Pay in installments of $35.75 with
,
and
Shipping Estimate
USA
- USA
- CAN
- USA
- CAN
Ships within 48 hours · Estimated delivery Aug 13 - Aug 18
Promo Codes Available:
For Your Every Summer RSVP, with Code: SUMMER15
Description
Annexin V-APC/7-AAD apoptosis analysis kitProduct Specification Usage 1. Instrument parameter adjustment 1. Collect 1 106 3106The cells were washed twice by centrifugation with pre cooled PBS and the supernatant was discarded. 2. Add 500uL of Apoptosis Positive Control Solution to resuspend and incubate on ice for 30 minutes. 3. Wash by centrifugation with pre cooled PBS and discard the supernatant. 4. Add an appropriate amount of pre cooled 1 Binding Buffer to resuspend, and add the same
Product Specification
| Usage | 1. Instrument parameter adjustment 1. Collect 1 × 106-3×106The cells were washed twice by centrifugation with pre-cooled PBS and the supernatant was discarded. 2. Add 500uL of Apoptosis Positive Control Solution to resuspend and incubate on ice for 30 minutes. 3. Wash by centrifugation with pre-cooled PBS and discard the supernatant. 4. Add an appropriate amount of pre-cooled 1 × Binding Buffer to resuspend, and add the same number of untreated viable cells to mix with it. Add pre-cooled 1 × Binding Buffer to replenish to 1.5 mL, and divide it into three tubes equally, of which one tube is a blank control tube and two tubes are single dye tubes. 5. Add 5 uL of Annexin V-APC or 10 uL of 7-AAD to the single stain tube and incubate for 5 minutes at room temperature and protect from light. 6. On the flow cytometer, the voltages of FSC, SSC and fluorescence channel were adjusted with a blank tube, and under this voltage condition, the fluorescence was adjusted with a single staining tube Compensation of channels. Note:Certain processes of treating adherent cells as single cells cause cell membrane damage, resulting in false positives for Annexin V. Therefore, optimization is needed. It is recommended to treat adherent cells with enzymes that are more gentle on cells, such as Accutase. 2. Sample testing 1. Induce apoptosis according to the experimental protocol. 2. Centrifuge washing with pre-cooled PBS and collect 1-10 × 105Cells (including cells in the culture supernatant). Dilute 5 × Binding Buffer into 1 × working solution with double distilled water, and take 500 uL of 1 × Binding Buffer to resuspend the cells. 3. Add 5 uL of Annexin V-APC and 10 uL of 7-AAD per tube. 4. After gently vortex and mix well, incubate at room temperature and protect from light for 5 minutes. 5. Get on the computer for analysis. |
|||||||||||||||
| Beilstein | 0 | |||||||||||||||
| Synonym | Annexin V-APC/7-AAD Apoptosis Analysis Kit | |||||||||||||||
| Description |
Annexin V (or Annexin A5) is a member of the annexin family of intracellular proteins that binds to phosphatidylserine (PS) in a calcium-dependent manner. PS is present in the inner lining of the plasma membrane of normal cells, but in the early stage of apoptosis, membrane asymmetry is lost and PS translocates to the cell surface. Fluorescently Labeled Annexin V Kit Components:
|
|||||||||||||||
| PubChem CID | 0 | |||||||||||||||
| General Notes | 1. Please read the instructions carefully before using this product. This product is for scientific research only, not diagnostic. 2. For your safety and health, please wear experimental protective clothing, gloves, masks and other necessary protective equipment. |
|||||||||||||||
| Storage Temp. | Store at 2-8 °C, do not freeze, shelf life 1 year. |
Shipping Notes
- Free Standard Shipping on $100+ Orders to the USA.
- Except Preorder products are shipped in 48 hours.
- Delivery to the USA:
- Standard Shipping : 3-10 business days
- If time is of the essence, please consider selecting expedited delivery for faster service.
Exchange/Return Notes
- We offer a 30-day return/exchange service after receiving.
- Final sale items are not eligible for returns or exchanges.
- To process your return/exchange, please contact us at [email protected]
- Please click here for more details>>> Return & Exchange Policy
4.6 ★★★★★
Based on 20 reviews
Sort
Product Reviews
★★★★★ 4
A quick and interesting read. It may help you broaden your horizons with relationships!
There is no real difference in terms of what you will take away from this book if you already read the original. This book gives you some manly homework, some insight into conflicts with perspectives that I did not think of. Suffice it to say, reading this book helped me grow as a person and helped with my relationship. You come out on top as a man since this book helps keep your cool.
Basically what I'm trying to say is: If you never read any Love Language book and you want a more man-centric read. Get this and live it.
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on September 11, 2017
★★★★★ 5
Cute
A great book about how we all have different love languages.
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on January 9, 2026
★★★★★ 5
Bought as a gift
The young man is using the book to understand that we all have different love languages and I watch as he take interest in the content of the book. I gave both my daughters a copy last Christmas as part of their gifts as well. I have my copy of the book and it has helped me to understand others as well as myself more profoundly. Everyone one should get a copy for themself…a must have to help build better relationships.
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on December 31, 2023
★★★★★ 5
Prescriptive approach to eye-opening information
This book has changed my perspective for the better. It is very prescriptive in its approach, but that's just what I needed. Not only can I better understand the love expressed by others in their unique way, but I have a better concept of how to express love in different "languages" and even "dialects." My understanding of others has greatly improved. I'm more excited to think of different ways to express my love. I just can't say enough about this book. It is well worth a second read as well just to be reminded of all the important messages. It also includes portions about controlling anger and apologies.
The book doesn't contain information that's mind blowing, but the way it's presented can be life changing for some. It's the package deal on how to be a better lover.
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on March 12, 2018
★★★★★ 5
Recommend this book
Gary Chapman defines the daily tasks of Love and the work made to sustain a relationship as compared to the false notions that the media portrays about love which are actually false.
This is new learning and excellent book on love.
I only recommend this book on love and no other.
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on December 22, 2023
recommand products
Mad Professor Meets Jah9: Mad Professor Meets Jah9 In The Midst Of The Storm - VINYL LP
26.98
Protomartyr: Relatives In Descent - VINYL LP
26.99
Evil Invaders: Feed Me Violence - VINYL LP
19.98
ZZ Top: ZZ Top's First Album - VINYL LP
26.09
Bosaina: New York April-july 2013 / Two Names Upon Shore - VINYL LP
25.98