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Description
Rat MSLN ELISA KitProduct Specification Usage Experimental equipment required for the experiment: 1. Microplate reader (450nm) 2. High precision pipette and gun tips: 0. 5 10uL, 5 50uL, 20 200uL, 200 1000uL 3. 37 constant temperature box 4. Distilled water or deionized water Sample processing and requirements: Serum: Place the whole blood sample collected in the serum separation tube at room temperature for 2 hours or at 4 overnight, then centrifuge at 1000g for 20
Product Specification
| Usage |
Experimental equipment required for the experiment: 1. Microplate reader (450nm) 2. High-precision pipette and gun tips: 0.5-10uL, 5-50uL, 20-200uL, 200-1000uL 3. 37℃ constant temperature box 4. Distilled water or deionized water Sample processing and requirements: Serum: Place the whole blood sample collected in the serum separation tube at room temperature for 2 hours or at 4℃ overnight, then centrifuge at 1000×g for 20 minutes, and take the supernatant, or store the supernatant at -20℃ or -80℃, but avoid repeated freezing and thawing. Plasma: Collect the specimen using EDTA or heparin as an anticoagulant. Centrifuge the specimen at 1000 × g for 15 minutes at 2-8°C within 30 minutes of collection. The supernatant can be assayed or stored at -20°C or -80°C, but avoid repeated freezing and thawing. Tissue homogenization: Rinse the tissue with pre-chilled PBS (0.01M, pH 7.4) to remove residual blood (lysed red blood cells in the homogenate will affect the measurement results). Weigh the tissue and mince it. Add the minced tissue to the appropriate volume of PBS (generally a 1:9 weight-to-volume ratio, e.g., 1 g of tissue sample to 9 mL of PBS. The specific volume can be adjusted according to experimental needs and recorded. It is recommended to add protease inhibitors to the PBS) in a glass homogenizer and grind thoroughly on ice. To further lyse tissue cells, the homogenate can be sonicated or repeatedly frozen and thawed. Finally, centrifuge the homogenate at 5000 × g for 5-10 minutes, and the supernatant can be assayed. Other biological fluids: Centrifuge at 1000xg for 20 minutes, remove the supernatant, and test. Pre-test preparation: 1. Remove the test kit from the refrigerator 10 minutes in advance and equilibrate to room temperature. 2. Prepare the standard gradient working solution: Add 1 mL of universal diluent to the lyophilized standard, let it stand for 15 minutes to completely dissolve, then gently mix (concentration is 10 ng/mL). Then dilute to the following concentrations: 10 ng/mL, 5 ng/mL, 2.5 ng/mL, 1.25 ng/mL, 0.625 ng/mL, 0.3125 ng/mL, 0.15625 ng/mL, and 0 ng/mL. Serial dilution method: Take seven EP tubes and add 500uL of universal diluent to each. Pipette 500uL of the 10ng/mL standard working solution into the first EP tube and mix thoroughly to make a 5ng/mL standard working solution. Repeat this procedure for subsequent tubes. The last tube serves as a blank well; there is no need to pipette liquid from the penultimate tube. See the figure below for details. 3. Preparation of biotinylated detection antibody working solution: Centrifuge the concentrated biotinylated antibody at 1000×g for 1 minute 15 minutes before use. Dilute the 100× concentrated biotinylated antibody to a 1× working concentration with universal diluent (e.g., 10uL concentrate + 990uL universal diluent). Prepare and use immediately. 4. Prepare the enzyme conjugate working solution: 15 minutes before use, centrifuge the 100× concentrated enzyme conjugate at 1000×g for 1 minute. Dilute the 100× concentrated HRP enzyme conjugate to a 1× working concentration with universal diluent (e.g., 10 μL of concentrate + 990 μL of universal diluent). Prepare immediately. 5. Prepare the 1× wash solution: Dispense 10 mL of 20× wash solution into 190 mL of distilled water (concentrated wash solution removed from the refrigerator may crystallize; this is normal. Allow to stand at room temperature until the crystals have completely dissolved before preparing). Procedure: 1. Remove the desired strips from the aluminum foil bag after equilibration at room temperature for 10 minutes. Seal the remaining strips in a ziplock bag and return to 4°C. 2. Sample addition: Add 100 μL of sample or standard of varying concentrations to the corresponding wells. Add 100 μL of universal diluent to the blank wells. Cover with a film and incubate at 37°C for 60 minutes. (Recommendation: Dilute the sample to be tested at least 1-fold with universal diluent before adding it to the ELISA plate. This will reduce the impact of matrix effects on the test results. The sample concentration should be multiplied by the corresponding dilution factor when calculating the final sample concentration. It is recommended to run replicates for all test samples and standards.) 3. Add Biotinylated Antibody: Remove the ELISA plate and discard the liquid without washing. Add 100 μL of Biotinylated Antibody Working Solution directly to each well. Cover with a film and incubate at 37°C for 60 minutes. 4. Wash: Discard the liquid and add 300 μL of 1x Wash Solution to each well. Let stand for 1 minute, shake off the wash solution, and pat dry on absorbent paper. Repeat this process three times (a plate washer can also be used). 5. Add Enzyme Conjugate Working Solution: Add 100 μL of Enzyme Conjugate Working Solution to each well. Cover with a film and incubate at 37°C for 30 minutes. 6. Washing: Discard the liquid and wash the plate five times as in step 4. 7. Adding substrate: Add 90 μL of substrate (TMB) to each well, cover with a sealing film, and incubate at 37°C in the dark for 15 minutes. 8. Adding stop solution: Remove the ELISA plate and add 50 μL of stop solution directly to each well. Immediately measure the OD value of each well at a wavelength of 450 nm. Calculating experimental results: 1. Calculate the average OD value of the standard and sample replicates and subtract the OD value of the blank well as a correction factor. Plot the standard curve of the four-parameter logistic function on double-logarithmic graph paper, with concentration as the horizontal axis and OD value as the vertical axis. 2. If the sample OD value is higher than the upper limit of the standard curve, dilute the sample appropriately and retest. Multiply the sample concentration by the corresponding dilution factor. |
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| Theory | This kit utilizes a double-antibody sandwich enzyme-linked immunosorbent assay (ELISA). Sample, standard, biotin-labeled detection antibody, and HRP conjugate are sequentially added to microwells pre-coated with a mesothelin (MSLN) capture antibody. After incubation and washing, the sample is developed using the substrate TMB. TMB is converted to blue by peroxidase (HRP) catalysis and to yellow by acid. The intensity of the color is positively correlated with the amount of mesothelin (MSLN) in the sample. The absorbance (OD) is measured at 450 nm using a microplate reader to calculate the sample concentration. | |||||||||||||||||||||||||||||||||
| Source | Rat | |||||||||||||||||||||||||||||||||
| Synonym | Rat Mesothelin | |||||||||||||||||||||||||||||||||
| Detection Type | Double antibody sandwich method | |||||||||||||||||||||||||||||||||
| Composition |
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| Background | Mesothelin, also known as MSLN, is a protein encoded by the MSLN gene. It is a 40 kDa protein expressed in mesothelial cells. The protein was first identified by its reactivity with the monoclonal antibody K1. Subsequent cloning studies revealed that the gene encodes a precursor protein that is processed to produce mesothelin, which is attached to the cell membrane via a glycophosphatidylinositol bond and a 31 kDa shed fragment called megakaryocyte potentiating factor (MPF). Although it has been proposed that it may be involved in cell adhesion, its biological function remains unclear. Knockout rat strains lacking mesothelin reproduce and develop normally. It is overexpressed in several human tumors, including mesothelioma, ovarian cancer, pancreatic adenocarcinoma, lung adenocarcinoma, and bile duct carcinoma. It binds to MUC16 (also known as CA125), suggesting that its interaction with MUC16 may contribute to tumor implantation and peritoneal dissemination through cell adhesion. A 64-amino acid region (residues 296–359) at the N-terminus of cell surface endothelin has been identified as the functional binding domain of MUC16 (termed IAB), suggesting a mechanism by which it acts as a functional chaperone of MUC16 in cancer development. | |||||||||||||||||||||||||||||||||
| General Notes | 1. Strictly adhere to the specified incubation time and temperature to ensure accurate results. All reagents must be at room temperature (20-25°C) before use. Refrigerate reagents immediately after use. 2. Improper plate washing may result in inaccurate results. Ensure that all liquid in the wells is aspirated thoroughly before adding substrate. Do not allow the wells to dry out during incubation. 3. Remove any residual liquid and fingerprints from the bottom of the plate, as this will affect the OD value. 4. The substrate developer solution should be colorless or very light in color. Do not use substrate solution that has turned blue. 5. Avoid cross-contamination of reagents and specimens to prevent erroneous results. 6. Avoid direct exposure to strong light during storage and incubation. 7. Do not expose any reagents to bleaching solvents or the strong fumes emitted by bleaching solvents. Any bleaching agent will destroy the biological activity of the reagents in the kit. 8. Do not use expired products, and do not mix components with different product numbers and batches. 9. Recombinant proteins from sources other than the kit may not be compatible with the antibodies in this kit and will not be recognized. 10. If there is a possibility of disease transmission, all samples should be managed properly and samples and testing devices should be handled according to prescribed procedures. |
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| Storage Temp. | If the unopened kit is stored at 4°C, the shelf life is 6 months. | |||||||||||||||||||||||||||||||||
| Test Range | 0.156-10 ng/mL | |||||||||||||||||||||||||||||||||
| Applications | Serum, plasma, tissue homogenates and other biological fluids |
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4.9 ★★★★★
Based on 30 reviews
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Product Reviews
★★★★★ 5
It really cools - Extremely soft - Not too heavy
Size: Queen (90" * 90"）, Color: Luxury Grey, Size: Queen (90" * 90"）, Color: Luxury Grey
This comforter arrived packaged very well from the manufacturer. It arrived sealed in plastic inside a box. The color and appearance of the comforter received looks like what was shown in the listing. This comforter is extremely soft. It feels very cool to the touch. It has a great cooling effect. It is also not too heavy. A lot of cooling bedding products like this feel heavy. While this does have some weight it is not too much. It is easy to move around. The comforter is easy to refresh and cool off again after extended use. I just uncovered myself, lifted and moved the comforter around a little, then covered back up. This made it feel cooler again. I am very happy with this comforter so far. I like the color and design. I really like feeling cool when falling asleep. This will be great when the temperatures start going up. I will update this review later if anything changes after washing a few more times and longer use. I think this will be a good comforter for most hot sleepers who like to cover up all year.
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on April 18, 2026
★★★★★ 5
Feels seriously amazing!
Size: Twin（90“ * 67”）, Color: Luxury Grey
This is my second cooling comforter from this brand and this one is just as amazing as the first. It’s silky soft and makes a significant temperature difference. It’s like magic; you don’t need to do anything special to cool it before bed. If you do want extra coolness, you can place it in front of your AC or turn on your fan for a little bit before bed and It absorbs the extra coolness but either way it lasts all night. I use it with the cooling pillow, and the hot summer nights are no match. It’s easy to wash, really silky, smooth, and actually cool against the skin. It also somehow has a weighted blanket-like feel without being heavy. I standby this blanket so much that this is my second one, highly recommend!
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Reviewed in the United States on May 5, 2026
★★★★★ 5
Silky soft pillow cases save hair from tangles
Pillows feel great and keep your head cool plus your hair doesnt get all tangled at night. I have juwt put them out on my bed and havent had a chance to wash them but they seem like theyre made from quality material that should hold up in the wash.
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Reviewed in the United States on May 31, 2021
★★★★★ 5
Super Soft
Size: Queen (2 Count), Color: Gray
So I’ve been trying these pillowcases out for a while. Let me tell you if you’re looking for a silky soft comfortable surface to lay your head on look no further. These silky, soft satin pillowcases fill that niche like nobody else. The material is very soft it looks very shiny and feels amazing next to my skin, which is all I look for. The quality is excellent. I’ve really enjoyed these pillowcases and the silver is wearing on me now I need to order some matching silver silky sheets, and I’ll be set. What an amazing value.
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Reviewed in the United States on November 4, 2025
★★★★★ 4
4 BUCKS PER PILLOWCASE. DEAL!
Size: Queen (2 Count), Color: Gray
SUPA MODERN Satin Pillowcase, Similar to Silk Pillow Cases Queen Size Set of 2.
These silver pillowcases shine like metal, but they are worth 4 bucks a piece. My sister and I each tried one out. She loves how kindly the pillowcase treats her hair and her extensions; no snags, knottting, or rat nests.
I love the way the material feels on my face; soothing and smooth. Definitely a good, affordable price for pillowcases, even shiny silver ones.
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Reviewed in the United States on November 13, 2025
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