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Description
ATP4B Recombinant Rabbit mAb (SDT-2445-64)Product Specification Host Rabbit Antigen ATP4B Synonyms Potassium transporting ATPase subunit beta; Gastric H(+) K(+) ATPase subunit beta; Proton pump beta chain Immunogen Synthetic Peptide Location Cell membrane Accession P51164 Clone Number SDT 2445 64 Antibody Type Recombinant mAb Isotype IgG Application IHC P, IF Reactivity Hu Purification Protein A Concentration 0. 25 mg ml Conjugation Unconjugated Physical Appearance Liquid Storage Buffer PBS,
Product Specification
| Host | Rabbit |
| Antigen | ATP4B |
| Synonyms | Potassium-transporting ATPase subunit beta; Gastric H(+)/K(+) ATPase subunit beta; Proton pump beta chain |
| Immunogen | Synthetic Peptide |
| Location | Cell membrane |
| Accession | P51164 |
| Clone Number | SDT-2445-64 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | IHC-P, IF |
| Reactivity | Hu |
| Purification | Protein A |
| Concentration | 0.25 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| IHC-P | 1:1000-1:2000 | Hu |
| IF | 1:250-1:500 | Hu |
Background
ATP4B (ATPase H+/K+ transporting subunit beta) is a 291-amino-acid, heavily glycosylated transmembrane protein that forms the regulatory β-subunit of the heterodimeric gastric H+/K+-ATPase proton pump, which exchanges cytoplasmic H+ for extracellular K+ across the apical membrane of parietal cells to drive gastric acid secretion; it stabilizes and targets the catalytic α-subunit to the membrane, acts as a conformational ratchet that locks the pump in its outward-facing E2 state, and contributes to epithelial cell polarity and adhesion through an immunoglobulin-like C-terminal ectodomain .
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