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Description
Gellan GumGellan gum, an anionic polysaccharide, is employed as a replacement for agar in various clinical bacteriological and plant tissue culture media. Its structural composition comprises a repeating tetrasaccharide unit, including two D glucose residues, one L rhamnose residue, and one D glucuronic acid residue. It is an excellent alternative for nutrient media in plant tissue culture, yielding a clearer and more transparent gel than agar. Additionally, it
Gellan gum, an anionic polysaccharide, is employed as a replacement for agar in various clinical bacteriological and plant tissue culture media. Its structural composition comprises a repeating tetrasaccharide unit, including two D-glucose residues, one L-rhamnose residue, and one D-glucuronic acid residue.
It is an excellent alternative for nutrient media in plant tissue culture, yielding a clearer and more transparent gel than agar. Additionally, it requires only half the concentration of agar typically used in tissue culture media.
The Benefits of Gellan Gum
- Easy to use
- Acts as a solid support for the growth and development of explants
- Allows the absorption of nutrients by the plant cells
- The transparency of the gel allows one to clearly see the development of roots or contamination from the start.
- Cost-effective
- Solidifying agents play a crucial role in tissue culture media by providing the necessary support to hold cells, tissues, or explants in place and ensure they receive optimal nutrients for growth and development.
How To Use Gellan Gum
Follow the steps below to prepare 1 liter of tissue culture media using PCT’s Gellan Gum:
- Take a beaker and add 800 ml of distilled water to it.
- To the water add PCT’s MS Media (or alternative media) 4.54 grams, 30 grams of sugar, and PPM™ (generally 1-2 ml/L).
- Make up the volume of the media to 1000ml.
- Adjust the pH of the media between 5.4-5.8.
- Add 3-4 g/L of PCT’s Gellan Gum to the media and keep stirring.
- Autoclave the medium for 20 minutes at 15 psi and 121℃.
Your media is ready!
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4.3 ★★★★★
Based on 5 reviews
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Product Reviews
★★★★★ 3
Madison Deserved Better
Format: Kindle
Madison was a beta...except she wasn't any longer. She was a late presenting Omega. And she was struggling. She was tall and thin, not tiny and curvy. She was opinionated. She was everything an Omega was not. After suffering through her first heat, her friends took her to Ardor, a club where Omegas came to safely find Alphas. She's not expecting much but then she connects with a sexy beta. And when she meets his Alphas, they set her body on fire. Maybe, she's found her no-strings-attached heat pack. Maybe, she's found something more.
I could not connect with the characters in this book, so their story never resonated with me. And there was no love story; there was sex. Grey made it clear from the beginning that he had a true love and it was his beta boy, Rian. He went so far as to reassure Rian “Say the word, I’ll never touch her again. Lucas can put the babies in her. I only need you, beta boy”. So, Madison was there for babies, no emotions needed. Nice. No, thank you. I want the Omega to be the center of their world, not an incubator. Lucas and Rian weren't any better. After her heat, they let her leave. Not one of them made her feel valued. No one gave her a reason to stay or even offered a cuddle. And the sex didn't even come across as mind-blowing. Madison deserved better.
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Reviewed in the United States on March 11, 2025
★★★★★ 5
No breakup, very sweet, instalove
Format: Kindle
Omegaverse and doesn't disappoint! Sweet guys, newly Omega FMC. The boyfriends are boyfriends. What's not to love? No angst, no breakup.
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Reviewed in the United States on February 23, 2025
★★★★★ 4
Pretty Darn Good
Format: Kindle
So I’ve been on a omega kick and this definitely hit the spot. Madison was frustrating at times with how she acted towards Lucas, Gray, and Rian. It was like she said towards the end, she didn’t believe she deserved nice things.
It would have been nice to hear from her best friends again. They kind of were there in the beginning and the gone except for mention of text messages received from them. I feel like her friends would have been great help in encouraging Madison to go with the pack and never give Brent another chance because he was toxic.
I loved Rian. His personality was awesome. His humor. His ability to make Madison comfortable whenever she was feeling overwhelmed. And the fact he fell for her and she fell for him first. They are cute together.
I do feel like Lucas was the odd man out though. Like Lucas didn’t develop as much of a relationship with Madison. I would have really liked to see more development in the relationship between them. It was also the same with him and Rian. There is really no relationship displayed. Most of the relationship being displayed is between Rian and Gray.
Nevertheless, I loved reading about the dynamic that came to fruition during the entirety of this story. Madison finally got her happiness. And Brent finally got punched in the face. Everyone got exactly what they deserve.
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Reviewed in the United States on September 6, 2022
★★★★★ 3
Pack's Promise was okay but not great
Format: Kindle
Pack's Promise was okay but not great. I won't recommend it to anyone that I know.
PRO:
* Very likable characters
* Lots of steamy scenes that are written very well
* The spelling and grammar are good
* The punctuation is good with the exception of using hyphens instead of commas. Lots of hyphens. Lots and lots of hyphens.
CON:
* Almost no interactions with any characters outside of Madison and the pack
* Nearly no plot. They meet, get together for a heat, agree to make it permanent, done
* Quite a few typos such as extraneous words, missing words and words out of order
THINGS TO KNOW:
* More steamy scenes than storytelling
* A lot of MM & MMM, some MFMM during heat
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Reviewed in the United States on January 5, 2023
★★★★★ 5
such a good read
Format: Kindle
Madison, Lucas, Grey and Rian were made for each other!!! First time reading from this author and I’m not disappointed!!! Absolutely love the Love in this book and couldn’t ask for a better OV!
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Reviewed in the United States on October 25, 2023