Pay in installments of $46.03 with
,
and
Shipping Estimate
USA
- USA
- CAN
- USA
- CAN
Ships within 48 hours · Estimated delivery Aug 17 - Aug 22
Promo Codes Available:
For Your Every Summer RSVP, with Code: SUMMER15
Description
Mouse ANGPTL2 ELISA KitProduct Specification Usage Experimental equipment required for the experiment: 1. Microplate reader (450nm) 2. High precision pipette and gun tips: 0. 5 10uL, 5 50uL, 20 200uL, 200 1000uL 3. 37 constant temperature box 4. Distilled water or deionized water Sample processing and requirements: Serum: Place the whole blood sample collected in the serum separation tube at room temperature for 2 hours or at 4 overnight, then centrifuge at 1000g for 20
Product Specification
| Usage |
Experimental equipment required for the experiment: 1. Microplate reader (450nm) 2. High-precision pipette and gun tips: 0.5-10uL, 5-50uL, 20-200uL, 200-1000uL 3. 37℃ constant temperature box 4. Distilled water or deionized water Sample processing and requirements: Serum: Place the whole blood sample collected in the serum separation tube at room temperature for 2 hours or at 4℃ overnight, then centrifuge at 1000×g for 20 minutes, and take the supernatant, or store the supernatant at -20℃ or -80℃, but avoid repeated freezing and thawing. Plasma: Collect the specimen using EDTA or heparin as an anticoagulant. Centrifuge the specimen at 1000 × g for 15 minutes at 2-8°C within 30 minutes of collection. The supernatant can be assayed or stored at -20°C or -80°C, but avoid repeated freezing and thawing. Tissue homogenization: Rinse the tissue with pre-chilled PBS (0.01M, pH 7.4) to remove residual blood (lysed red blood cells in the homogenate will affect the measurement results). Weigh the tissue and mince it. Add the minced tissue to the appropriate volume of PBS (generally a 1:9 weight-to-volume ratio, e.g., 1 g of tissue sample to 9 mL of PBS. The specific volume can be adjusted according to experimental needs and recorded. It is recommended to add protease inhibitors to the PBS) in a glass homogenizer and grind thoroughly on ice. To further lyse tissue cells, the homogenate can be sonicated or repeatedly frozen and thawed. Finally, centrifuge the homogenate at 5000 × g for 5-10 minutes, and the supernatant can be assayed. Cell Lysis Buffer: Gently wash adherent cells with ice-cold PBS, then trypsinize and collect cells by centrifugation at 1000×g for 5 minutes. Suspension cells can be collected directly by centrifugation. Wash collected cells three times with ice-cold PBS and resuspend in 150-200 μL of PBS per 1×10^6 cells (it is recommended to add protease inhibitors to the PBS; if the cell count is very low, reduce the PBS volume appropriately). Disrupt the cells by repeated freeze-thaw cycles or sonication. Centrifuge the extract at 1500×g for 10 minutes at 2-8°C, and remove the supernatant for analysis. Cell Culture Supernatant: Centrifuge at 1000×g for 20 minutes. Remove the supernatant for analysis or store at -20°C or -80°C, avoiding repeated freeze-thaw cycles. Other biological fluids: Centrifuge at 1000xg for 20 minutes, remove the supernatant, and test. Pre-test preparation: 1. Remove the test kit from the refrigerator 10 minutes in advance and equilibrate to room temperature. 2. Prepare the standard gradient working solution: Add 1 mL of universal diluent to the lyophilized standard, let it stand for 15 minutes to completely dissolve, then gently mix (concentration is 20 ng/mL). Then dilute to the following concentrations: 20 ng/mL, 10 ng/mL, 5 ng/mL, 2.5 ng/mL, 1.25 ng/mL, 0.625 ng/mL, 0.3125 ng/mL, and 0 ng/mL. Serial dilution method: Take 7 EP tubes and add 500 μL of universal diluent to each tube. Pipette 500 μL of the 20 ng/mL standard working solution into the first EP tube and mix thoroughly to make a 10 ng/mL standard working solution. Repeat this procedure for subsequent tubes. The last tube serves directly as a blank well; there is no need to aspirate the liquid from the penultimate tube. See the figure below for details. 3. Preparation of Biotinylated Antibody Working Solution: 15 minutes before use, centrifuge the concentrated biotinylated antibody at 1000×g for 1 minute. Dilute the 100× concentrated biotinylated antibody to a 1× working concentration using universal diluent (e.g., 10µL concentrate + 990µL universal diluent). Prepare immediately before use. 4. Prepare the enzyme conjugate working solution: 15 minutes before use, centrifuge the 100× concentrated enzyme conjugate at 1000×g for 1 minute. Dilute the 100× concentrated HRP enzyme conjugate to a 1× working concentration with universal diluent (e.g., 10 μL of concentrate + 990 μL of universal diluent). Prepare immediately. 5. Prepare the 1× wash solution: Dispense 10 mL of 20× wash solution into 190 mL of distilled water (concentrated wash solution removed from the refrigerator may crystallize; this is normal. Allow to stand at room temperature until the crystals have completely dissolved before preparing). Procedure: 1. Remove the desired strips from the aluminum foil bag after equilibration at room temperature for 10 minutes. Seal the remaining strips in a ziplock bag and return to 4°C. 2. Sample addition: Add 100 μL of sample or standard of varying concentrations to the corresponding wells. Add 100 μL of universal diluent to the blank wells. Cover with a film and incubate at 37°C for 60 minutes. (Recommendation: Dilute the sample to be tested at least 1-fold with universal diluent before adding it to the ELISA plate. This will reduce the impact of matrix effects on the test results. The sample concentration should be multiplied by the corresponding dilution factor when calculating the final sample concentration. It is recommended to run replicates for all test samples and standards.) 3. Add Biotinylated Antibody: Remove the ELISA plate and discard the liquid without washing. Add 100 μL of Biotinylated Antibody Working Solution directly to each well. Cover with a film and incubate at 37°C for 60 minutes. 4. Wash: Discard the liquid and add 300 μL of 1x Wash Solution to each well. Let stand for 1 minute, shake off the wash solution, and pat dry on absorbent paper. Repeat this process three times (a plate washer can also be used). 5. Add Enzyme Conjugate Working Solution: Add 100 μL of Enzyme Conjugate Working Solution to each well. Cover with a film and incubate at 37°C for 30 minutes. 6. Washing: Discard the liquid and wash the plate five times as in step 4. 7. Adding substrate: Add 90 μL of substrate (TMB) to each well, cover with a sealing film, and incubate at 37°C in the dark for 15 minutes. 8. Adding stop solution: Remove the ELISA plate and add 50 μL of stop solution directly to each well. Immediately measure the OD value of each well at a wavelength of 450 nm. Calculating experimental results: 1. Calculate the average OD value of the standard and sample replicates and subtract the OD value of the blank well as a correction factor. Plot the standard curve of the four-parameter logistic function on double-logarithmic graph paper, with concentration as the horizontal axis and OD value as the vertical axis. 2. If the sample OD value is higher than the upper limit of the standard curve, dilute the sample appropriately and retest. Multiply the sample concentration by the corresponding dilution factor. |
|||||||||||||||||||||||||||||||||
| Theory | This kit utilizes a double-antibody sandwich enzyme-linked immunosorbent assay (ELISA). Sample, standard, biotin-labeled detection antibody, and HRP conjugate are sequentially added to microwells pre-coated with angiopoietin-like protein 2 (ANGPTL2) capture antibody. After incubation and washing, the sample is developed using the substrate TMB. TMB converts to blue under the catalysis of HRP and to yellow under the action of acid. The intensity of the color is positively correlated with the amount of angiopoietin-like protein 2 (ANGPTL2) in the sample. The absorbance (OD) is measured at 450 nm using a microplate reader to calculate the sample concentration. | |||||||||||||||||||||||||||||||||
| Source | Mouse | |||||||||||||||||||||||||||||||||
| Synonym | Mouse Angiopoietin Like Protein 2 ELISA Kit | |||||||||||||||||||||||||||||||||
| Detection Type | Double antibody sandwich method | |||||||||||||||||||||||||||||||||
| Composition |
|
|||||||||||||||||||||||||||||||||
| Background | Angiopoietin-like protein 2 (ANGPTL2), also known as angiopoietin-related protein 2 or ARP2, is a protein encoded by the ANGPTL2 gene. It maintains tissue homeostasis by promoting adaptive inflammation and subsequent tissue remodeling. Excessive ANGPTL2 activation caused by chronic stress promotes the breakdown of tissue homeostasis caused by chronic inflammation and contributes to the development of metabolic diseases. It also plays a role in angiogenesis, tissue repair, obesity, atherosclerotic disease, and ultimately carcinogenesis. It is a secreted glycoprotein with homology to angiopoietin and can exert its effects on endothelial cells through autocrine or paracrine actions. | |||||||||||||||||||||||||||||||||
| General Notes | 1. Strictly adhere to the specified incubation time and temperature to ensure accurate results. All reagents must be at room temperature (20-25°C) before use. Refrigerate reagents immediately after use. 2. Improper plate washing may result in inaccurate results. Ensure that all liquid in the wells is aspirated thoroughly before adding substrate. Do not allow the wells to dry out during incubation. 3. Remove any residual liquid and fingerprints from the bottom of the plate, as this will affect the OD value. 4. The substrate developer solution should be colorless or very light in color. Do not use substrate solution that has turned blue. 5. Avoid cross-contamination of reagents and specimens to prevent erroneous results. 6. Avoid direct exposure to strong light during storage and incubation. 7. Do not expose any reagents to bleaching solvents or the strong fumes emitted by bleaching solvents. Any bleaching agent will destroy the biological activity of the reagents in the kit. 8. Do not use expired products, and do not mix components with different product numbers and batches. 9. Recombinant proteins from sources other than the kit may not be compatible with the antibodies in this kit and will not be recognized. 10. If there is a possibility of disease transmission, all samples should be managed properly and samples and testing devices should be handled according to prescribed procedures. |
|||||||||||||||||||||||||||||||||
| Storage Temp. | If the unopened kit is stored at 4°C, the shelf life is 6 months. | |||||||||||||||||||||||||||||||||
| Test Range | 0.31-20 ng/mL | |||||||||||||||||||||||||||||||||
| Applications | Serum, plasma, tissue homogenate, cell lysate, cell culture supernatant and other biological fluids |
Shipping Notes
- Free Standard Shipping on $100+ Orders to the USA.
- Except Preorder products are shipped in 48 hours.
- Delivery to the USA:
- Standard Shipping : 3-10 business days
- If time is of the essence, please consider selecting expedited delivery for faster service.
Exchange/Return Notes
- We offer a 30-day return/exchange service after receiving.
- Final sale items are not eligible for returns or exchanges.
- To process your return/exchange, please contact us at [email protected]
- Please click here for more details>>> Return & Exchange Policy
4.7 ★★★★★
Based on 23 reviews
Sort
Product Reviews
★★★★★ 5
We Love e.l.f.!
Color: Taupe, Size: 0.006 Ounce (Pack of 1), Color: Taupe, Size: 0.006 Ounce (Pack of 1)
I love e.l.f., and at this point I feel like half my makeup bag is from them because they keep getting it right and for a great price. I got this brow pencil in the taupe shade and the color is exactly what I was hoping for. It’s soft and natural looking without pulling too warm or making my brows look too dark and heavy. It fills everything in nicely while still looking like real brows. For reference I have blonde highlights and lighter features so if I use anything too dark it looks unnatural.
The pencil tip is small enough to do little hair-like strokes, which I love for sparse areas, but it’s also easy to blend and shade quickly when I’m getting ready in a rush. The formula has a really nice texture too. It’s not too waxy or too creamy, so it glides on smoothly without smearing everywhere. The spoolie is actually useful too and helps soften everything so my brows look natural instead of overdone.
For the price, this is such a good everyday brow pencil and definitely another e.l.f. product I’ll keep purchasing.
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on May 16, 2026
★★★★★ 5
Great for building a natural brow definition & body
Color: Taupe, Size: 0.006 Ounce (Pack of 1), Color: Taupe, Size: 0.006 Ounce (Pack of 1)
I love this eyebrow pen when I’m going for a more natural look with my everyday makeup. Great price for a great product. I brush my eyebrows out, apply with the grain of my brows and finish with another good brush throw to blend the product and it looks great. I get the color Taupe as I have dirty blonde hair, and the match is great for my brows. Quick and easy pick me up to give my brows some extra structure and definition.
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on June 4, 2026
★★★★★ 5
LOOOVEE ITTT!!!
Color: Deep Brown, Size: 0.006 Ounce (Pack of 1)
Have been using it for months now! I am in love with elf and its makeup line, everything is of high quality and works on every skin type, this pencil is soo nice, tho one disadvantage it has is it gets removed easily if u swipe your hand accidentally, other than that LOVE IT, the shape is very precise, color is very natural.
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on April 8, 2026
★★★★★ 5
Great Product!
Color: Blonde, Size: 0.006 Ounce (Pack of 1)
I've used this for several years, and am always pleased with easy it is to apply. The brush helps to keep the look natural. The price is amazing, and best yet, it's EWG Verified. I use the blond color and it's a perfect match. You can't go wrong with it.
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on May 4, 2026
★★★★★ 5
EXACTLY as advertised and MORE! HIGHLY recommended! 😊
Scent: Unscented
I've been using this product for 2 years and it works as stated. I have sensitive skin and this doesn't irritate my skin at all.
Every so often, I've asked those close to me if they've noticed any odors over the past two years and they've always replied no. I've used other brands previously that were specifically labeled as "gentle" or for "sensitive skin", which were anything but. Those were also HIGHLY scented and irritated my skin, unlike this product which is very effective without all the unnecessary chemical additives. I feel the coconut oil has soothing properties and it actually helps keep my underarm skin healthy instead of making it parched, like other brands do.
This product doesn't state that it's for sensitive skin but for me it's far more gentle than any other brand I've used. I also like that it doesn't contain any harsh anti-perspirants so my body can breathe and cool itself off properly through the natural act of sweating. The act of sweating releases toxins through your skin pores so it's not healthy to impede that process. This product supports that natural process by not containing any anti-perspirant chemicals.
Although it's labeled as unscented, science will tell you that everything in the world has a scent (even water) and this product has a very minimal wisp of a scent that's nearly imperceptible. Manufacturer's usually refer to this as a 'masking scent' so you don't notice the smell of chemicals in a product. However, this product has minimal chemicals (that's why I chose it) so there's not much to cover up and that's a good thing!
Neither I, nor anyone else that I've asked, can detect a fragrance. The ONLY time I can smell anything is when I apply it to my underarms, so the product is obviously in closer proximity to my nose. As I glide it across my skin a faint scent of a Lifesaver's pineapple flavor candy takes me immediately back to my happy childhood memories. It lasts for a few seconds and then it's gone. Could just be me but every time I use this I end up with a smile on my face. 😊
I think this happens because your underarm skin is warm so the deodorant also warms and the solidified coconut oil melts a bit as you apply it, which releases the barely-there scent. I don't smell coconut, I smell glorious pineapple! Whoever thought to make it like this is genius! For those of you who don't smell anything for those few seconds, you're missing out.
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on January 16, 2026