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Description
Mouse ERK1 ELISA KitProduct Specification Usage Experimental equipment required for the experiment: 1. Microplate reader (450nm) 2. High precision pipette and gun tips: 0. 5 10uL, 5 50uL, 20 200uL, 200 1000uL 3. 37 constant temperature box 4. Distilled water or deionized water Sample processing and requirements: 1. Serum: Place the whole blood sample collected in the serum separation tube at room temperature for 2 hours or at 4 overnight, then centrifuge at 1000g for 20
Product Specification
| Usage |
Experimental equipment required for the experiment: 1. Microplate reader (450nm) 2. High-precision pipette and gun tips: 0.5-10uL, 5-50uL, 20-200uL, 200-1000uL 3. 37℃ constant temperature box 4. Distilled water or deionized water Sample processing and requirements: 1. Serum: Place the whole blood sample collected in the serum separation tube at room temperature for 2 hours or at 4℃ overnight, then centrifuge at 1000×g for 20 minutes, and take the supernatant, or store the supernatant at -20℃ or -80℃, but avoid repeated freezing and thawing. 2. Plasma: Collect the specimen using EDTA or heparin as an anticoagulant. Centrifuge the specimen at 1000 × g for 15 minutes at 2-8°C within 30 minutes of collection. The supernatant can be assayed or stored at -20°C or -80°C, but avoid repeated freezing and thawing. 3. Tissue homogenization: Rinse the tissue with pre-chilled PBS (0.01M, pH 7.4) to remove residual blood (lysed red blood cells in the homogenate will affect the measurement results). Weigh the tissue and mince it. Add the minced tissue to the appropriate volume of PBS (generally a 1:9 weight-to-volume ratio, e.g., 1 g of tissue sample to 9 mL of PBS. The specific volume can be adjusted according to experimental needs and recorded. It is recommended to add protease inhibitors to the PBS) in a glass homogenizer and grind thoroughly on ice. To further lyse tissue cells, the homogenate can be sonicated or repeatedly frozen and thawed. Finally, centrifuge the homogenate at 5000 × g for 5-10 minutes, and the supernatant can be assayed. 4. Other biological fluids: Centrifuge at 1000xg for 20 minutes, remove the supernatant, and test. Pre-test preparation: 1. Remove the test kit from the refrigerator 10 minutes in advance and equilibrate to room temperature. 2. Prepare the standard gradient working solution: Add 1 mL of universal diluent to the lyophilized standard, let it stand for 15 minutes to completely dissolve, then gently mix (concentration is 20 ng/mL). Then dilute to the following concentrations: 20 ng/mL, 10 ng/mL, 5 ng/mL, 2.5 ng/mL, 1.25 ng/mL, 0.625 ng/mL, 0.3125 ng/mL, and 0 ng/mL. Serial dilution method: Take 7 EP tubes and add 500 μL of universal diluent to each tube. Pipette 500 μL of the 20 ng/mL standard working solution into the first EP tube and mix thoroughly to make a 10 ng/mL standard working solution. Repeat this procedure for subsequent tubes. The last tube serves directly as a blank well; there is no need to aspirate the liquid from the penultimate tube. See the figure below for details. 3. Preparation of Biotinylated Antibody Working Solution: 15 minutes before use, centrifuge the concentrated biotinylated antibody at 1000×g for 1 minute. Dilute the 100× concentrated biotinylated antibody to a 1× working concentration using universal diluent (e.g., 10µL concentrate + 990µL universal diluent). Prepare immediately before use. 4. Prepare the enzyme conjugate working solution: 15 minutes before use, centrifuge the 100× concentrated enzyme conjugate at 1000×g for 1 minute. Dilute the 100× concentrated HRP enzyme conjugate to a 1× working concentration with universal diluent (e.g., 10 μL of concentrate + 990 μL of universal diluent). Prepare immediately. 5. Prepare the 1× wash solution: Dispense 10 mL of 20× wash solution into 190 mL of distilled water (concentrated wash solution removed from the refrigerator may crystallize; this is normal. Allow to stand at room temperature until the crystals have completely dissolved before preparing). Procedure: 1. Remove the desired strips from the aluminum foil bag after equilibration at room temperature for 10 minutes. Seal the remaining strips in a ziplock bag and return to 4°C. 2. Sample addition: Add 100 μL of sample or standard of varying concentrations to the corresponding wells. Add 100 μL of universal diluent to the blank wells. Cover with a film and incubate at 37°C for 60 minutes. (Recommendation: Dilute the sample to be tested at least 1-fold with universal diluent before adding it to the ELISA plate. This will reduce the impact of matrix effects on the test results. The sample concentration should be multiplied by the corresponding dilution factor when calculating the final sample concentration. It is recommended to run replicates for all test samples and standards.) 3. Add Biotinylated Antibody: Remove the ELISA plate and discard the liquid without washing. Add 100 μL of Biotinylated Antibody Working Solution directly to each well. Cover with a film and incubate at 37°C for 60 minutes. 4. Wash: Discard the liquid and add 300 μL of 1x Wash Solution to each well. Let stand for 1 minute, shake off the wash solution, and pat dry on absorbent paper. Repeat this process three times (a plate washer can also be used). 5. Add Enzyme Conjugate Working Solution: Add 100 μL of Enzyme Conjugate Working Solution to each well. Cover with a film and incubate at 37°C for 30 minutes. 6. Washing: Discard the liquid and wash the plate five times as in step 4. 7. Adding substrate: Add 90 μL of substrate (TMB) to each well, cover with a sealing film, and incubate at 37°C in the dark for 15 minutes. 8. Adding stop solution: Remove the ELISA plate and add 50 μL of stop solution directly to each well. Immediately measure the OD value of each well at a wavelength of 450 nm. Calculating experimental results: 1. Calculate the average OD value of the standard and sample replicates and subtract the OD value of the blank well as a correction factor. Plot the standard curve of the four-parameter logistic function on double-logarithmic graph paper, with concentration as the horizontal axis and OD value as the vertical axis. 2. If the sample OD value is higher than the upper limit of the standard curve, dilute the sample appropriately and retest. Multiply the sample concentration by the corresponding dilution factor. |
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| Theory | This kit utilizes a double-antibody sandwich enzyme-linked immunosorbent assay (ELISA). Sample, standard, biotin-labeled detection antibody, and HRP conjugate are sequentially added to microwells pre-coated with an extracellular signal regulated kinase 1 (ERK1) capture antibody. After incubation and washing, the assay is developed using the substrate TMB. TMB converts to blue under the catalysis of HRP and to yellow under the action of acid. The intensity of the color is positively correlated with the amount of extracellular signal regulated kinase 1 (ERK1) in the sample. The absorbance (OD) is measured at 450 nm using a microplate reader to calculate the sample concentration. | |||||||||||||||||||||||||||||||||
| Source | Mouse | |||||||||||||||||||||||||||||||||
| Synonym | Mouse Extracellular Signal Regulated Kinase 1 ELISA Kit | |||||||||||||||||||||||||||||||||
| Detection Type | Double antibody sandwich method | |||||||||||||||||||||||||||||||||
| Composition |
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| Background | Extracellular signal-regulated kinase 1 (ERK1), also known as MAPK3, is an enzyme encoded by the MAPK3 gene. The protein encoded by this gene is a member of the mitogen-activated protein kinase (MAP kinase) family. Also known as extracellular signal-regulated kinases (ERKs), it functions in a signaling cascade that regulates various cellular processes, such as proliferation, differentiation, and cell cycle progression, in response to various extracellular signals. Activation by upstream kinases causes its translocation to the nucleus, where it phosphorylates its nuclear targets. | |||||||||||||||||||||||||||||||||
| General Notes | 1. Strictly adhere to the specified incubation time and temperature to ensure accurate results. All reagents must be at room temperature (20-25°C) before use. Refrigerate reagents immediately after use. 2. Improper plate washing may result in inaccurate results. Ensure that all liquid in the wells is aspirated thoroughly before adding substrate. Do not allow the wells to dry out during incubation. 3. Remove any residual liquid and fingerprints from the bottom of the plate, as this will affect the OD value. 4. The substrate developer solution should be colorless or very light in color. Do not use substrate solution that has turned blue. 5. Avoid cross-contamination of reagents and specimens to prevent erroneous results. 6. Avoid direct exposure to strong light during storage and incubation. 7. Do not expose any reagents to bleaching solvents or the strong fumes emitted by bleaching solvents. Any bleaching agent will destroy the biological activity of the reagents in the kit. 8. Do not use expired products, and do not mix components with different product numbers and batches. 9. Recombinant proteins from sources other than the kit may not be compatible with the antibodies in this kit and will not be recognized. 10. If there is a possibility of disease transmission, all samples should be managed properly and samples and testing devices should be handled according to prescribed procedures. |
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| Storage Temp. | If the unopened kit is stored at 4°C, the shelf life is 6 months. | |||||||||||||||||||||||||||||||||
| Test Range | 0.312-20 ng/mL | |||||||||||||||||||||||||||||||||
| Applications | Serum, plasma, tissue homogenates and other biological fluids |
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4.8 ★★★★★
Based on 14 reviews
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Product Reviews
★★★★★ 5
Nice bundle and great customer service
Size: Saros 10R/Qrevo CurvX/Edge 2 Accessories
I purchased this as an economical bundle for my Roborock Qrevo Curv. All of the parts seem to work and fit properly, which is good since I have actually had problems with the main brushes from a different maker. The only issue I ran into was that the Roborock website seemed to indicate that all of the Qrevo Curv models use the same filter size. Apparently the CurvX/Curv 2 Pro use a different filter. When I asked VIEWALL about it through Amazon messaging, they were kind enough to let me know about the difference and even offered to send me filters for my model. So you can count on excellent customer service from the company.
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Reviewed in the United States on January 7, 2026
★★★★★ 4
Affordable Roborock Qrevo CurvX Accessories That Fit Well
Size: Saros 10R/Qrevo CurvX/Edge 2 Accessories, Size: Saros 10R/Qrevo CurvX/Edge 2 Accessories
I have a Roborock Qrevo CurvX vacuum cleaner, so when I saw this replacement parts pack, I wanted to give it a try.
The box contained everything needed to carry out the maintenance schedule recommended in the Roborock app. It even included a small screwdriver, which made changing the side brush easy — a thoughtful extra touch.
I compared all of the replacement parts to the original ones and didn’t notice any major differences. Overall, the parts do seem slightly lower in quality than the originals, but not to the extent that I think it will significantly affect performance or durability.
I replaced the side brush without any issues, and it fit properly.
The mop pads were softer and fluffier than the originals and didn’t fit quite as snugly, although I don’t think this will have much impact on cleaning performance.
The new filter installed perfectly, and the vacuum bags also fit as expected.
Overall, I’m happy with this product. It’s reasonably priced and represents good value for money. I can see myself continuing to use these replacement parts in the future. I’m taking one star off due to the parts being of slightly lower quality
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Reviewed in the United States on May 13, 2026
★★★★★ 5
Value replacements
Size: Saros 10R/Qrevo CurvX/Edge 2 Accessories
As far as I can tell these are pretty solid replacements for the SAROS 10R. My original side brush was all torn up after just a few months of use so I found these replacement parts. Haven’t used the mop pads yet, but they are a similar design to the “upgraded” mop pads offered by Roborock which are superior to the standard one IMO. Everything else seems to be equal quality to the OEM parts, and at less than $12 I have no complaints on this kit.
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Reviewed in the United States on December 31, 2025
★★★★★ 1
Does not work for Qrevo Arc.
Size: Saros 10R/Qrevo CurvX/Edge 2 Accessories, Size: Saros 10R/Qrevo CurvX/Edge 2 Accessories
Filter does not fit my Qrevo Arc. Photo shows size comparison between my old (very dirty) filter and the replacement filter.
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Reviewed in the United States on April 6, 2026
★★★★★ 4
Solid replacement parts kit — just skip the mop pads
Size: Saros 10R/Qrevo CurvX/Edge 2 Accessories, Size: Saros 10R/Qrevo CurvX/Edge 2 Accessories
This kit has been a handy all-in-one refresh for my robot vacuum. The main brush, side brushes, filters, and dust bags all fit correctly on my Qrevo, slid in without fuss, and have been performing on par with the originals — suction feels unchanged, the side brushes kick debris toward the intake properly, and the filters look and seat identically to the ones they replaced. My one real complaint is the mop pads. They're noticeably thinner and flimsier than the OEM pads, don't hold water as well during a run, and left streakier lines on my floors compared to what I'm used to. I've ended up going back to the original-brand mop cloths and just using this kit for the dry-cleaning parts, which have genuinely been a good value. Four stars — great kit minus the mops.
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Reviewed in the United States on April 15, 2026