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Description
Human LOX ELISA KitProduct Specification Usage Experimental equipment required for the experiment: 1. Microplate reader (450nm) 2. High precision pipette and gun tips: 0. 5 10uL, 5 50uL, 20 200uL, 200 1000uL 3. 37 constant temperature box 4. Distilled water or deionized water Sample processing and requirements: 1. Serum: Place the whole blood sample collected in the serum separation tube at room temperature for 2 hours or at 4 overnight, then centrifuge at 1000g for 20
Product Specification
| Usage |
Experimental equipment required for the experiment: 1. Microplate reader (450nm) 2. High-precision pipette and gun tips: 0.5-10uL, 5-50uL, 20-200uL, 200-1000uL 3. 37℃ constant temperature box 4. Distilled water or deionized water Sample processing and requirements: 1. Serum: Place the whole blood sample collected in the serum separation tube at room temperature for 2 hours or at 4℃ overnight, then centrifuge at 1000×g for 20 minutes, and take the supernatant, or store the supernatant at -20℃ or -80℃, but avoid repeated freezing and thawing. 2. Plasma: Collect the specimen using EDTA or heparin as an anticoagulant. Centrifuge the specimen at 1000 × g for 15 minutes at 2-8°C within 30 minutes of collection. The supernatant can be assayed or stored at -20°C or -80°C, but avoid repeated freezing and thawing. 3. Tissue homogenization: Rinse the tissue with pre-chilled PBS (0.01M, pH 7.4) to remove residual blood (lysed red blood cells in the homogenate will affect the measurement results). Weigh the tissue and mince it. Add the minced tissue to the appropriate volume of PBS (generally a 1:9 weight-to-volume ratio, e.g., 1 g of tissue sample to 9 mL of PBS. The specific volume can be adjusted according to experimental needs and recorded. It is recommended to add protease inhibitors to the PBS) in a glass homogenizer and grind thoroughly on ice. To further lyse tissue cells, the homogenate can be sonicated or repeatedly frozen and thawed. Finally, centrifuge the homogenate at 5000 × g for 5-10 minutes, and the supernatant can be assayed. 4. Cell Lysis Buffer: Gently wash adherent cells with ice-cold PBS, then trypsinize and collect cells by centrifugation at 1000×g for 5 minutes. Suspension cells can be collected directly by centrifugation. Wash collected cells three times with ice-cold PBS and resuspend in 150-200 μL of PBS per 1×10^6 cells (it is recommended to add protease inhibitors to the PBS; if the cell count is very low, reduce the PBS volume appropriately). Disrupt the cells by repeated freeze-thaw cycles or sonication. Centrifuge the extract at 1500×g for 10 minutes at 2-8°C, and remove the supernatant for analysis. 5. Cell Culture Supernatant: Centrifuge at 1000×g for 20 minutes. Remove the supernatant for analysis or store at -20°C or -80°C, avoiding repeated freeze-thaw cycles. 6. Other biological fluids: Centrifuge at 1000xg for 20 minutes, remove the supernatant, and test. Pre-test preparation: 1. Remove the test kit from the refrigerator 10 minutes in advance and equilibrate to room temperature. 2. Prepare the standard gradient working solution: Add 1 mL of universal diluent to the lyophilized standard, let it stand for 15 minutes to completely dissolve, then gently mix (concentration is 10 ng/mL). Then dilute to the following concentrations: 10 ng/mL, 5 ng/mL, 2.5 ng/mL, 1.25 ng/mL, 0.625 ng/mL, 0.3125 ng/mL, 0.15625 ng/mL, and 0 ng/mL. Serial dilution method: Take seven EP tubes and add 500uL of universal diluent to each. Pipette 500uL of the 10ng/mL standard working solution into the first EP tube and mix thoroughly to make a 5ng/mL standard working solution. Repeat this procedure for subsequent tubes. The last tube serves as a blank well; there is no need to pipette liquid from the penultimate tube. See the figure below for details. 3. Preparation of biotinylated detection antibody working solution: Centrifuge the concentrated biotinylated antibody at 1000×g for 1 minute 15 minutes before use. Dilute the 100× concentrated biotinylated antibody to a 1× working concentration with universal diluent (e.g., 10uL concentrate + 990uL universal diluent). Prepare and use immediately. 4. Prepare the enzyme conjugate working solution: 15 minutes before use, centrifuge the 100× concentrated enzyme conjugate at 1000×g for 1 minute. Dilute the 100× concentrated HRP enzyme conjugate to a 1× working concentration with universal diluent (e.g., 10 μL of concentrate + 990 μL of universal diluent). Prepare immediately. 5. Prepare the 1× wash solution: Dispense 10 mL of 20× wash solution into 190 mL of distilled water (concentrated wash solution removed from the refrigerator may crystallize; this is normal. Allow to stand at room temperature until the crystals have completely dissolved before preparing). Procedure: 1. Remove the desired strips from the aluminum foil bag after equilibration at room temperature for 10 minutes. Seal the remaining strips in a ziplock bag and return to 4°C. 2. Sample addition: Add 100 μL of sample or standard of varying concentrations to the corresponding wells. Add 100 μL of universal diluent to the blank wells. Cover with a film and incubate at 37°C for 60 minutes. (Recommendation: Dilute the sample to be tested at least 1-fold with universal diluent before adding it to the ELISA plate. This will reduce the impact of matrix effects on the test results. The sample concentration should be multiplied by the corresponding dilution factor when calculating the final sample concentration. It is recommended to run replicates for all test samples and standards.) 3. Add Biotinylated Antibody: Remove the ELISA plate and discard the liquid without washing. Add 100 μL of Biotinylated Antibody Working Solution directly to each well. Cover with a film and incubate at 37°C for 60 minutes. 4. Wash: Discard the liquid and add 300 μL of 1x Wash Solution to each well. Let stand for 1 minute, shake off the wash solution, and pat dry on absorbent paper. Repeat this process three times (a plate washer can also be used). 5. Add Enzyme Conjugate Working Solution: Add 100 μL of Enzyme Conjugate Working Solution to each well. Cover with a film and incubate at 37°C for 30 minutes. 6. Washing: Discard the liquid and wash the plate five times as in step 4. 7. Adding substrate: Add 90 μL of substrate (TMB) to each well, cover with a sealing film, and incubate at 37°C in the dark for 15 minutes. 8. Adding stop solution: Remove the ELISA plate and add 50 μL of stop solution directly to each well. Immediately measure the OD value of each well at a wavelength of 450 nm. Calculating experimental results: 1. Calculate the average OD value of the standard and sample replicates and subtract the OD value of the blank well as a correction factor. Plot the standard curve of the four-parameter logistic function on double-logarithmic graph paper, with concentration as the horizontal axis and OD value as the vertical axis. 2. If the sample OD value is higher than the upper limit of the standard curve, dilute the sample appropriately and retest. Multiply the sample concentration by the corresponding dilution factor. |
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| Theory | This kit uses a double-antibody sandwich enzyme-linked immunosorbent assay (ELISA). Sample, standard, biotin-labeled detection antibody, and HRP conjugate are sequentially added to microwells pre-coated with a lysyl oxidase (LOX) capture antibody. After incubation and washing, the sample is developed using the substrate TMB. TMB is converted to blue under the catalysis of HRP and to yellow under the action of acid. The intensity of the color is positively correlated with the amount of lysyl oxidase (LOX) in the sample. The absorbance (OD) is measured at 450 nm using a microplate reader to calculate the sample concentration. | |||||||||||||||||||||||||||||||||
| Source | Human | |||||||||||||||||||||||||||||||||
| Synonym | Human Lysyl Oxidase ELISA Kit | |||||||||||||||||||||||||||||||||
| Detection Type | Double antibody sandwich method | |||||||||||||||||||||||||||||||||
| Composition |
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| Background | Lysine oxidase (LOX), also known as protein lysine 6-oxidase, is encoded by the LOX gene. It catalyzes the conversion of lysine molecules into highly reactive aldehydes, which form cross-links in extracellular matrix proteins. Its inhibition can cause osteoporosis, but its upregulation by tumor cells can also promote metastasis, leading to malignant and cancerous transformation of existing tumors. | |||||||||||||||||||||||||||||||||
| General Notes | 1. Strictly adhere to the specified incubation time and temperature to ensure accurate results. All reagents must be at room temperature (20-25°C) before use. Refrigerate reagents immediately after use. 2. Improper plate washing may result in inaccurate results. Ensure that all liquid in the wells is aspirated thoroughly before adding substrate. Do not allow the wells to dry out during incubation. 3. Remove any residual liquid and fingerprints from the bottom of the plate, as this will affect the OD value. 4. The substrate developer solution should be colorless or very light in color. Do not use substrate solution that has turned blue. 5. Avoid cross-contamination of reagents and specimens to prevent erroneous results. 6. Avoid direct exposure to strong light during storage and incubation. 7. Do not expose any reagents to bleaching solvents or the strong fumes emitted by bleaching solvents. Any bleaching agent will destroy the biological activity of the reagents in the kit. 8. Do not use expired products, and do not mix components with different product numbers and batches. 9. Recombinant proteins from sources other than the kit may not be compatible with the antibodies in this kit and will not be recognized. 10. If there is a possibility of disease transmission, all samples should be managed properly and samples and testing devices should be handled according to prescribed procedures. |
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| Storage Temp. | If the unopened kit is stored at 4°C, the shelf life is 6 months. | |||||||||||||||||||||||||||||||||
| Test Range | 0.156-10 ng/mL | |||||||||||||||||||||||||||||||||
| Applications | Serum, plasma, tissue homogenate, cell lysate, cell culture supernatant and other biological fluids |
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4.5 ★★★★★
Based on 27 reviews
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Product Reviews
★★★★★ 5
Love this soap
This is a wonderful zinc soap. Cleans very well and smells great. No heavy perfume smell.
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on December 1, 2025
★★★★★ 5
great for kids with dry skin!
Scent: Fragrance Free, Size: 2 Pack - 3.5 Ounce & 8 Ounce
This ointment has been a great find for sensitive baby skin. We started using it for dry patches and mild eczema, and it made a noticeable difference within a few days. The texture is rich and soothing without feeling overly greasy, and it absorbs well enough that it doesn’t leave everything sticky.
I really appreciate that it’s fragrance-free and made without steroids—perfect for everyday use, especially on delicate skin. We’ve used it on everything from dry cheeks to minor irritation, and it’s been consistently gentle and effective.
The 2-pack is a nice bonus since we keep one at home and one in the diaper bag. A little goes a long way, so the jars last longer than expected.
The only downside is the price—it’s a bit more expensive than some alternatives—but for the quality and peace of mind, it feels worth it.
Overall, I’d definitely recommend this for parents looking for a reliable, gentle solution for dry or sensitive skin.
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Reviewed in the United States on May 3, 2026
★★★★★ 5
Only thing that worked! So thankful!
Scent: Fragrance Free, Size: 2 Pack - 8 Ounce
I can’t believe I’m going to do this, but I’m writing a review and a long one at that! My baby began having skin issues around 5weeks old. Which I believe was from washing her with Honest baby soap(but I believe any soap would have been a problem)- up until then I had only used a wash cloth and water. But that week I washed her twice with the soap and that weekend my son had back to back soccer games it was 90degeee weather and my baby got sweaty. Then after that her skin just seemed to have problem after problem! I didn’t know if it was heat rash, eczema, or baby acne or combination? So no more soap and returned to water and started putting breastmilk and tallow on her which really helped with the redness. but her skin still felt like sand paper and would easily get inflamed if warm or sweaty. Around 8weeks of age she started getting cradle cap behind and on her ears and her eyebrows, then mildly on her head ugh. And she was VERY itchy and would be itching her head all night. So I ordered MuStella shampoo and the Frida cradle cap scrubby and brush set. After 2-3 days my babies head had red bumps, very inflamed and still very itchy. It said to wash everyday and remove the scales but I just couldn’t keep doing that. I would slather tallow on my babies head after washing and I returned to just rinsing with water as that seemed to help. I would also gently use the comb because her hair would be crazy after applying cream to scalp. This also helped to further distribute the cream and clean out some dry flakes from scalp- no need to scrub with that scrubber. But she was still itchy. So that’s when I decided to add the colloidal oatmeal for the sleepless nights itching- which this has 1% as an active ingredient. The price was definitely not appealing but I got it. After 1 day of using this it was like my babies skin was completely transformed!!! Even her sandpaper feeling face got clear and smooth feeling again! All day I was just so thankful and happy to have my babies smooth skin back after weeks of battling skin problem after skin problem. I really think it had to do with the soap in the first place, maybe effected natural skin barrier. Which if you research skin ph and barrier a lot of things will do that. What really washes dirt away is rubbing and water. I could kick myself for even using the the soap on my babies precious skin.
So what worked for us was not using soaps and just washing with water, slather on tallow WHILE SKIN IS STILL WET, this locks in the moister. Water is moisture and all lotion and creams do are lock in the moisture so you want your skin wet before applying creams. Creams help slow down transepidermal water loss. Which is another reason to protect your skin barrier as it has ability to retain moisture better. So we washed with water, applied whipped tallow while still wet and this colloidal oatmeal ointment on top of that.
I can hardly believe the overnight difference! I almost never ever write a review but just really wanted to share our story if it may help others. I usually do not buy expensive products like this, but I’m glad I tried- it worked AMAZINGLY for my baby- she is so much more comfortable!!! I’m so thankful!
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Reviewed in the United States on November 5, 2024
★★★★★ 5
Great for Dry Skin, Works Fast!
Scent: Fragrance Free, Size: 2 Pack - 3.5 Ounce & 8 Ounce
10/10 would recommend. This is heavy duty lotion, cream, but it works for my baby in 1-2 uses if he has dry skin or a rash. It is worth the cost in my opinion, only want to put on a small amount at a time anyway it is very thick and a little goes a very long way.
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Reviewed in the United States on May 13, 2026
★★★★★ 5
It’s expensive for a reason, this stuff works! Even better than the steroids
Scent: Fragrance Free, Size: 2 Pack - 3.5 Ounce & 8 Ounce
It’s expensive for a reason! This stuff works, my 4 mo old has been struggling with eczema for 2 months now and we have been to the ER twice due to infections, I’ve taken everything out of my diet since he’s breastfeeding, we’ve tried every other cream and routine out there. Up until just a few days ago when we ordered Tubby Todd’s his cheeks were constantly raw and weeping. After just 3 days of use with Tubby Todd’s he is finally getting some reprieve and we are finally getting SLEEP (before we would have to hold his hands down manually all night so he wouldn’t itch himself raw) this cream is even better than the steroids, you won’t regret purchasing even at the high price point.
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Reviewed in the United States on April 27, 2026