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Description
Human IL1R1 ELISA KitProduct Specification Usage Experimental equipment required for the experiment: 1. Microplate reader (450nm) 2. High precision pipette and gun tips: 0. 5 10uL, 5 50uL, 20 200uL, 200 1000uL 3. 37 constant temperature box 4. Distilled water or deionized water Sample processing and requirements: Serum: Place the whole blood sample collected in the serum separation tube at room temperature for 2 hours or at 4 overnight, then centrifuge at 1000g for 20
Product Specification
| Usage |
Experimental equipment required for the experiment: 1. Microplate reader (450nm) 2. High-precision pipette and gun tips: 0.5-10uL, 5-50uL, 20-200uL, 200-1000uL 3. 37℃ constant temperature box 4. Distilled water or deionized water Sample processing and requirements: Serum: Place the whole blood sample collected in the serum separation tube at room temperature for 2 hours or at 4℃ overnight, then centrifuge at 1000×g for 20 minutes, and take the supernatant, or store the supernatant at -20℃ or -80℃, but avoid repeated freezing and thawing. Plasma: Collect the specimen using EDTA or heparin as an anticoagulant. Centrifuge the specimen at 1000 × g for 15 minutes at 2-8°C within 30 minutes of collection. The supernatant can be assayed or stored at -20°C or -80°C, but avoid repeated freezing and thawing. Tissue homogenization: Rinse the tissue with pre-chilled PBS (0.01M, pH 7.4) to remove residual blood (lysed red blood cells in the homogenate will affect the measurement results). Weigh the tissue and mince it. Add the minced tissue to the appropriate volume of PBS (generally a 1:9 weight-to-volume ratio, e.g., 1 g of tissue sample to 9 mL of PBS. The specific volume can be adjusted according to experimental needs and recorded. It is recommended to add protease inhibitors to the PBS) in a glass homogenizer and grind thoroughly on ice. To further lyse tissue cells, the homogenate can be sonicated or repeatedly frozen and thawed. Finally, centrifuge the homogenate at 5000 × g for 5-10 minutes, and the supernatant can be assayed. Other biological fluids: Centrifuge at 1000xg for 20 minutes, remove the supernatant, and test. Pre-test preparation: 1. Remove the test kit from the refrigerator 10 minutes in advance and equilibrate to room temperature. 2. Prepare the standard gradient working solution: Add 1 mL of universal diluent to the lyophilized standard, let it stand for 15 minutes to completely dissolve, then gently mix (concentration is 10 ng/mL). Then dilute to the following concentrations: 10 ng/mL, 5 ng/mL, 2.5 ng/mL, 1.25 ng/mL, 0.625 ng/mL, 0.3125 ng/mL, 0.15625 ng/mL, and 0 ng/mL. Serial dilution method: Take seven EP tubes and add 500uL of universal diluent to each. Pipette 500uL of the 10ng/mL standard working solution into the first EP tube and mix thoroughly to make a 5ng/mL standard working solution. Repeat this procedure for subsequent tubes. The last tube serves as a blank well; there is no need to pipette liquid from the penultimate tube. See the figure below for details. 3. Preparation of biotinylated detection antibody working solution: Centrifuge the concentrated biotinylated antibody at 1000×g for 1 minute 15 minutes before use. Dilute the 100× concentrated biotinylated antibody to a 1× working concentration with universal diluent (e.g., 10uL concentrate + 990uL universal diluent). Prepare and use immediately. 4. Prepare the enzyme conjugate working solution: 15 minutes before use, centrifuge the 100× concentrated enzyme conjugate at 1000×g for 1 minute. Dilute the 100× concentrated HRP enzyme conjugate to a 1× working concentration with universal diluent (e.g., 10 μL of concentrate + 990 μL of universal diluent). Prepare immediately. 5. Prepare the 1× wash solution: Dispense 10 mL of 20× wash solution into 190 mL of distilled water (concentrated wash solution removed from the refrigerator may crystallize; this is normal. Allow to stand at room temperature until the crystals have completely dissolved before preparing). Procedure: 1. Remove the desired strips from the aluminum foil bag after equilibration at room temperature for 10 minutes. Seal the remaining strips in a ziplock bag and return to 4°C. 2. Sample addition: Add 100 μL of sample or standard of varying concentrations to the corresponding wells. Add 100 μL of universal diluent to the blank wells. Cover with a film and incubate at 37°C for 60 minutes. (Recommendation: Dilute the sample to be tested at least 1-fold with universal diluent before adding it to the ELISA plate. This will reduce the impact of matrix effects on the test results. The sample concentration should be multiplied by the corresponding dilution factor when calculating the final sample concentration. It is recommended to run replicates for all test samples and standards.) 3. Add Biotinylated Antibody: Remove the ELISA plate and discard the liquid without washing. Add 100 μL of Biotinylated Antibody Working Solution directly to each well. Cover with a film and incubate at 37°C for 60 minutes. 4. Wash: Discard the liquid and add 300 μL of 1x Wash Solution to each well. Let stand for 1 minute, shake off the wash solution, and pat dry on absorbent paper. Repeat this process three times (a plate washer can also be used). 5. Add Enzyme Conjugate Working Solution: Add 100 μL of Enzyme Conjugate Working Solution to each well. Cover with a film and incubate at 37°C for 30 minutes. 6. Washing: Discard the liquid and wash the plate five times as in step 4. 7. Adding substrate: Add 90 μL of substrate (TMB) to each well, cover with a sealing film, and incubate at 37°C in the dark for 15 minutes. 8. Adding stop solution: Remove the ELISA plate and add 50 μL of stop solution directly to each well. Immediately measure the OD value of each well at a wavelength of 450 nm. Calculating experimental results: 1. Calculate the average OD value of the standard and sample replicates and subtract the OD value of the blank well as a correction factor. Plot the standard curve of the four-parameter logistic function on double-logarithmic graph paper, with concentration as the horizontal axis and OD value as the vertical axis. 2. If the sample OD value is higher than the upper limit of the standard curve, dilute the sample appropriately and retest. Multiply the sample concentration by the corresponding dilution factor. |
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| Theory | This kit uses a double-antibody sandwich enzyme-linked immunosorbent assay (ELISA). Sample, standard, biotin-labeled detection antibody, and HRP conjugate are sequentially added to microwells pre-coated with Interleukin 1 Receptor Type I (IL1R1) capture antibody. After incubation and washing, the sample is developed using the substrate TMB. TMB is converted to blue by peroxidase (HRP) catalysis and to yellow by acid. The intensity of the color is positively correlated with the amount of Interleukin 1 Receptor Type I (IL1R1) in the sample. The absorbance (OD) is measured at 450 nm using a microplate reader to calculate the sample concentration. | |||||||||||||||||||||||||||||||||
| Source | Human | |||||||||||||||||||||||||||||||||
| Synonym | Human Interleukin 1 Receptor Type I ELISA Kit | |||||||||||||||||||||||||||||||||
| Detection Type | Double antibody sandwich method | |||||||||||||||||||||||||||||||||
| Composition |
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| Background | Interleukin-1 receptor, type I (IL-1R1), also known as CD121a (Cluster of Differentiation 121a), is an interleukin receptor. The protein encoded by this gene is a cytokine receptor belonging to the interleukin-1 receptor family. This protein is a receptor for interleukin-1α (IL1A), interleukin-1β (IL1B), and interleukin-1 receptor antagonist (IL1RA). It is an important mediator involved in many cytokine-induced immune and inflammatory responses. This gene, along with interleukin-1 receptor type II (IL1R2), interleukin-1 receptor class 2 (IL1RL2), and interleukin-1 receptor class 1 (IL1RL1), constitutes a cytokine receptor gene cluster mapped to chromosome 2q12. | |||||||||||||||||||||||||||||||||
| General Notes | 1. Strictly adhere to the specified incubation time and temperature to ensure accurate results. All reagents must be at room temperature (20-25°C) before use. Refrigerate reagents immediately after use. 2. Improper plate washing may result in inaccurate results. Ensure that all liquid in the wells is aspirated thoroughly before adding substrate. Do not allow the wells to dry out during incubation. 3. Remove any residual liquid and fingerprints from the bottom of the plate, as this will affect the OD value. 4. The substrate developer solution should be colorless or very light in color. Do not use substrate solution that has turned blue. 5. Avoid cross-contamination of reagents and specimens to prevent erroneous results. 6. Avoid direct exposure to strong light during storage and incubation. 7. Do not expose any reagents to bleaching solvents or the strong fumes emitted by bleaching solvents. Any bleaching agent will destroy the biological activity of the reagents in the kit. 8. Do not use expired products, and do not mix components with different product numbers and batches. 9. Recombinant proteins from sources other than the kit may not be compatible with the antibodies in this kit and will not be recognized. 10. If there is a possibility of disease transmission, all samples should be managed properly and samples and testing devices should be handled according to prescribed procedures. |
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| Storage Temp. | If the unopened kit is stored at 4°C, the shelf life is 6 months. | |||||||||||||||||||||||||||||||||
| Test Range | 0.15-10 ng/mL | |||||||||||||||||||||||||||||||||
| Applications | Serum, plasma, tissue homogenates and other biological fluids |
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4.5 ★★★★★
Based on 12 reviews
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Product Reviews
★★★★★ 5
The Only SPF My Skin Will Tolerate!
Style: 4 Fl Oz (Pack of 1)
After 20 rounds of radiation, I was given a list of exactly four things I could put on my face--things like water and Vaseline. Of course, I was also instructed to wear SPF in "all visible light." However, finding a sunscreen my skin could tolerate proved to be a challenge, as even those made with clean ingredients, marketed for babies, and/ or claiming to be hypoallergenic resulted in hot, angry rashes. Price point also didn't matter; I tried everything from drugstore to premium brands.
Thankfully, when this product came on the market, my dermatologist suggested I try it. I've been wearing it ever since. It IS thick so I usually rub any remaining face moisturizer into my palms and then add the Sunscreen, rubbing and warming it between my palms to make it more easily spreadable. Then I press it onto the skin on my face with my palms and fingers--I don't like to pull or tug too much at the skin on my face. At first, there is a visible white cast, but it disappears within a couple of minutes.
If I'm really in a hurry, now I can mix a tiny bit of my foundation into the sunscreen so that it spreads faster and I can move on from the white cast faster--I know I lose some sun protection factor that way, but my derm says I also gain some protection in the tinting, so it's okay. The important thing is that I get it on in whatever time I have.
I'm just grateful to have found something that doesn't upset my crazy-sensitive skin! Wishing all you sensitive beauties protected fun in the sun...
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Reviewed in the United States on March 18, 2026
★★★★★ 4
Good sun protection without chemicals, without a high price.
Style: 4 Fl Oz (Pack of 1)
I've bought this a couple of times. I feel like it gives excellent sun protection at a reasonable price. I mostly use it on my face. When I first apply it, it looks a little white and pasty, but it doesn't take long for that to go away. It's a little greasy at first , but I have dry skin, so I it absorbs in. I like using a sunscreen that doesn't have a lot of chemicals. This works well while using ingredients I feel good about putting on my skin.
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Reviewed in the United States on July 13, 2025
★★★★★ 5
A very effective sunscreen at a very reasonable price
Style: 4 Fl Oz (Pack of 1)
This sunscreen is very reasonably priced but would I love about this product more than anything is how effective it is at protecting your skin and will be my first go to when purchasing a sunscreen, and for a man, it is best to be clean shaven when using, and not to put on too much as it will make you look like Casper the ghost if you do
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Reviewed in the United States on May 30, 2026
★★★★★ 5
No scent and blends in surprisingly well.
Style: 4 Fl Oz (Pack of 1)
Eucerin 50 (Sensitive Mineral - Zinc Oxide)
Expiration date: Sept. 2026
Purchase date: May 2025
The most noticeable ingredients in the sunscreen are zinc oxide, mineral oil, and a yellow tint. The sunscreen is not scented which is always a plus. The yellow tint was not noticeable on my deep bronze skin. My skin is sensitive to many chemical products but not this one. The product blended in well into my skin both when dry and when using a moisturizing toner. There was not a white cast after giving it a few minutes to be absorbed by the skin. Most oil-based sunscreens leave my skin feeling greasy when perspiring but the Eucerin 50 was a pleasant surprise. The perspiration felt normal and not greasy.
Overall, I am very pleased with the product. It is great for active people who normally sweat away sunscreen. Eucerin 50 left my skin feeling very soft after washing it off. This is the first mineral oil-based sunscreen that I have found to be satisfactory. I believe it to be a good value at the twenty-dollar or less price range.
Warning!: It can stain your clothing. Do not store oil-based sunscreens in a hot environment like your automobile. The ingredients will separate.
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Reviewed in the United States on May 31, 2025
★★★★★ 5
I've tried MANY sunscreens. This is the best for the money you can buy.
Style: 4 Fl Oz (Pack of 1)
I have a section in my closet reserved for the graves of past sunscreens. And there are A LOT. Reasons why this is the best for price point:
1. No white cast. Maybe 2%. Yes, there is a bad a white cast when you first put it on, especially if your face is still a tad damp from washing. But it almost completely disappears within minutes. And this is coming from someone who is of Southeast Asian descent, and currently have a natural a tan this time of year. I get tan because I don't apply it every 2 hours as recommended. I only use it when I'm working out outside and the sun is blasting my face. I live where it gets very sunny.
2. NOT oily. I have super oily skin and so many of my sunscreens makes my face more of an oil slick than normal. I have matiffying ones, that work well, but it gets expensive.
3. Fragrance free! I prefer to use as many fragrance-free products as possible.
4. DOESN'T STING EYES. I'm surprised at how many "sunscreens for the face" are out there that absolutely sting my eyes into waterworks.
5. Price-point. A giant tube (for the face) for all that? It will take me a very long time to go through it. For my body, I use cheaper fragrance-free mineral sunscreens, or sunscreens I want to get rid of.
6. I use a moderate strength prescription of Tretinoin, and those who know, know that without sun protection, the skin can get burned and irritated extremely easily. Not me! And I use Tretinoin every single night.
I'm so glad Eucerin made this, I hope they never discontinue this!
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Reviewed in the United States on July 28, 2022