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Description
Rat IL-17 ELISA KitProduct Specification Usage Experimental equipment required for the experiment: 1. Microplate reader (450nm) 2. High precision pipette and gun tips: 0. 5 10uL, 5 50uL, 20 200uL, 200 1000uL 3. 37 constant temperature box 4. Distilled water or deionized water Sample processing and requirements: Serum: Place the whole blood sample collected in the serum separation tube at room temperature for 2 hours or at 4 overnight, then centrifuge at 1000g for 20
Product Specification
| Usage |
Experimental equipment required for the experiment: 1. Microplate reader (450nm) 2. High-precision pipette and gun tips: 0.5-10uL, 5-50uL, 20-200uL, 200-1000uL 3. 37℃ constant temperature box 4. Distilled water or deionized water Sample processing and requirements: Serum: Place the whole blood sample collected in the serum separation tube at room temperature for 2 hours or at 4℃ overnight, then centrifuge at 1000×g for 20 minutes, and take the supernatant, or store the supernatant at -20℃ or -80℃, but avoid repeated freezing and thawing. Plasma: Collect the specimen using EDTA or heparin as an anticoagulant. Centrifuge the specimen at 1000 × g for 15 minutes at 2-8°C within 30 minutes of collection. The supernatant can be assayed or stored at -20°C or -80°C, but avoid repeated freezing and thawing. Tissue homogenization: Rinse the tissue with pre-chilled PBS (0.01M, pH 7.4) to remove residual blood (lysed red blood cells in the homogenate will affect the measurement results). Weigh the tissue and mince it. Add the minced tissue to the appropriate volume of PBS (generally a 1:9 weight-to-volume ratio, e.g., 1 g of tissue sample to 9 mL of PBS. The specific volume can be adjusted according to experimental needs and recorded. It is recommended to add protease inhibitors to the PBS) in a glass homogenizer and grind thoroughly on ice. To further lyse tissue cells, the homogenate can be sonicated or repeatedly frozen and thawed. Finally, centrifuge the homogenate at 5000 × g for 5-10 minutes, and the supernatant can be assayed. Cell Lysis Buffer: Gently wash adherent cells with ice-cold PBS, then trypsinize and collect cells by centrifugation at 1000×g for 5 minutes. Suspension cells can be collected directly by centrifugation. Wash collected cells three times with ice-cold PBS and resuspend in 150-200 μL of PBS per 1×10^6 cells (it is recommended to add protease inhibitors to the PBS; if the cell count is very low, reduce the PBS volume appropriately). Disrupt the cells by repeated freeze-thaw cycles or sonication. Centrifuge the extract at 1500×g for 10 minutes at 2-8°C, and remove the supernatant for analysis. Cell Culture Supernatant: Centrifuge at 1000×g for 20 minutes. Remove the supernatant for analysis or store at -20°C or -80°C, avoiding repeated freeze-thaw cycles. Other biological fluids: Centrifuge at 1000xg for 20 minutes, remove the supernatant, and test. Pre-test preparation: 1. Remove the test kit from the refrigerator 10 minutes in advance and equilibrate to room temperature. 2. Prepare the standard gradient working solution: Add 1mL of universal diluent to the lyophilized standard, let it stand for 15 minutes to completely dissolve, then gently mix (concentration is 300pg/mL). Then dilute to the following concentrations: 300pg/mL, 150pg/mL, 75pg/mL, 37.5pg/mL, 18.75pg/mL, 9.375pg/mL, 4.6875pg/mL, and 0pg/mL. Serial dilution method: Take seven EP tubes and add 500uL of universal diluent to each. Pipette 500uL of the 300pg/mL standard working solution into the first EP tube and mix thoroughly to make a 150pg/mL standard working solution. Repeat this procedure for subsequent tubes. The last tube serves as a blank well; there is no need to pipette liquid from the penultimate tube. See the figure below for details. 3. Preparation of biotinylated detection antibody working solution: Centrifuge the concentrated biotinylated antibody at 1000×g for 1 minute 15 minutes before use. Dilute the 100× concentrated biotinylated antibody to a 1× working concentration with universal diluent (e.g., 10uL concentrate + 990uL universal diluent). Prepare and use immediately. 4. Prepare the enzyme conjugate working solution: 15 minutes before use, centrifuge the 100× concentrated enzyme conjugate at 1000×g for 1 minute. Dilute the 100× concentrated HRP enzyme conjugate to a 1× working concentration with universal diluent (e.g., 10 μL of concentrate + 990 μL of universal diluent). Prepare immediately. 5. Prepare the 1× wash solution: Dispense 10 mL of 20× wash solution into 190 mL of distilled water (concentrated wash solution removed from the refrigerator may crystallize; this is normal. Allow to stand at room temperature until the crystals have completely dissolved before preparing). Procedure: 1. Remove the desired strips from the aluminum foil bag after equilibration at room temperature for 10 minutes. Seal the remaining strips in a ziplock bag and return to 4°C. 2. Sample addition: Add 100 μL of sample or standard of varying concentrations to the corresponding wells. Add 100 μL of universal diluent to the blank wells. Cover with a film and incubate at 37°C for 60 minutes. (Recommendation: Dilute the sample to be tested at least 1-fold with universal diluent before adding it to the ELISA plate. This will reduce the impact of matrix effects on the test results. The sample concentration should be multiplied by the corresponding dilution factor when calculating the final sample concentration. It is recommended to run replicates for all test samples and standards.) 3. Add Biotinylated Antibody: Remove the ELISA plate and discard the liquid without washing. Add 100 μL of Biotinylated Antibody Working Solution directly to each well. Cover with a film and incubate at 37°C for 60 minutes. 4. Wash: Discard the liquid and add 300 μL of 1x Wash Solution to each well. Let stand for 1 minute, shake off the wash solution, and pat dry on absorbent paper. Repeat this process three times (a plate washer can also be used). 5. Add Enzyme Conjugate Working Solution: Add 100 μL of Enzyme Conjugate Working Solution to each well. Cover with a film and incubate at 37°C for 30 minutes. 6. Washing: Discard the liquid and wash the plate five times as in step 4. 7. Adding substrate: Add 90 μL of substrate (TMB) to each well, cover with a sealing film, and incubate at 37°C in the dark for 15 minutes. 8. Adding stop solution: Remove the ELISA plate and add 50 μL of stop solution directly to each well. Immediately measure the OD value of each well at a wavelength of 450 nm. Calculating experimental results: 1. Calculate the average OD value of the standard and sample replicates and subtract the OD value of the blank well as a correction factor. Plot the standard curve of the four-parameter logistic function on double-logarithmic graph paper, with concentration as the horizontal axis and OD value as the vertical axis. 2. If the sample OD value is higher than the upper limit of the standard curve, dilute the sample appropriately and retest. Multiply the sample concentration by the corresponding dilution factor. |
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| Theory | This kit utilizes a double-antibody sandwich enzyme-linked immunosorbent assay (ELISA). Sample, standard, biotin-labeled detection antibody, and HRP conjugate are sequentially added to microwells pre-coated with Interleukin 17 (IL-17) capture antibody. After incubation and washing, the sample is developed using the substrate TMB. TMB converts to blue under the catalysis of HRP and to yellow under the action of acid. The intensity of the color is positively correlated with the amount of Interleukin 17 (IL-17) in the sample. The absorbance (OD) is measured at 450 nm using a microplate reader to calculate the sample concentration. | |||||||||||||||||||||||||||||||||
| Source | Rat | |||||||||||||||||||||||||||||||||
| Synonym | Rat Interleukin 17 ELISA Kit | |||||||||||||||||||||||||||||||||
| Detection Type | Double antibody sandwich method | |||||||||||||||||||||||||||||||||
| Composition |
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| Background | Interleukin-17 (IL-17), also known as CTLA-8, is a polymorphic cytokine expressed by T cells that shares high homology with the protein encoded by the ORF13 gene of the herpes simplex virus Saimiri. cDNA clones encoding IL-17 have been isolated from activated rat, mouse, and human T cells. The human IL-17 cDNA encodes a 155-amino acid (aa) precursor protein with a 19-aa signal peptide, which is cleaved to produce a 136-aa mature IL-17 containing a potential N-linked glycosylation site. Both recombinant and native IL-17 have been shown to exist as disulfide-linked homogenates. | |||||||||||||||||||||||||||||||||
| General Notes | 1. Strictly adhere to the specified incubation time and temperature to ensure accurate results. All reagents must be at room temperature (20-25°C) before use. Refrigerate reagents immediately after use. 2. Improper plate washing may result in inaccurate results. Ensure that all liquid in the wells is aspirated thoroughly before adding substrate. Do not allow the wells to dry out during incubation. 3. Remove any residual liquid and fingerprints from the bottom of the plate, as this will affect the OD value. 4. The substrate developer solution should be colorless or very light in color. Do not use substrate solution that has turned blue. 5. Avoid cross-contamination of reagents and specimens to prevent erroneous results. 6. Avoid direct exposure to strong light during storage and incubation. 7. Do not expose any reagents to bleaching solvents or the strong fumes emitted by bleaching solvents. Any bleaching agent will destroy the biological activity of the reagents in the kit. 8. Do not use expired products, and do not mix components with different product numbers and batches. 9. Recombinant proteins from sources other than the kit may not be compatible with the antibodies in this kit and will not be recognized. 10. If there is a possibility of disease transmission, all samples should be managed properly and samples and testing devices should be handled according to prescribed procedures. |
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| Storage Temp. | If the unopened kit is stored at 4°C, the shelf life is 6 months. | |||||||||||||||||||||||||||||||||
| Test Range | 4.68-300 pg/mL | |||||||||||||||||||||||||||||||||
| Applications | Serum, plasma, tissue homogenate, cell lysate, cell culture supernatant and other biological fluids |
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4.2 ★★★★★
Based on 25 reviews
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Product Reviews
★★★★★ 5
Super cute
Style: Chain(Gold), Style: Chain(Gold)
Went perfect with all my bikinis in Vegas. Adds the perfect pop to any swimwear.
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on May 10, 2026
★★★★★ 5
Pretty in pink.
Color: Pink
Sticks well, stayed on 2 days until I peeled it off. Largest 3cm star shape & 2.5cm heart shape. Solid pieces are 6-2mm. Very pretty light and bright pink colors.
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Reviewed in the United States on February 23, 2026
★★★★★ 4
Fun, cute, good value
Color: Pink
I got these for the toddler, who is living her best life as a princess. She is allowed to wear these for dress up and she absolutely adores them. I apply them to the apples of her cheeks, on the tip of her nose, and on her arms and ankles like fancy bracelets. At this rate, they won't last long, and because they are used for play, I end up pulling them off the rugs and upholstery for days afterward. It's like a game for mom!
They are lovely and fun and she absolutely adores them. The only downstar is for the adhesive quality. Now, this might be just me because I received them during a severe winter storm and they stayed in the mailbox for several days of below zero weather, but the sticky backing peels off quite easily from many of them. It's easy enough to fix with the tweezers that are supplied, but you might want to pay attention to that detail. At bath time, I am finding many pieces of the tape no longer attached to a jewel. I don't know if my bunch were damaged because of the cold, but if you're planning to go to the club, I would bring extras. If you're using them for playtime, just beware that you might find them in all kinds of unexpected places. They definitely won't hold up to bath time, so take them off first, to avoid clogging up the plumbing.
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Reviewed in the United States on January 27, 2026
★★★★★ 5
Emerald Glow: The Ultimate Festival Essential
Color: Green, Color: Green
I recently picked up these Green Face Gems for a festival, and I have to say, they are an absolute standout for anyone looking to level up their makeup game. The variety of shades in this pack is stunning; you get everything from a vibrant, electric lime to a deep, moody forest green that catches the light beautifully. Unlike some stickers that look flat or plastic, these have a high-quality multifaceted cut that mimics real emeralds, giving off a brilliant sparkle whether you’re under the sun or shifting strobe lights at a rave.
The self-adhesive backing is surprisingly reliable. I was worried about them sliding off halfway through a humid outdoor concert, but they stayed firmly in place through hours of movement. Despite the strong grip, they didn't pull at my skin or leave a sticky residue when it was time to take them off. I also experimented with placing them in my hair—which looked incredible—and they held onto the strands much better than I anticipated. If you’re planning a themed look or just want to add a sophisticated pop of color to your eyes, these gems are a total win. They are easy to apply, comfortable to wear, and genuinely look like professional-grade body art.
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Reviewed in the United States on February 8, 2026
★★★★★ 3
Beautiful gems! However…
Color: Gold
Had to dock 2 stars because the tweezers are too flimsy to actually assist with applying the gems, which sucks because that was a huge selling point for me. Additionally, the glue sticks a little too well to the paper. Many gems detached from the adhesive, which made them useless if the objective is to apply the gems to your face.
Still had to award a few stars because the ones that did manage to successfully pull from the paper without coming apart, stayed on my face for about 3-4 hours outside in the sweaty heat. I anticipated to lose a few but they survived and that impressed me!
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Reviewed in the United States on April 30, 2026