Pay in installments of $46.03 with
,
and
Shipping Estimate
USA
- USA
- CAN
- USA
- CAN
Ships within 48 hours · Estimated delivery Aug 11 - Aug 16
Promo Codes Available:
For Your Every Summer RSVP, with Code: SUMMER15
Description
Rat ANG-1 ELISA KitProduct Specification Usage Experimental equipment required for the experiment: 1. Microplate reader (450nm) 2. High precision pipette and gun tips: 0. 5 10uL, 5 50uL, 20 200uL, 200 1000uL 3. 37 constant temperature box 4. Distilled water or deionized water Sample processing and requirements: 1. Serum: Place the whole blood sample collected in the serum separation tube at room temperature for 2 hours or at 4 overnight, then centrifuge at 1000g for 20
Product Specification
| Usage |
Experimental equipment required for the experiment: 1. Microplate reader (450nm) 2. High-precision pipette and gun tips: 0.5-10uL, 5-50uL, 20-200uL, 200-1000uL 3. 37℃ constant temperature box 4. Distilled water or deionized water Sample processing and requirements: 1. Serum: Place the whole blood sample collected in the serum separation tube at room temperature for 2 hours or at 4℃ overnight, then centrifuge at 1000×g for 20 minutes, and take the supernatant, or store the supernatant at -20℃ or -80℃, but avoid repeated freezing and thawing. 2. Plasma: Collect the specimen using EDTA or heparin as an anticoagulant. Centrifuge the specimen at 1000 × g for 15 minutes at 2-8°C within 30 minutes of collection. The supernatant can be assayed or stored at -20°C or -80°C, but avoid repeated freezing and thawing. 3. Tissue homogenization: Rinse the tissue with pre-chilled PBS (0.01M, pH 7.4) to remove residual blood (lysed red blood cells in the homogenate will affect the measurement results). Weigh the tissue and mince it. Add the minced tissue to the appropriate volume of PBS (generally a 1:9 weight-to-volume ratio, e.g., 1 g of tissue sample to 9 mL of PBS. The specific volume can be adjusted according to experimental needs and recorded. It is recommended to add protease inhibitors to the PBS) in a glass homogenizer and grind thoroughly on ice. To further lyse tissue cells, the homogenate can be sonicated or repeatedly frozen and thawed. Finally, centrifuge the homogenate at 5000 × g for 5-10 minutes, and the supernatant can be assayed. 4. Other biological fluids: Centrifuge at 1000xg for 20 minutes, remove the supernatant, and test. Pre-test preparation: 1. Remove the test kit from the refrigerator 10 minutes in advance and equilibrate to room temperature. 2. Prepare the standard gradient working solution: Add 1 mL of universal diluent to the lyophilized standard, let it stand for 15 minutes to completely dissolve, then gently mix (concentration is 200 pg/mL). Then dilute to the following concentrations: 200 pg/mL, 100 pg/mL, 50 pg/mL, 25 pg/mL, 12.5 pg/mL, 6.25 pg/mL, 3.125 pg/mL, and 0 pg/mL. Serial dilution method: Take seven EP tubes and add 500uL of universal diluent to each. Pipette 500uL of the 200pg/mL standard working solution into the first EP tube and mix thoroughly to make a 100pg/mL standard working solution. Repeat this procedure for subsequent tubes. The last tube serves as a blank well; there is no need to pipette liquid from the penultimate tube. See the figure below for details. 3. Preparation of biotinylated detection antibody working solution: Centrifuge the concentrated biotinylated antibody at 1000×g for 1 minute 15 minutes before use. Dilute the 100× concentrated biotinylated antibody to a 1× working concentration with universal diluent (e.g., 10uL concentrate + 990uL universal diluent). Prepare and use immediately. 4. Prepare the enzyme conjugate working solution: 15 minutes before use, centrifuge the 100× concentrated enzyme conjugate at 1000×g for 1 minute. Dilute the 100× concentrated HRP enzyme conjugate to a 1× working concentration with universal diluent (e.g., 10 μL of concentrate + 990 μL of universal diluent). Prepare immediately. 5. Prepare the 1× wash solution: Dispense 10 mL of 20× wash solution into 190 mL of distilled water (concentrated wash solution removed from the refrigerator may crystallize; this is normal. Allow to stand at room temperature until the crystals have completely dissolved before preparing). Procedure: 1. Remove the desired strips from the aluminum foil bag after equilibration at room temperature for 10 minutes. Seal the remaining strips in a ziplock bag and return to 4°C. 2. Sample addition: Add 100 μL of sample or standard of varying concentrations to the corresponding wells. Add 100 μL of universal diluent to the blank wells. Cover with a film and incubate at 37°C for 60 minutes. (Recommendation: Dilute the sample to be tested at least 1-fold with universal diluent before adding it to the ELISA plate. This will reduce the impact of matrix effects on the test results. The sample concentration should be multiplied by the corresponding dilution factor when calculating the final sample concentration. It is recommended to run replicates for all test samples and standards.) 3. Add Biotinylated Antibody: Remove the ELISA plate and discard the liquid without washing. Add 100 μL of Biotinylated Antibody Working Solution directly to each well. Cover with a film and incubate at 37°C for 60 minutes. 4. Wash: Discard the liquid and add 300 μL of 1x Wash Solution to each well. Let stand for 1 minute, shake off the wash solution, and pat dry on absorbent paper. Repeat this process three times (a plate washer can also be used). 5. Add Enzyme Conjugate Working Solution: Add 100 μL of Enzyme Conjugate Working Solution to each well. Cover with a film and incubate at 37°C for 30 minutes. 6. Washing: Discard the liquid and wash the plate five times as in step 4. 7. Adding substrate: Add 90 μL of substrate (TMB) to each well, cover with a sealing film, and incubate at 37°C in the dark for 15 minutes. 8. Adding stop solution: Remove the ELISA plate and add 50 μL of stop solution directly to each well. Immediately measure the OD value of each well at a wavelength of 450 nm. Calculating experimental results: 1. Calculate the average OD value of the standard and sample replicates and subtract the OD value of the blank well as a correction factor. Plot the standard curve of the four-parameter logistic function on double-logarithmic graph paper, with concentration as the horizontal axis and OD value as the vertical axis. 2. If the sample OD value is higher than the upper limit of the standard curve, dilute the sample appropriately and retest. Multiply the sample concentration by the corresponding dilution factor. |
|||||||||||||||||||||||||||||||||
| Theory | This kit utilizes a double-antibody sandwich enzyme-linked immunosorbent assay (ELISA). Sample, standard, biotin-labeled detection antibody, and HRP conjugate are sequentially added to microwells pre-coated with angiopoietin 1 (ANG-1) capture antibody. After incubation and washing, the sample is developed using the substrate TMB. TMB is converted to blue by HRP peroxidase and to yellow by acid. The intensity of the color is positively correlated with the amount of angiopoietin 1 (ANG-1) in the sample. The absorbance (OD) is measured at 450 nm using a microplate reader to calculate the sample concentration. | |||||||||||||||||||||||||||||||||
| Source | Rat | |||||||||||||||||||||||||||||||||
| Synonym | Rat Angiopoietin 1 ELISA Kit | |||||||||||||||||||||||||||||||||
| Detection Type | Double antibody sandwich method | |||||||||||||||||||||||||||||||||
| Composition |
|
|||||||||||||||||||||||||||||||||
| Background | Angiopoietin 1 is a type of angiopoietin, encoded by the gene ANGPT1. Angiopoietins are proteins that play an important role in vascular development and angiogenesis. All angiopoietins bind with similar affinity to endothelial cell-specific tyrosine protein kinase receptors. The protein encoded by this gene is a secreted glycoprotein that activates the receptor by inducing tyrosine phosphorylation. It plays a key role in mediating interactions between endothelial cells and the surrounding matrix and interstitial tissue. This protein also contributes to vascular maturation and stability and may be involved in early cardiac development. | |||||||||||||||||||||||||||||||||
| General Notes | 1. Strictly adhere to the specified incubation time and temperature to ensure accurate results. All reagents must be at room temperature (20-25°C) before use. Refrigerate reagents immediately after use. 2. Improper plate washing may result in inaccurate results. Ensure that all liquid in the wells is aspirated thoroughly before adding substrate. Do not allow the wells to dry out during incubation. 3. Remove any residual liquid and fingerprints from the bottom of the plate, as this will affect the OD value. 4. The substrate developer solution should be colorless or very light in color. Do not use substrate solution that has turned blue. 5. Avoid cross-contamination of reagents and specimens to prevent erroneous results. 6. Avoid direct exposure to strong light during storage and incubation. 7. Do not expose any reagents to bleaching solvents or the strong fumes emitted by bleaching solvents. Any bleaching agent will destroy the biological activity of the reagents in the kit. 8. Do not use expired products, and do not mix components with different product numbers and batches. 9. Recombinant proteins from sources other than the kit may not be compatible with the antibodies in this kit and will not be recognized. 10. If there is a possibility of disease transmission, all samples should be managed properly and samples and testing devices should be handled according to prescribed procedures. |
|||||||||||||||||||||||||||||||||
| Storage Temp. | If the unopened kit is stored at 4°C, the shelf life is 6 months. | |||||||||||||||||||||||||||||||||
| Test Range | 3.12-200 pg/mL | |||||||||||||||||||||||||||||||||
| Applications | Serum, plasma, tissue homogenates and other biological fluids |
Shipping Notes
- Free Standard Shipping on $100+ Orders to the USA.
- Except Preorder products are shipped in 48 hours.
- Delivery to the USA:
- Standard Shipping : 3-10 business days
- If time is of the essence, please consider selecting expedited delivery for faster service.
Exchange/Return Notes
- We offer a 30-day return/exchange service after receiving.
- Final sale items are not eligible for returns or exchanges.
- To process your return/exchange, please contact us at [email protected]
- Please click here for more details>>> Return & Exchange Policy
4.2 ★★★★★
Based on 29 reviews
Sort
Product Reviews
★★★★★ 5
many feelings
Format: Kindle
I didn’t know what this book was about when I first started reading.. one thing I felt at the beginning was…amazement? I love writing letters, something I don’t do often, I recently bought this beautiful fountain pen, I wanted to start small, writing greeting cards to family and friends, birthday cards, thank yous, etc. what I didn’t expect is the emotional toll this book had on me, and other members of my family who read the book for our book club. The subject matters, death of a child, husband dying of cancer, the significance of May 29th, all impacted our family, and so, as I read this book, I had to put it down several times because I had to cry, compose myself, think of regrets I have, and remember.
How amazing it is that Sybil was able to write all about the death of her child to her dead child. I always say that writing it down on paper releases your burden in your head…it is true, I think writing letters, even the ones she didn’t send helped her quiet her head, at least for a while. I truly love this book. I have been borrowing books from Libby to read for our book club for a very long time, there have been books that I fell in love with, and always thought if I want to read them again I’ll just borrow them again, this book is the first one that I want to own physically to put on my shelf and know that I have it to read over and over again when I want to reflect and then quiet my head. ( This is the longest review I’ve written about a book I’ve read, I hope it makes sense).
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on May 21, 2026
★★★★★ 5
Successful Epistolary Novel
Format: Kindle
When we first meet Sybil Van Antwerp, age 73, she is retired from law and spends a lot of time writing letters and emails. She also reads a lot and is interested in what her correspondents are reading. She has children and grandchildren, but she lives alone in Annapolis, Maryland, and doesn’t see her family very often. During the course of reading her correspondence, we very gradually learn about Sybil—her history and her present troubles. She is a complicated character and several times in reading The Correspondent I paused to ask myself what I thought of Sybil—did I like her? What about her kept me from the immediate response a reader usually has about the main character of a book? Even at the end of the book, I am still ambivalent about Sybil, but I certainly understand her much better.
Sybil, herself, and the book have so many layers. There is true depth to the story. My book club took deep dives into it over a period of three weeks, and I don’t think we have sampled all the topics represented in this book. It is a wonderful novel for stimulating meaningful discussion.
Neurodivergence is not called out or named, but it comes to mind in thinking about Harry, a child who is the son of a judge, a former colleague of Sybil. The boy doesn’t quite fit in socially with his peers, but he is brilliant. Sybil makes the perfect “pen pal” for Harry because they have some of the same characteristics. As a child she was punished for “insolence and rudeness,” but her parents were just trying to mold her into a polite young lady as expected by society. She was blunt and didn’t have many friends.
There are so many other issues worthy of discussion, but they would most certainly bring up spoilers. I won’t do that to you. Readers should have the opportunity to see the story gradually emerge from the letters, including a continuing one that the reader doesn’t know to whom Sybil is writing. Sybil sets the word “stone” for secrets, and there are stones in this book making it a puzzle, a mystery of sorts—for the reader.
As a reviewer, I tend to go quickly from one book to the next as soon as I have composed and published my thoughts. Characters in various books can even blend together. This is not the case with The Correspondent. The characters in this book, especially Sybil, have stayed with me and come to mind frequently as I go through my day. Virginia Evans has created a fictional world with impact. Just as Sybil needed time (years in her case) to process the events of her life, the reader will need time to process them and their effects on Sybil as well.
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on March 15, 2026
★★★★★ 5
Better Than the Movie
Format: Hardcover, Format: Hardcover
I picked up Remarkably Bright Creatures after hearing so much buzz about it, and it absolutely lived up to the hype. This is one of those rare books that is heartfelt, funny, emotional, and completely unique all at the same time. The characters feel real and layered, and the story unfolds in such a thoughtful, satisfying way.
Marcellus the octopus completely steals the show. I didn’t expect to become so attached to an octopus, but his chapters were easily my favorite part of the book. They added humor, wisdom, and so much heart to the story.
If you’ve already seen the movie adaptation but haven’t read the book yet, you absolutely should. The book goes so much deeper emotionally and gives so much more background and connection between the characters. I enjoyed the movie, but the book made me care about the story on a completely different level.
The writing is approachable and easy to get into, but it still leaves a lasting impact. This would make a great book club pick or gift for anyone who enjoys character-driven stories with warmth and emotional depth.
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on May 25, 2026
★★★★★ 5
Beautiful!
Format: Kindle
I heard about this book in a conversation at a craft party and ordered it the same night. I was not disappointed. I fell in love with Marcellus and Tova and their delightful relationship, and with all the ancillary characters that made this book so special. The ending was so very sweet and satisfying as well. Great 1st book!
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on May 27, 2026
★★★★★ 4
A Remarkable Book--I Want More Like It!
Format: Kindle
I stumbled on Remarkably Bright Creatures among my recommendations. As often happens, the premise intrigued me enough to read it even though I didn't know the author. Well, if Shelby Van Pelt often writes like this, I definitely want more of her books.
The friendship between Tova the aquarium cleaning lady and Marcellus the giant Pacific octopus is a definite selling point in this book. It's a great twist on the "A Kid and Their X" trope (the kind of thing you see in stuff like Where the Red Fern Grows, Old Yeller, Free Willy, and so forth). This one, I am pleased to report, is far less cheesy and saccharine. Tova is a multifaceted character whose life may seem small in her estimation, and probably is if we go by what is a "normal" life in a small seaside town. Yet that life has been stuffed with complex emotions, unanswered questions, and a longing for "more" that I think everybody can relate to. At least, I certainly could.
As for Marcellus, he's multifaceted, too. A part of me did stick with his point of view, and story, because it is a story of a "remarkably bright," underestimated creature trapped and controlled in a world he doesn't understand. I have cerebral palsy and was diagnosed as level 1 autistic this spring; I'm still looking back and trying to heal from a lot of the latter's implications. So in a visceral way, yeah. I'm not a captive animal or mistreated, but I understood how Marcellus felt. What I liked even better about his story though, is that Marcellus' story doesn't stop there.
Marcellus actually has a life and a history that Shelby Van Pelt, Tova, Cameron, and other characters dig into. He's not just a captive yearning for freedom and mourning lost opportunities, as often happens with similar stories. He makes wry, witty, yet quiet, non-snarky observations about humans. He cares deeply for Tova because she took time to know him as a "person," but also because she's the only human who has ever stopped long enough for Marcellus to know as a real "person." Marcellus gets to be the conduit of real revelation and change for people inside and outside his world. That's amazing, and frankly, it's a lot more than many human characters get.
Remarkably Bright Creatures couldn't succeed with just Tova or Marcellus though, or at least not as well as it did, I think. The other characters pop off the page as well. Cameron is my favorite, precisely because I didn't expect to like him at first. By his own admission, he comes off as a burnout and a loser, and I admit, that's how I saw him to begin with. But as with Tova and Marcellus, Cameron is hiding a lot of layers, a lot of depth. He's got a great heart, but he's still a typical, kinda clueless 30-year-old guy (and I'm still a 30-something so I say that tongue in cheek). He's vulnerable but not pitiful; he plays the victim sometimes, but then he gets mad at being called out on that and wants to do better. He struggles and is a burnout, but he's got good, solid friends and some real interests and potential. In other words, I got really into his arc, especially once Tova, Marcellus, and even Ethan got involved.
As for Ethan, he's unexpectedly cool, as well. I admit some brownie points with him, as I love all things Scottish or Irish and he is of Scottish descent. But I also didn't expect a shopkeeper in his '70s with a gruff edge to be a Grateful Dead fan or to get along so well with a neat and tidy, tiny Swedish aquarium cleaner, so brownie points on plot and character there. In fact, Ethan's role in the story, in particular, went all kinds of unexpected places, so without spoiling anything, I'll tell you to keep an eye on it.
Finally, Shelby wins some extra brownie points for a lot of the "little" things that "pop" out at me when reading a book like this one. For instance, I love cats and love the reluctant yet affectionate relationship Tova develops with a stray cat. I love Marcellus' Collection and the pride and care he takes in it. I love and felt some heartbreak over Tova's reminiscing about the attic playroom that never got to be a playroom.
But I think the thing I loved most about Remarkably Bright Creatures is how remarkable Shelby showed readers humans--and creatures--can be, without getting heavy-handed or cheesy. There are some romances here, so to speak, but this is a relationship novel, and those are, have been for the past several years, some of my favorite ones.
There is some language here, so caveat emptor if that's not your thing. From a writer's perspective, I also wondered how much some threads or characters were actually needed. For instance, looking back, I understand why Tova had a brother, but I'm unsure how much he furthered the plot. Similarly, I understand the roles of Avery and Marco but again, am unsure how much they furthered the plot.
Also, I almost hate to say it, and I could be wrong about this. After all, I know about as much concerning octopi as the average person who watches Jeopardy (which I do). But while Marcellus' plot thread is great, and while I definitely understand suspension of disbelief, I did wonder along with Cameron how much those boundaries were pushed. I mean, I buy Marcellus getting out of his tank, and with the right Collection items, I guess Tova and Cameron could've figured out whatever they needed to know. But...there's a logical shard of me that isn't quite sure she buys how everything lined up, I guess.
All that aside though, Remarkably Bright Creatures is still a remarkable book. As often happens, I'm now eager to find more like it. And yes, it does get a hearty recommendation. At the very least, I'll want to take closer looks next time I go to an aquarium.
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on January 22, 2025