SKU: 32079881893

Mouse Pro-C3 ELISA Kit

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Description

Mouse Pro-C3 ELISA KitProduct Specification Usage Specimen Requirements 1. Specimens should be extracted as soon as possible after collection, following the relevant literature. Experiments should be performed as soon as possible after extraction. If experiments cannot be performed immediately, specimens can be stored at 20C, but repeated freeze thaw cycles should be avoided. 2. Samples containing sodium nitrate (NaN3) cannot be assayed, as NaN3 inhibits horseradish

Product Specification


3. Incubation: Seal the plate with a sealing film and incubate at 37°C for 30 minutes.

4. Prepare the solution: Dilute the 30x concentrated wash solution 30x with distilled water and set aside.

5. Wash: Carefully remove the sealing film, discard the liquid, spin dry, fill each well with wash solution, let it sit for 30 seconds, then discard. Repeat this process 5 times and pat dry.

6. Add enzyme: Add 50 μl of enzyme-labeled reagent to each well, excluding the blank well.

7. Incubation: Perform the same procedure as in step 3.

8. Wash: Perform the same procedure as in step 5.

9. Color Development: Add 50 μl of Color Developing Reagent A to each well, followed by 50 μl of Color Developing Reagent B. Gently shake to mix thoroughly. Incubate at 37°C in the dark for 10 minutes.

10. Stop: Add 50 μl of Stop Solution to each well to terminate the reaction (the blue color will immediately turn yellow).

11. Measurement: Use the blank well as a zero value and measure the absorbance (OD) of each well at 450 nm. Measurements should be performed within 15 minutes after adding the Stop Solution.

Calculation


Draw a standard curve on graph paper with the concentration of the standard as the horizontal axis and the OD value as the vertical axis. Find the corresponding concentration of the sample from the standard curve based on the OD value of the sample; then multiply it by the dilution factor. Alternatively, use the concentration of the standard and the OD value to calculate the linear regression equation of the standard curve, substitute the OD value of the sample into the equation to calculate the sample concentration, and then multiply it by the dilution factor to obtain the actual concentration of the sample.

Standard Curve

Usage

Specimen Requirements


1. Specimens should be extracted as soon as possible after collection, following the relevant literature. Experiments should be performed as soon as possible after extraction. If experiments cannot be performed immediately, specimens can be stored at -20°C, but repeated freeze-thaw cycles should be avoided.

2. Samples containing sodium nitrate (NaN3) cannot be assayed, as NaN3 inhibits horseradish peroxidase (HRP) activity.

Procedure


1. Dilution of Standard: This kit provides one standard sample at full strength. Users can dilute the sample in a small test tube according to the following chart. 10μg/L

Standard No. 5

Add 150μl of the original standard to 150μl of the standard diluent

5μg/L

Standard No. 4

Add 150μl of the original standard to 150μl of the standard diluent

Standard No. 3

Add 150μl of Standard No. 4 to 150μl of Standard Diluent

1.25μg/L

Standard No. 2

Add 150μl of Standard No. 3 to 150μl of Standard Diluent
Species Reactivity Mouse
Theory This kit uses a double-antibody sandwich assay to measure mouse type III collagen propeptide (Pro-C3) levels in samples. A microplate is coated with purified mouse type III collagen propeptide (Pro-C3) antibody to create a solid-phase antibody. Type III collagen propeptide (Pro-C3) is then added sequentially to the antibody-coated microwells. This antibody then binds to HRP-labeled type III collagen propeptide (Pro-C3) antibody, forming an antibody-antigen-enzyme-labeled antibody complex. After thorough washing, the assay is developed with the substrate TMB. TMB converts to blue under the catalysis of HRP and to yellow under the action of acid. The intensity of the color is positively correlated with the amount of type III collagen propeptide (Pro-C3) in the sample. The absorbance (OD) is measured at 450 nm using a microplate reader, and the concentration of mouse type III collagen propeptide (Pro-C3) in the sample is calculated using a standard curve.
Detection Type Used to determine the content of type III collagen propeptide (Pro-C3) in mouse serum, plasma and related liquid samples.
Composition

Serial number

Component name

Specification

Serial number

Component name

Specification

1

30 times concentrated detergent

20ml×1 bottle

6

Developer B solution

6ml×1/bottle

2

Enzyme Labeled Reagent

6ml×1 bottle

7

Stop Solution

6ml×1 bottle

3

Enzyme-coated plate

12 wells ×8 strips

8

Standard (20 μg/L)

0.5 ml ×1 bottle

4

Sample diluent

6 ml ×1 bottle

9

Standard diluent

1.5ml×1 bottle

5

Developer A Liquid

6ml×1 bottle

10

Sealing film

2 photos
General Notes 1. After removing the kit from the refrigerator, allow it to equilibrate at room temperature for 1 hour before use. If the enzyme-coated plate is opened and not completely used, store it in a sealed bag. 2. Crystals may form in the concentrated wash buffer. Warming in a water bath can aid dissolution during dilution. This will not affect the results. 3. Use a pipette for each sample addition step and frequently calibrate its accuracy to avoid experimental error. The time for each addition should ideally be within 5 minutes. For large numbers of samples, using a dispenser is recommended. 4. Develop a standard curve with each measurement, preferably with replicates. If the analyte content in the sample is too high (the sample OD value is greater than the OD value of the first standard well), dilute the sample a certain number of times (n times) with sample diluent before measurement. When calculating the final dilution, multiply by the total dilution factor (×n×5).

5. The sealing film is for single use only to avoid cross contamination.

6. Please store the substrate in a dark place.

7. Strictly follow the instructions for operation. The test results must be determined based on the readings of the microplate reader.

8. All samples, washing solutions and various wastes should be treated as infectious materials.

9. Components of different batches of this reagent must not be mixed.
Storage Temp. Unopened test kit, stored at 2-8°C, has a shelf life of 6 months.
Test Range 0.3μg/L-12μg/L
Shipping Notes
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Exchange/Return Notes
  • We offer a 30-day return/exchange service after receiving.
  • Final sale items are not eligible for returns or exchanges.
  • To process your return/exchange, please contact us at [email protected]
  • Please click here for more details>>> Return & Exchange Policy
SKU: 32079881893

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Cristy L. Spencer
Port Orchard, US
★★★★★ 5
Perfect for my mid size pit bull
Color: Ultra, Size: XL
Updating my review, the ball only lasted 5 days. I left the 5 stars because we had enough fun those 5 days to be worth the small price. She has dog has a lot of jaw strength, so I’m not surprised We have a mid size foster pit bull. This ball is her favorite toy. It’s durable and the perfect size for her to play ball safely.
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Reviewed in the United States on February 22, 2026
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cassidy y
Belleville, US
★★★★★ 5
Good quality
Color: Ultra, Size: Large, Color: Ultra, Size: Large
Great quality. I purchased for my daughter’s dog who destroys all toys. This toy holds up. I didn’t realize it was a large and the chickit stick she had was a medium. The large ball ended up working out good for playing inside the house. The dog likes it a lot.
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Reviewed in the United States on May 3, 2026
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Mindhealer
Pawtucket, US
★★★★★ 4
Please read!
Color: Ultra, Size: Large
This dog ball is so useful! We have a large property and we use the Chuckit! thrower with the ball. The color and material of the toy is very high-quality and lasts for a long time. We have been using the Chuckit! brand for a long time now and we love their dog products! It is a good price and we buy replacement balls like once or twice every 6 months because they keep getting lost in bushes or trees 😂 After some time the color of the ball gets washed out and since our dogs are aggressive chewers, one of the balls we got broke and split open, but it was still usable! The reason that one broke is because we had it for a HECKA LONG TIME! It has no squeaker so when your dog has the ball it wont make any loud and annoying sounds. It is easy to pick up with the thrower and it seems durable and sturdy enough to last them a few months. I don't recommend as a cat toy because this ball is specifically designed for dogs. But is your cat likes fetch i guess it could work! The ball is soft enough to not be hard as steel, but is sturdy enough to not break immediately.
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Reviewed in the United States on August 9, 2025
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Hayleysgirl
Charlottesville, US
★★★★★ 5
Chuck-it stands up to huskies teeth.
Color: Ultra, Size: Large
This item is awesome. Stands up to my huskies teeth.
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Reviewed in the United States on May 14, 2026
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D.G.
Omaha, US
★★★★★ 5
Great ball!
Color: Ultra, Size: Large
Our puppy loves this ball. It has held up well so far, it’s about 2 weeks now of large breed pup play. It’s been outside for the last week, rain or shine, still in working order. Not as big as I thought it would be though. No squeak, bounces well. Thick rubber like material, that can be depressed when biting down.
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Reviewed in the United States on May 14, 2026

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