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Description
Human KRT17 ELISA KitProduct Specification Usage Experimental equipment required for the experiment: 1. Microplate reader (450nm) 2. High precision pipette and gun tips: 0. 5 10uL, 5 50uL, 20 200uL, 200 1000uL 3. 37 constant temperature box 4. Distilled water or deionized water Sample processing and requirements: Serum: Place the whole blood sample collected in the serum separation tube at room temperature for 2 hours or at 4 overnight, then centrifuge at 1000g for 20
Product Specification
| Usage |
Experimental equipment required for the experiment: 1. Microplate reader (450nm) 2. High-precision pipette and gun tips: 0.5-10uL, 5-50uL, 20-200uL, 200-1000uL 3. 37℃ constant temperature box 4. Distilled water or deionized water Sample processing and requirements: Serum: Place the whole blood sample collected in the serum separation tube at room temperature for 2 hours or at 4℃ overnight, then centrifuge at 1000×g for 20 minutes, and take the supernatant, or store the supernatant at -20℃ or -80℃, but avoid repeated freezing and thawing. Plasma: Collect the specimen using EDTA or heparin as an anticoagulant. Centrifuge the specimen at 1000 × g for 15 minutes at 2-8°C within 30 minutes of collection. The supernatant can be assayed or stored at -20°C or -80°C, but avoid repeated freezing and thawing. Tissue homogenization: Rinse the tissue with pre-chilled PBS (0.01M, pH 7.4) to remove residual blood (lysed red blood cells in the homogenate will affect the measurement results). Weigh the tissue and mince it. Add the minced tissue to the appropriate volume of PBS (generally a 1:9 weight-to-volume ratio, e.g., 1 g of tissue sample to 9 mL of PBS. The specific volume can be adjusted according to experimental needs and recorded. It is recommended to add protease inhibitors to the PBS) in a glass homogenizer and grind thoroughly on ice. To further lyse tissue cells, the homogenate can be sonicated or repeatedly frozen and thawed. Finally, centrifuge the homogenate at 5000 × g for 5-10 minutes, and the supernatant can be assayed. Cell Lysis Buffer: Gently wash adherent cells with ice-cold PBS, then trypsinize and collect cells by centrifugation at 1000×g for 5 minutes. Suspension cells can be collected directly by centrifugation. Wash collected cells three times with ice-cold PBS and resuspend in 150-200 μL of PBS per 1×10^6 cells (it is recommended to add protease inhibitors to the PBS; if the cell count is very low, reduce the PBS volume appropriately). Disrupt the cells by repeated freeze-thaw cycles or sonication. Centrifuge the extract at 1500×g for 10 minutes at 2-8°C, and remove the supernatant for analysis. Cell Culture Supernatant: Centrifuge at 1000×g for 20 minutes. Remove the supernatant for analysis or store at -20°C or -80°C, avoiding repeated freeze-thaw cycles. Other biological fluids: Centrifuge at 1000xg for 20 minutes, remove the supernatant, and test. Pre-test preparation: 1. Remove the test kit from the refrigerator 10 minutes in advance and equilibrate to room temperature. 2. Prepare the standard gradient working solution: Add 1 mL of universal diluent to the lyophilized standard, let it stand for 15 minutes to completely dissolve, then gently mix (concentration is 10 ng/mL). Then dilute to the following concentrations: 10 ng/mL, 5 ng/mL, 2.5 ng/mL, 1.25 ng/mL, 0.625 ng/mL, 0.3125 ng/mL, 0.15625 ng/mL, and 0 ng/mL. Serial dilution method: Take seven EP tubes and add 500uL of universal diluent to each. Pipette 500uL of the 10ng/mL standard working solution into the first EP tube and mix thoroughly to make a 5ng/mL standard working solution. Repeat this procedure for subsequent tubes. The last tube serves as a blank well; there is no need to pipette liquid from the penultimate tube. See the figure below for details. 3. Preparation of biotinylated detection antibody working solution: Centrifuge the concentrated biotinylated antibody at 1000×g for 1 minute 15 minutes before use. Dilute the 100× concentrated biotinylated antibody to a 1× working concentration with universal diluent (e.g., 10uL concentrate + 990uL universal diluent). Prepare and use immediately. 4. Prepare the enzyme conjugate working solution: 15 minutes before use, centrifuge the 100× concentrated enzyme conjugate at 1000×g for 1 minute. Dilute the 100× concentrated HRP enzyme conjugate to a 1× working concentration with universal diluent (e.g., 10 μL of concentrate + 990 μL of universal diluent). Prepare immediately. 5. Prepare the 1× wash solution: Dispense 10 mL of 20× wash solution into 190 mL of distilled water (concentrated wash solution removed from the refrigerator may crystallize; this is normal. Allow to stand at room temperature until the crystals have completely dissolved before preparing). Procedure: 1. Remove the desired strips from the aluminum foil bag after equilibration at room temperature for 10 minutes. Seal the remaining strips in a ziplock bag and return to 4°C. 2. Sample addition: Add 100 μL of sample or standard of varying concentrations to the corresponding wells. Add 100 μL of universal diluent to the blank wells. Cover with a film and incubate at 37°C for 60 minutes. (Recommendation: Dilute the sample to be tested at least 1-fold with universal diluent before adding it to the ELISA plate. This will reduce the impact of matrix effects on the test results. The sample concentration should be multiplied by the corresponding dilution factor when calculating the final sample concentration. It is recommended to run replicates for all test samples and standards.) 3. Add Biotinylated Antibody: Remove the ELISA plate and discard the liquid without washing. Add 100 μL of Biotinylated Antibody Working Solution directly to each well. Cover with a film and incubate at 37°C for 60 minutes. 4. Wash: Discard the liquid and add 300 μL of 1x Wash Solution to each well. Let stand for 1 minute, shake off the wash solution, and pat dry on absorbent paper. Repeat this process three times (a plate washer can also be used). 5. Add Enzyme Conjugate Working Solution: Add 100 μL of Enzyme Conjugate Working Solution to each well. Cover with a film and incubate at 37°C for 30 minutes. 6. Washing: Discard the liquid and wash the plate five times as in step 4. 7. Adding substrate: Add 90 μL of substrate (TMB) to each well, cover with a sealing film, and incubate at 37°C in the dark for 15 minutes. 8. Adding stop solution: Remove the ELISA plate and add 50 μL of stop solution directly to each well. Immediately measure the OD value of each well at a wavelength of 450 nm. Calculating experimental results: 1. Calculate the average OD value of the standard and sample replicates and subtract the OD value of the blank well as a correction factor. Plot the standard curve of the four-parameter logistic function on double-logarithmic graph paper, with concentration as the horizontal axis and OD value as the vertical axis. 2. If the sample OD value is higher than the upper limit of the standard curve, dilute the sample appropriately and retest. Multiply the sample concentration by the corresponding dilution factor. |
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| Theory | This kit utilizes a double-antibody sandwich enzyme-linked immunosorbent assay (ELISA). Sample, standard, biotin-labeled detection antibody, and HRP conjugate are sequentially added to microwells pre-coated with a keratin 17 (KRT17) capture antibody. After incubation and washing, the sample is developed using the substrate TMB. TMB is converted to blue by peroxidase (HRP) catalysis and to yellow by acid. The intensity of the color is positively correlated with the amount of keratin 17 (KRT17) in the sample. The absorbance (OD) is measured at 450 nm using a microplate reader to calculate the sample concentration. | |||||||||||||||||||||||||||||||||
| Source | Human | |||||||||||||||||||||||||||||||||
| Synonym | Human Keratin 17 ELISA Kit | |||||||||||||||||||||||||||||||||
| Detection Type | Double antibody sandwich method | |||||||||||||||||||||||||||||||||
| Composition |
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| Background | Keratin 17 (KRT17) is encoded by the KRT17 gene. Keratin 17 is a type I cytokeratin found in the nail bed, hair follicles, sebaceous glands, and other epidermal appendages. Mutations in the gene encoding this protein cause PC-K17 (formerly known as Jackson-Lawler) pachyderma congenita and multiple steatocysts. Keratin 17 (K17), a "psoriasis-associated cytokeratin," shares the same sequence as Streptococcal M protein (ALEEAN). It specifically stimulates psoriatic T cells, leading to their activation and release of inflammatory cytokines such as IFN-γ. IFN-γ, in turn, upregulates K17 expression through the STAT1 signaling pathway, triggering an autoimmune response against K17. This creates a vicious cycle that leads to pathological changes such as inflammation and abnormal KC proliferation, becoming a key component of the pathogenesis of psoriasis. Due to its high expression in psoriatic lesions and its role in stimulating T cell activation, K17 has been identified as a candidate target antigen recognized by autoreactive T cells in psoriasis. | |||||||||||||||||||||||||||||||||
| General Notes | 1. Strictly adhere to the specified incubation time and temperature to ensure accurate results. All reagents must be at room temperature (20-25°C) before use. Refrigerate reagents immediately after use. 2. Improper plate washing may result in inaccurate results. Ensure that all liquid in the wells is aspirated thoroughly before adding substrate. Do not allow the wells to dry out during incubation. 3. Remove any residual liquid and fingerprints from the bottom of the plate, as this will affect the OD value. 4. The substrate developer solution should be colorless or very light in color. Do not use substrate solution that has turned blue. 5. Avoid cross-contamination of reagents and specimens to prevent erroneous results. 6. Avoid direct exposure to strong light during storage and incubation. 7. Do not expose any reagents to bleaching solvents or the strong fumes emitted by bleaching solvents. Any bleaching agent will destroy the biological activity of the reagents in the kit. 8. Do not use expired products, and do not mix components with different product numbers and batches. 9. Recombinant proteins from sources other than the kit may not be compatible with the antibodies in this kit and will not be recognized. 10. If there is a possibility of disease transmission, all samples should be managed properly and samples and testing devices should be handled according to prescribed procedures. |
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| Storage Temp. | If the unopened kit is stored at 4°C, the shelf life is 6 months. | |||||||||||||||||||||||||||||||||
| Test Range | 0.15-10 ng/mL | |||||||||||||||||||||||||||||||||
| Applications | Serum, plasma, tissue homogenate, cell lysate, cell culture supernatant and other biological fluids |
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4.4 ★★★★★
Based on 20 reviews
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Product Reviews
★★★★★ 5
Great socks!
Size: Large, Color: (001) Black / Black / White
These fit true to size. Good socks.
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on February 23, 2026
★★★★★ 5
Great for walking all day at work as well
Size: X-Large, Color: (001) Black / Black / White
I walk approximately 10 miles a day at work, in a manufacturing setting. On concrete with steel toe shoes. My feet have been begging me to change from the socks that I have worn for years. I have worn these other name brand socks for probably 10 years. So, change was obviously something I had to be pushed into. I got on Amazon and searched for training socks. I read the description. Glanced at the reviews. I decided to move forward with purchasing these. I could not have been happier. My feet began feeling better immediately. And the priced was very reasonible.
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on April 12, 2026
★★★★★ 5
You have one pair of feet. Treat them kindly with a pair of UA socks
Size: Large, Color: (100) White / White / Black
Soft and tough. Easy on the feet. Nice fit prevents sore spots and blisters.
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Reviewed in the United States on December 3, 2025
★★★★★ 5
Excellent all-poly athletic socks for general lifting and HIIT.
Size: Medium, Color: Black/White/Grey
***These are NOT “low” -cut socks as the description in the title states. These are actually “quarter”-cut socks as clearly visible in the picture of the sock itself. “Low” -cut socks are shorter than this height but still visible with a shoe. Also, on the actual UA package of these socks, it clearly states these are QUARTER cut socks.
My review: Gave these UA Performance Tech socks (UAPT) 5 stars since they’re very good but in all reality not the best socks I’ve ever had for lifting and HIIT workouts. That title goes to Nike Dri-Fit MAX socks (DFM)….but these socks are really good. Like Nike’s DFM’s, these UAPT socks come in sizes, halleluah (M, L, and XL). The idea that most athletic sock are sized 6-12 and should therefore fit everyone perfectly is absolutely ridiculous. Your shoes come in whole and half sizes…so socks should at the very least be sized like these UAPT’s and Nike DFM’s…in S/M/L or in this case, M/L/XL. I wear a men’s size 8 shoe and the M (medium) size in these socks was a perfect fit; true to size.
Also, UAPT socks are ALL polyester with Spandex which is ideal and 100% that which you want for athletic socks: wicking with stretch. Cotton socks are the absolute worst thing you could wear to the gym since they’re sponges for sweat and therefore get wet and heavy, cause blisters, tend to stink, stick to skin, etc… IF you prefer cotton socks for athletics, then you don’t understand how performance fabrics work in athletics and training. You do NOT want cotton in your athletic apparel. You want your fabrics that wick sweat away; NOT soak up sweat and hold onto it. Also, the flexibility and stretch of the foot and ankle parts of the sock were both just right. Snug but not too tight. Wicking is perfect, too. Already worn during two 3 1/2 hour workouts and my feet stayed as dry as a desert.
When looking for UA athletic socks on Amazon, I surprisingly continued to find UA “athletic” socks made with mostly cotton which completely surprised me because a serious athletic company should produce an abundance of performance-level socks… i.e. all-poly socks. Thank goodness I finally came across these UA all-poly Performance Techs. The only drawback to these UAPT’s…and it’s a minor drawback…is that unlike Nike DFM’s, these do not come in L & R (left and rights) to specifically match the individual foot shape. Nike DFM socks come in L and R which sets them apart from all other athletic socks…and yet I don’t like supporting a massive monopolistic athletic apparel company like Nike, but they’re the only one that makes THE best athletic sock.
Nonetheless, these UAPT’s are perfect workout/HIIT/lifting socks for the bundle value price of six pair and three colors. If you’re looking to match your new UA or Rock shoes with the appropriate UA athletic sock, then here you go. I chose the black/white/gray mix bundle since I have new pairs of a black and a white version of the same UA-Rock shoe. These UAPT socks are medium thickness so they didn’t take up an exorbitant amount of room in my shoes but they’re thick enough to prevent blisters and provide cushion. Overall they’re very comfortable, hold a good stretch, hold their shape, and seem to be durable but only time will tell.
And since we’re on the black and white sock subject, I couldn’t resist: I read a shoe review about someone angered that his new UA/Rock black shoes (obviously bought for casual wear in lieu of gym use) staining his white cotton socks. First of all, never ever wear white socks (much less cotton white socks) with black athletic shoes! The possibility of staining is always there and the appearance just doesn’t work. However, you can get away with black socks with white shoes in some cases…but never white socks with black shoes. Why? Who knows? It’s just one of those unwritten rules of the gym. And no, I didn’t make the rules…so don’t blame the messenger. LOL!
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Reviewed in the United States on July 28, 2025
★★★★★ 5
Very soft and wears well
Size: Medium, Color: Black/White/Grey
Very nice quality and soft
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on April 18, 2026