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Description
Caspase 4 Activity Detection KitProduct Specification Usage Determination of pNA standard curve: 1. The 10 mM pNA standard was diluted with reaction buffer to 200, 100, 50, 25, 12. 5, 6. 25, 3. 125 M. Set the zero tube without pNA. 2. Take 100l of each concentration and add it to a 96 well plate or 100l cuvette, and measure the absorbance OD value at 405nm. 3. The OD405 value of each concentration standard tube is the x axis, and the corresponding pNA concentration is the y axis.
Product Specification
| Usage | Determination of pNA standard curve: 1. The 10 mM pNA standard was diluted with reaction buffer to 200, 100, 50, 25, 12.5, 6.25, 3.125 μM. Set the zero tube without pNA. 2. Take 100μl of each concentration and add it to a 96-well plate or 100μl cuvette, and measure the absorbance OD value at 405nm. 3. The OD405 value of each concentration standard tube is the x-axis, and the corresponding pNA concentration is the y-axis. Use Excel to make a standard curve of pNA concentration versus OD405 value. Detection of Caspase 4 enzyme activity: 1. Take out an appropriate amount of Ac-LEVD-pNA (2mM) and place it on an ice bath for later use. 2. According to the 100ul reaction system: blank control group: 40μl of detection buffer, 0μl of sample to be tested, 50μl of lysate, 10μl of Ac-LEVD-pNA (2mM); Sample group: 40μl of detection buffer, 50μl of sample to be tested, 0μl of lysate, 10μl3 of Ac-LEVD-pNA (2mM). Add Ac-LEVD-pNA (2mM) and mix well. Be careful to avoid bubbles during mixing. Incubate at 37 º C for 60-120 minutes. A405 can be measured when the color change is obvious. If the color change is not obvious, the incubation time can be appropriately extended, or even overnight. 4. The A405 of the sample minus the A405 of the blank control is the absorbance produced by pNA catalyzed by caspase 4 in the sample. By comparing the standard curves obtained in the above steps, it is possible to calculate the amount of pNA catalytically produced in the sample. |
| Theory | Based on Caspase 4, it can catalyze the substrate Leu Glu Val Asp p nitroanilide (Ac LEVD pNA) to produce yellow pNA (p-nitroaniline), which can be used to detect the activity of Caspase 4 by measuring absorbance. pNA has a maximum absorbance at 405 nm. Caspase activity was obtained by measuring pNA using visible light colorimetry. Suitable for detecting Caspase 4 activity in mammalian tissues and cells. |
| Experiment Reagent Instrument | Microplate reader or spectrophotometer |
| Synonym | caspase-4,caspase 4 |
| Description | caspase 4 Activity Detection Kit is a kit that detects caspase 4 enzyme activity or purified caspase 4 enzyme activity in cell or tissue lysates by spectrophotometry. |
| Storage Temp. | When stored at-20 °C, Ac-LEVD-pNA and pNA need to be stored in the dark. |
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4.4 ★★★★★
Based on 28 reviews
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Product Reviews
★★★★★ 5
A+ mount for 4Runners
This is the best phone mount I've ever had. Got it for my 2021 4Runner. Very strong, doesn't come loose, easy to place and remove the phone from the mount. Can't recommend enough.
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Reviewed in the United States on February 13, 2026
★★★★★ 5
High quality
Very nice and secure. This one is built to last!
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Reviewed in the United States on May 11, 2026
★★★★★ 5
Best Phone Mount EVER!
I searched the Toyota forums and consistently saw this item mentioned as one that would not move on bump roads or even over time on smooth roads. It is the best and must secure mount I have ever used. It fits perfectly on was designed not to obscure the view. I have recommended this brand to everyone that I have talked to about mounts since I got. Very easy to install also!
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Reviewed in the United States on September 15, 2025
★★★★★ 5
The fit is perfect and is a sold mounting system
Custom fit for 2022 Tacoma, fit exactly. Very sturdy mount and took seconds to install. All metal with the exception of a adjustment knob. Quality hardware. More expensive than a lot of mounts but most are cheap plastic and are not nearly as solid and sturdy as the Offroam.
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Reviewed in the United States on October 15, 2025
★★★★★ 5
FINALLY a decent phone mount!!!
I’ve tried about 5 different phone mounts in my 2018 Tacoma and ended up throwing them all away. They’re either too bulky, too weak to hold the phone, awkward or just won’t stay in place. I won’t install a holder that requires adhesive or screws into the dash (or anywhere) in my truck. This holder is the answer. Fits perfectly, doesn’t damage your vehicle. Holds the phone really solidly. This is a very sturdy mount.
It’s a bit pricy in comparison to other phone mounts out there. IMO, it’s worth every penny. Materials are far superior to your typical mount, which I’m sure drives up the manufacturing costs. Again, worth every penny. Also, it’s a small business based in the US. Love supporting our small businesses.
GREAT MOUNT!!!
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Reviewed in the United States on November 11, 2022
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