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Description
Human EXT2 ELISA KitProduct Specification Usage Experimental equipment required for the experiment: 1. Microplate reader (450nm) 2. High precision pipette and gun tips: 0. 5 10uL, 5 50uL, 20 200uL, 200 1000uL 3. 37 constant temperature box 4. Distilled water or deionized water Sample processing and requirements: Serum: Place the whole blood sample collected in the serum separation tube at room temperature for 2 hours or at 4 overnight, then centrifuge at 1000g for 20
Product Specification
| Usage |
Experimental equipment required for the experiment: 1. Microplate reader (450nm) 2. High-precision pipette and gun tips: 0.5-10uL, 5-50uL, 20-200uL, 200-1000uL 3. 37℃ constant temperature box 4. Distilled water or deionized water Sample processing and requirements: Serum: Place the whole blood sample collected in the serum separation tube at room temperature for 2 hours or at 4℃ overnight, then centrifuge at 1000×g for 20 minutes, and take the supernatant, or store the supernatant at -20℃ or -80℃, but avoid repeated freezing and thawing. Plasma: Collect specimens using EDTA or heparin as an anticoagulant and centrifuge at 1000×g for 15 minutes at 2-8°C within 30 minutes of collection. Remove the supernatant for testing, or store at -20°C or -80°C, but avoid repeated freezing and thawing. Cell culture supernatant: Centrifuge at 1000×g for 20 minutes. Remove the supernatant for testing, or store at -20°C or -80°C, but avoid repeated freezing and thawing. Pre-Test Preparation: 1. Remove the test kit from the refrigerator 10 minutes in advance and equilibrate to room temperature. 2. Prepare a gradient standard working solution: Add 1 mL of universal diluent to the lyophilized standard. Let stand for 15 minutes to completely dissolve, then gently mix (concentration 10 ng/mL). Then dilute to the following concentrations: 10 ng/mL, 5 ng/mL, 2.5 ng/mL, 1.25 ng/mL, 0.625 ng/mL, 0.3125 ng/mL, 0.15625 ng/mL, and 0 ng/mL. Serial dilution method: Take seven EP tubes and add 500 μL of universal diluent to each tube. Pipette 500 μL of the 10 ng/mL standard working solution into the first EP tube and mix thoroughly to make a 5 ng/mL standard working solution. Repeat this procedure for subsequent tubes. The last tube serves as a blank well; there is no need to pipette liquid from the penultimate tube. See the figure below for details. 3. Preparation of biotinylated detection antibody working solution: Centrifuge the concentrated biotinylated antibody at 1000×g for 1 minute 15 minutes before use. Dilute the 100× concentrated biotinylated antibody to a 1× working concentration with universal diluent (e.g., 10uL concentrate + 990uL universal diluent). Prepare and use immediately. 4. Prepare the enzyme conjugate working solution: 15 minutes before use, centrifuge the 100× concentrated enzyme conjugate at 1000×g for 1 minute. Dilute the 100× concentrated HRP enzyme conjugate to a 1× working concentration with universal diluent (e.g., 10 μL of concentrate + 990 μL of universal diluent). Prepare immediately. 5. Prepare the 1× wash solution: Dispense 10 mL of 20× wash solution into 190 mL of distilled water (concentrated wash solution removed from the refrigerator may crystallize; this is normal. Allow to stand at room temperature until the crystals have completely dissolved before preparing). Procedure: 1. Remove the desired strips from the aluminum foil bag after equilibration at room temperature for 10 minutes. Seal the remaining strips in a ziplock bag and return to 4°C. 2. Sample addition: Add 100 μL of sample or standard of varying concentrations to the corresponding wells. Add 100 μL of universal diluent to the blank wells. Cover with a film and incubate at 37°C for 60 minutes. (Recommendation: Dilute the sample to be tested at least 1-fold with universal diluent before adding it to the ELISA plate. This will reduce the impact of matrix effects on the test results. The sample concentration should be multiplied by the corresponding dilution factor when calculating the final sample concentration. It is recommended to run replicates for all test samples and standards.) 3. Add Biotinylated Antibody: Remove the ELISA plate and discard the liquid without washing. Add 100 μL of Biotinylated Antibody Working Solution directly to each well. Cover with a film and incubate at 37°C for 60 minutes. 4. Wash: Discard the liquid and add 300 μL of 1x Wash Solution to each well. Let stand for 1 minute, shake off the wash solution, and pat dry on absorbent paper. Repeat this process three times (a plate washer can also be used). 5. Add Enzyme Conjugate Working Solution: Add 100 μL of Enzyme Conjugate Working Solution to each well. Cover with a film and incubate at 37°C for 30 minutes. 6. Washing: Discard the liquid and wash the plate five times as in step 4. 7. Adding substrate: Add 90 μL of substrate (TMB) to each well, cover with a sealing film, and incubate at 37°C in the dark for 15 minutes. 8. Adding stop solution: Remove the ELISA plate and add 50 μL of stop solution directly to each well. Immediately measure the OD value of each well at a wavelength of 450 nm. Calculating experimental results: 1. Calculate the average OD value of the standard and sample replicates and subtract the OD value of the blank well as a correction factor. Plot the standard curve of the four-parameter logistic function on double-logarithmic graph paper, with concentration as the horizontal axis and OD value as the vertical axis. 2. If the sample OD value is higher than the upper limit of the standard curve, dilute the sample appropriately and retest. Multiply the sample concentration by the corresponding dilution factor. |
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| Theory | This kit uses a double-antibody sandwich enzyme-linked immunosorbent assay (ELISA). Sample, standard, biotin-labeled detection antibody, and HRP conjugate are sequentially added to microwells pre-coated with an exostosin 2 (EXT2) capture antibody. After incubation and washing, the sample is developed using the substrate TMB. TMB is converted to blue by peroxidase (HRP) catalysis and to yellow by acid. The intensity of the color is positively correlated with the amount of exostosin 2 (EXT2) in the sample. The absorbance (OD) is measured at 450 nm using a microplate reader to calculate the sample concentration. | |||||||||||||||||||||||||||||||||
| Source | Human | |||||||||||||||||||||||||||||||||
| Synonym | Human Exostosin2 ELISA Kit | |||||||||||||||||||||||||||||||||
| Detection Type | Double antibody sandwich method | |||||||||||||||||||||||||||||||||
| Composition |
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| Background | Exostosin2 is a protein-coding gene. Disorders associated with EXT2 include seizures, scoliosis, macrocephaly/microcephaly syndrome, and exostosis, multiplex, type I. Pathways associated with this gene include glycosaminoglycan metabolism and metabolism. Gene Ontology (GO) annotations associated with this gene include protein homodimerization activity and glycosyltransferase activity. An important homologue of this gene is EXTL3. | |||||||||||||||||||||||||||||||||
| General Notes | 1. Strictly adhere to the specified incubation time and temperature to ensure accurate results. All reagents must be at room temperature (20-25°C) before use. Refrigerate reagents immediately after use. 2. Improper plate washing may result in inaccurate results. Ensure that all liquid in the wells is aspirated thoroughly before adding substrate. Do not allow the wells to dry out during incubation. 3. Remove any residual liquid and fingerprints from the bottom of the plate, as this will affect the OD value. 4. The substrate developer solution should be colorless or very light in color. Do not use substrate solution that has turned blue. 5. Avoid cross-contamination of reagents and specimens to prevent erroneous results. 6. Avoid direct exposure to strong light during storage and incubation. 7. Do not expose any reagents to bleaching solvents or the strong fumes emitted by bleaching solvents. Any bleaching agent will destroy the biological activity of the reagents in the kit. 8. Do not use expired products, and do not mix components with different product numbers and batches. 9. Recombinant proteins from sources other than the kit may not be compatible with the antibodies in this kit and will not be recognized. 10. If there is a possibility of disease transmission, all samples should be managed properly and samples and testing devices should be handled according to prescribed procedures. |
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| Storage Temp. | If the unopened kit is stored at 4°C, the shelf life is 6 months. | |||||||||||||||||||||||||||||||||
| Test Range | 0.156-10 ng/mL | |||||||||||||||||||||||||||||||||
| Applications | Serum, plasma, cell culture supernatant |
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4.4 ★★★★★
Based on 14 reviews
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Product Reviews
★★★★★ 4
Supportive and Comfortable but Size Down!
Size: X-Large, Color: Navy
Very supportive for the big chested girlies! Material is comfortable and sweat wicking. I’d recommend sizing down for a snug fit.
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Reviewed in the United States on January 25, 2026
★★★★★ 5
Nice fabric, comfortable, easy maintenance
Special Size: 27.5" Full Length, Size: Large, Color: Taupe, Special Size: 27.5" Full Length, Size: Large, Color: Taupe
I ordered a medium and they’re actually a size large. And they are just too large. I should’ve gotten the small, as recommended I thought I was ordering medium and I think I would’ve been OK with a medium, but where I usually wear a small and they sent me a large. It was just ridiculously large. They are lightweight and they are warm. I’m a senior and I’m thin so I get cold very easily especially in early morning and evening, so I like to wear them in the morning for my walks/yoga , they also work nicely under a longish skirt so it’s not so sheer… will definitely get more but will go with the small size. Just for reference, I’m 5 foot seven I usually wear a small or a size 5/6 usa size so these were too large, particularly in the waist just overall they were too large, but I think even if I got a small size they’re still gonna be kind of large in the stomach. I have an hourglass shape just for reference, but they are very comfortable, and I love the fabric. I am keeping them because I like the fabric and I really need them. I just will not wear them out of the house right now because they’re not fitting properly but next go round we’ll get the right size. Price was right they were having a sale so it was a bit of an impulse buy. But hello it was only 10 bucks! so that’s why it’s not worth sending it back for me. Overall, very nice quality and would recommend. Cats like the fabric too! 😸
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Reviewed in the United States on May 4, 2026
★★★★★ 5
Buttery soft and flattering fit
Special Size: 27.5" Full Length with Pockets, Size: Small, Color: Taupe
I love these leggings! They are buttery soft (as advertised) and have a very flattering fit. Also the grey/beige color is really pretty. About to buy another pair in black! Highly recommend!
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Reviewed in the United States on May 17, 2026
★★★★★ 5
Not for people over 5'6"
Special Size: 27.5" Full Length with Pockets, Size: XX-Large, Color: Slate Grey
These are definitely designed for shorter people. They get 5* because they're still low cost and decent quality on fabric. Just have to pull the waist up because the calfs are not designed to be worn as capris and pull the entire leggings down. If it sized up one more then they'd just fall down because of the waist.
So waistband fits as expected. They're just also for shorter people than me.
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Reviewed in the United States on March 28, 2026
★★★★★ 3
"Heather" and Non-Heather Colors are Not the same Fit & Material
Special Size: 27.5" Full Length, Size: Large, Color: Space Dye Slate
I bought these in one of the "heathered" colors and loved how soft they were. They are really comfortable and stretchy, thick enough that they can be worn on their own. They are not a perfect fit, but fit well enough and I can overlook this because of how smooth they feel.
I wanted to try other colors and only realized after purchasing that the non-heathered colors were a different material. I was able to return them but they should probably be listed as a separate item. They have an entirely different fit and texture and I would not recommend.
Five stars for the heather colors, one star for the non-heather version.
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Reviewed in the United States on April 15, 2026
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