & Nbsp& Nbsp& Nbsp& Nbsp; b: When the number of organoids is large or the volume is large: centrifuge for 5 minutes to discard the supernatant, add an appropriate amount of organoid passage digestion solution D for 2-3 minutes, add organoid passage culture buffer G to terminate digestion, centrifuge for 5 minutes to discard the mixture, add an appropriate amount of organoid passage culture buffer G and resuspend into a 1.5ml centrifuge tube, centrifuge for 5 minutes to discard the liquid for step 4 3. Organ like cryopreservation (1) Pipette the culture medium and add 1-2ml of 4 ℃ organ like passage culture buffer G to each well for 2 minutes<(2) Gently blow the matrix adhesive with a pipette, collect it in a 15ml centrifuge tube, and let it stand at 4 ℃ for 10 minutes. (Each 6-8 wells is a group) (3) Centrifuge for 5 minutes to discard the supernatant, add an appropriate amount of organoid passage culture buffer G, and resuspend again. Centrifuge for 5 minutes at 300g to discard the liquid (4) Add an appropriate amount of organoid cryopreservation solution F, gently blow and resuspend, taking a 24 well cell culture plate as an example: the density is 2 wells, and one tube is frozen, with a volume of 1.4ml per tube (5) Mark the information properly, cool down the program, and then transfer it to liquid nitrogen for long-term storage 4. Organ like resuscitation (1) Take 10ml of organ like passage culture buffer G and place it in a 15ml centrifuge tube<(2) Remove frozen organoid cells from a liquid nitrogen tank and quickly melt them in a 37 ℃ water bath(5) Suspension of matrix adhesive, with 25 ul of matrix adhesive per well laid on a 24 well cell culture plate, placed in a culture chamber for 10-15 minutes to form gel, and added with 500 ul of pig normal lung organ culture medium A. |
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| Description |
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| Storage Temp. | 4° C. 3months; -20° C. 1 year, see label for details |
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4.2 ★★★★★
Based on 5 reviews
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Product Reviews
★★★★★ 5
Perfect for aggressive chewers
I have a 2 year old pittie and she chows thru bones in minutes, even those reviewed as "long lasting". This thing is still going strong after 9 months. She was not initially interested, had to spread peanut butter on one end, one time, and now it is her go-to chew toy. Looks a little mangled after all this time but she's not even 75% thru it. Highly recommend, no stomach issues.
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Reviewed in the United States on June 7, 2026
★★★★★ 5
Great, durable bone
My dog loves this bone! It’s super durable and has lasted a few months. Keeps my golden busy! Definitely will buy again.
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Reviewed in the United States on June 9, 2026
★★★★★ 5
Best long-lasting dog chew
If you want something to occupy your dog that's harmless, this is the one. He doesn't have to chase it, so it doesn't get lost under the cabinets, and he's been chewing it for hours and barely made a mark on it. When he's chewing on this, he's not whining or asking to sit on my lap, and he can stay nearby as he enjoys this toy.
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Reviewed in the United States on June 12, 2026
★★★★★ 4
Not perfect for every dog, but still worth a shot
I bought the bacon-flavored Benebone with high hopes after reading the reviews. Our 45lb mutt loves to chew on bones (and thankfully that's ALL she loves to chew on!) but we've had trouble finding anything that she can't destroy in one sitting. We've heard that antlers are the longest lasting but can be damaging to the dog's teeth, so we wanted to try something with a little less potential for disaster.
The Benebone wishbone fared okay. I do have to say that she loved the bone, as did our 14lb schnoodle. The first day she had it, they took turns gnawing on it for hours. Unfortunately, the large one's jaws are quite strong, and so by the end of day 1 there were some pretty significant teething marks on each of the ends, and it was starting to look pretty gnarly. I put it up for a little bit, but they've had other nylon bones that looked worse so I gave it back to them the next day.
On day 2, the little one was chewing on it so vigorously (and I think the bone was gnarly enough) that her gums started bleeding. I noticed the blood on the bone so I took it away again.
On day 3, I gave the bone back to the large one to chew on. It wasn't too long, though, until I was hearing some pretty loud snaps and I found that the large pup was actually starting to get larger pieces off of the ends of the wishbone. This had me worried, so I threw the wishbone away.
Relative to other chew toys that the large one likes (she'll only chew on bones and edibles, for some reason - nothing with any sort of rubbery material), this did actually have a decent life span, but we're still looking for something better.
I give the product 3 stars because the dogs clearly loved the bone, and I'm sure that it's perfect for some dogs. For example, if it was just our schnoodle chewing on it, it would have lasted much longer and I would probably be posting a 5-star review here. In the end, though, we did buy the wishbone for the larger dog, and it failed to hold up for her - for the price, I would have wanted something that lasts longer - hence the two star deduction.
UPDATE 8/10/2014:
Not long after I posted this review, Benebone contacted me in the comments to offer a refund and hinted at the potential for a larger bone sometime in the future. I sent in our order number and they processed the refund quickly. I've added back an extra star, because Benebone's stellar customer service has made the product worth the try. I would definitely do business with Benebone again, and I'm looking forward to trying (or rather, having my pup try) a larger version of the bone if/when it surfaces!
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Reviewed in the United States on August 7, 2014
★★★★★ 5
I Import These to Mexico That's How Good They Are
I have purchased this bone at least 10 times and will keep buying it indefinitely. I have 8 rescue dogs, all extreme chewers , we're talking dogs that destroy absolutely everything. This is the only bone I've found that holds their interest and keeps them coming back for more. I've tried every type of bone on the market. This one wins!
My dogs range from 5 lbs to 100 lbs and every single one of them is equally obsessed. I now order 5 at a time because I know they'll all be used. The price point is excellent for what you get, and after constant use across 8 dogs, I haven't noticed any negative effects on their health or teeth.
I love these so much that I actually import them to Mexico, that should tell you everything. Every time someone visits and sees how occupied and happy my dogs are, they immediately ask where they can get them too.
Benebone, you have a customer for life!!!!!!
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Reviewed in the United States on April 28, 2026