SKU: 64689170607

Unione ®️ TR-FRET Human PD1/PD-L1 Binding Kit

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Description

Unione ®️ TR-FRET Human PD1/PD-L1 Binding KitProduct Specification Host Human Stability & Storage 80 Background The assay kit utilizes homogeneous time resolved fluorescence (TR FRET) technology to measure the interaction between PD1 and PD L1. This method enables simple, rapid, and high throughput screening of inhibitors and antibody blockers. As shown in the figure, the interaction between PD1 and PD L1 is detected using an Eu labeled anti Tag1 antibody (TR FRET donor) and an Ac labeled anti

Product Specification


Host Human
Stability & Storage

-80℃

Background

The assay kit utilizes homogeneous time-resolved fluorescence (TR-FRET) technology to measure the interaction between PD1 and PD-L1. This method enables simple, rapid, and high-throughput screening of inhibitors and antibody blockers.

As shown in the figure, the interaction between PD1 and PD-L1 is detected using an Eu-labeled anti-Tag1 antibody (TR-FRET donor) and an Ac-labeled anti-Tag2 antibody (TR-FRET acceptor). The binding of PD1 and PD-L1 brings the donor and acceptor antibodies into proximity, allowing the excitation of the donor antibody to trigger fluorescence resonance energy transfer (FRET) to the acceptor antibody, resulting in a specific emission signal at 665 nm. Pembrolizumab, as a positive control, blocks the interaction between PD1 and PD-L1, preventing FRET signal generation. The stronger the blocking effect of the screened drug on the PD1/PD-L1 interaction, the lower the signal. The specific signal is proportional to the extent of the PD1/PD-L1 interaction. This homogeneous assay is simple to perform and requires no washing steps.

Components

Component

Concentration

100T

500T

2500T

10000T

Storage Temperature

Pembrolizumab

5μM

5μL

10μL

50μL

200μL

-80℃

Tag1-PD-L1 protein

100×

5μL

20μL

100μL

400μL

-80℃

Tag2- PD1 protein

100×

5μL

20μL

100μL

400μL

-80℃

Anti-Tag1 Eu antibody

50×

10μL

50μL

250μL

1000μL

-80℃

Anti-Tag2 Ac antibody

12.5×

40μL

200μL

1000μL

4000μL

-80℃

Detection buffer

10×

400μL

2mL

10mL

40mL

-80℃


Note: Aliquot immediately upon first thaw and store at the recommended temperature. Avoid storage after dilution and repeated freeze-thaw cycles.

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Exchange/Return Notes
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SKU: 64689170607

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Evi
Alexandria, US
★★★★★ 5
Cooling pillows
Size: Queen (Pack of 2)
I was skeptical with these pillows. I received the pillows compressed. I followed the directions and within 24 hours, I had 2 beautiful fluffy cooling pillows. I was also skeptical about the cooling techniques, i was happily surprised. With the support of my neck, the comfort of the pillows. I will be getting 2 more
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Reviewed in the United States on June 1, 2026
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Jennifer
Whiting, US
★★★★★ 5
Awesome Pillow!!!
Size: Queen, Number of Items: 1
This pillow is Amazing!!! So Great to sleep on. I finally can get a good night's sleep!!! Its very soft and comfortable. I also haven’t gotten up with a stiff neck in the morning either!!!
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Reviewed in the United States on May 16, 2026
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Verified Purchase
Adam Rigdon
Port Orchard, US
★★★★★ 5
Love them!!
Size: Queen, Number of Items: 2, Size: Queen, Number of Items: 2
Ok I normally don't leave reviews but I had to on this pillow. I was looking forever for a pillow and was nervous about buying one because you know how that usually goes when it comes to buying pillows. They arrived compressed but when you unwrap them they blow up to be a heavy duty pillow. Firm but not too firm. I don't like super soft pillows that you can roll up in a ball with one hand, I like a pillow that gives support and these do just that. They are cooling as well they are pretty for a pillow with the unique design. If you are in the market for a pillow and reading this THEN BUY THIS PILLOW & come back to tell me thank you. I promise you will NOT be disappointed. No I didn't get paid to say any of this. I wanted to give a honest review where it deserves one! You will not be disappointed! Thanks for the fast over night shipping as well. Not to mention the pricing is on point. I can say right now you will not find 2 pillows of this quality & price.
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Reviewed in the United States on April 1, 2026
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Verified Purchase
KEM
Pawtucket, US
★★★★★ 5
Perfect pillows
Size: Queen, Number of Items: 2
Soft, but durable. Comfortable and cool during sleep.
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Reviewed in the United States on May 17, 2026
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James P
Charlottesville, US
★★★★★ 4
Best pillow I’ve ever tried—hands down!
Size: Queen, Number of Items: 2
I’ve gone through a bunch of pillows over the years, but this BETU Cooling Pillow is by far the best one I’ve ever used. I sleep in literally every position—on my back, stomach, and sides—and this pillow works perfectly no matter how I’m laying. It really does have a cooling effect, which I love as a hot sleeper. Just make sure your room is cool too, and it makes a big difference. It came vacuum-packed, but it fully expanded within just a few hours. I was able to sleep on it the same day it arrived, and it was super comfy right from the start. I also washed and dried the pillowcase before using it, and it came out looking and feeling great—no shrinking or damage at all. Honestly, I look forward to going to bed every night now just because of this pillow. It’s supportive, comfortable, and totally worth it!
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Reviewed in the United States on February 21, 2026

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