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Description
Human EXOSC7 ELISA KitProduct Specification Usage Required experimental equipment: 1. Microplate reader (450nm) 2. High precision pipettes and pipette tips: 0. 5 10uL, 5 50uL, 20 200uL, 200 1000uL 3. 37C incubator 4. Distilled or deionized water Sample preparation and requirements: Tissue homogenization: Rinse the tissue with pre chilled PBS (0. 01M, pH 7. 4) to remove residual blood (lysed red blood cells in the homogenate will affect the measurement results). Weigh and
Product Specification
| Usage | Required experimental equipment: 1. Microplate reader (450nm) 2. High-precision pipettes and pipette tips: 0.5-10uL, 5-50uL, 20-200uL, 200-1000uL 3. 37°C incubator 4. Distilled or deionized water Sample preparation and requirements: Tissue homogenization: Rinse the tissue with pre-chilled PBS (0.01M, pH 7.4) to remove residual blood (lysed red blood cells in the homogenate will affect the measurement results). Weigh and mince the tissue. Add the minced tissue to the appropriate volume of PBS (generally a 1:9 weight-to-volume ratio, e.g., 1g of tissue sample to 9mL of PBS. The specific volume can be adjusted according to experimental needs and recorded. It is recommended to add protease inhibitors to the PBS) in a glass homogenizer and grind thoroughly on ice. To further lyse tissue cells, the homogenate can be sonicated or repeatedly frozen and thawed. Finally, centrifuge the homogenate at 5000×g for 5-10 minutes, and collect the supernatant for analysis. Cell Lysis Buffer: Adherent cells should be gently washed with pre-chilled PBS, then trypsinized and harvested by centrifugation at 1000×g for 5 minutes. Suspension cells can be harvested directly by centrifugation. Collected cells should be washed three times with pre-chilled PBS and resuspended in 150-200 μL of PBS per 1×10^6 cells (it is recommended to add protease inhibitors to the PBS; if the cell count is very low, reduce the PBS volume appropriately). Disrupt the cells by repeated freezing and thawing or sonication. Centrifuge the extract at 1500×g for 10 minutes at 2-8°C, and collect the supernatant for analysis. Other biological fluids: Centrifuge at 1000xg for 20 minutes, remove the supernatant, and test. Pre-test preparation: 1. Remove the test kit from the refrigerator 10 minutes in advance and equilibrate to room temperature. 2. Prepare the standard gradient working solution: Add 1 mL of universal diluent to the lyophilized standard, let it stand for 15 minutes to completely dissolve, then gently mix (concentration is 10 ng/mL). Then dilute to the following concentrations: 10 ng/mL, 5 ng/mL, 2.5 ng/mL, 1.25 ng/mL, 0.625 ng/mL, 0.3125 ng/mL, 0.15625 ng/mL, and 0 ng/mL. Serial dilution method: Take seven EP tubes and add 500uL of universal diluent to each. Pipette 500uL of the 10ng/mL standard working solution into the first EP tube and mix thoroughly to make a 5ng/mL standard working solution. Repeat this procedure for subsequent tubes. The last tube serves as a blank well; there is no need to pipette liquid from the penultimate tube. See the figure below for details. 3. Preparation of biotinylated detection antibody working solution: Centrifuge the concentrated biotinylated antibody at 1000×g for 1 minute 15 minutes before use. Dilute the 100× concentrated biotinylated antibody to a 1× working concentration with universal diluent (e.g., 10uL concentrate + 990uL universal diluent). Prepare and use immediately. 4. Prepare the enzyme conjugate working solution: 15 minutes before use, centrifuge the 100× concentrated enzyme conjugate at 1000×g for 1 minute. Dilute the 100× concentrated HRP enzyme conjugate to a 1× working concentration with universal diluent (e.g., 10 μL of concentrate + 990 μL of universal diluent). Prepare immediately. 5. Prepare the 1× wash solution: Dispense 10 mL of 20× wash solution into 190 mL of distilled water (concentrated wash solution removed from the refrigerator may crystallize; this is normal. Allow to stand at room temperature until the crystals have completely dissolved before preparing). Procedure: 1. Remove the desired strips from the aluminum foil bag after equilibration at room temperature for 10 minutes. Seal the remaining strips in a ziplock bag and return to 4°C. 2. Sample addition: Add 100 μL of sample or standard of varying concentrations to the corresponding wells. Add 100 μL of universal diluent to the blank wells. Cover with a film and incubate at 37°C for 60 minutes. (Recommendation: Dilute the sample to be tested at least 1-fold with universal diluent before adding it to the ELISA plate. This will reduce the impact of matrix effects on the test results. The sample concentration should be multiplied by the corresponding dilution factor when calculating the final sample concentration. It is recommended to run replicates for all test samples and standards.) 3. Add Biotinylated Antibody: Remove the ELISA plate and discard the liquid without washing. Add 100 μL of Biotinylated Antibody Working Solution directly to each well. Cover with a film and incubate at 37°C for 60 minutes. 4. Wash: Discard the liquid and add 300 μL of 1x Wash Solution to each well. Let stand for 1 minute, shake off the wash solution, and pat dry on absorbent paper. Repeat this process three times (a plate washer can also be used). 5. Add Enzyme Conjugate Working Solution: Add 100 μL of Enzyme Conjugate Working Solution to each well. Cover with a film and incubate at 37°C for 30 minutes. 6. Washing: Discard the liquid and wash the plate five times as in step 4. 7. Adding substrate: Add 90 μL of substrate (TMB) to each well, cover with a sealing film, and incubate at 37°C in the dark for 15 minutes. 8. Adding stop solution: Remove the ELISA plate and add 50 μL of stop solution directly to each well. Immediately measure the OD value of each well at a wavelength of 450 nm. Calculating experimental results: 1. Calculate the average OD value of the standard and sample replicates and subtract the OD value of the blank well as a correction factor. Plot the standard curve of the four-parameter logistic function on double-logarithmic graph paper, with concentration as the horizontal axis and OD value as the vertical axis. 2. If the sample OD value is higher than the upper limit of the standard curve, dilute the sample appropriately and retest. Multiply the sample concentration by the corresponding dilution factor. |
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| Theory | This kit uses a double-antibody sandwich enzyme-linked immunosorbent assay (ELISA). Sample, standard, biotin-labeled detection antibody, and HRP conjugate are sequentially added to microwells pre-coated with an Exosome Component 7 (EXOSC7) capture antibody. After incubation and washing, the assay is developed using the substrate TMB. TMB converts to blue under the catalysis of HRP and to yellow under the action of acid. The intensity of the color is positively correlated with the amount of Exosome Component 7 (EXOSC7) in the sample. The absorbance (OD) is measured at 450 nm using a microplate reader to calculate the sample concentration. | |||||||||||||||||||||||||||||||||
| Source | Human | |||||||||||||||||||||||||||||||||
| Synonym | Human Exosome Component 7 ELISA Kit | |||||||||||||||||||||||||||||||||
| Detection Type | Double antibody sandwich method | |||||||||||||||||||||||||||||||||
| Composition |
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| Background | Exosome component 7 (EXOSC7), also known as exosome component 7, is a protein encoded by the EXOSC7 gene. This protein is part of the exosome complex, possesses 3'->/5' exoribonuclease activity, and participates in multiple cellular RNA processing and degradation events. Within the nucleus, the RNA exosome complex participates in the proper maturation of stable RNA species and eliminates RNA processing byproducts and noncoding "universal" transcripts, thereby limiting or precluding their export to the cytoplasm. | |||||||||||||||||||||||||||||||||
| General Notes | 1. Strictly adhere to the specified incubation time and temperature to ensure accurate results. All reagents must be at room temperature (20-25°C) before use. Refrigerate reagents immediately after use. 2. Improper plate washing may result in inaccurate results. Ensure that all liquid in the wells is aspirated thoroughly before adding substrate. Do not allow the wells to dry out during incubation. 3. Remove any residual liquid and fingerprints from the bottom of the plate, as this will affect the OD value. 4. The substrate developer solution should be colorless or very light in color. Do not use substrate solution that has turned blue. 5. Avoid cross-contamination of reagents and specimens to prevent erroneous results. 6. Avoid direct exposure to strong light during storage and incubation. 7. Do not expose any reagents to bleaching solvents or the strong fumes emitted by bleaching solvents. Any bleaching agent will destroy the biological activity of the reagents in the kit. 8. Do not use expired products, and do not mix components with different product numbers and batches. 9. Recombinant proteins from sources other than the kit may not be compatible with the antibodies in this kit and will not be recognized. 10. If there is a possibility of disease transmission, all samples should be managed properly and samples and testing devices should be handled according to prescribed procedures. |
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| Storage Temp. | If the unopened kit is stored at 4°C, the shelf life is 6 months. | |||||||||||||||||||||||||||||||||
| Test Range | 0.156-10 ng/mL | |||||||||||||||||||||||||||||||||
| Applications | Tissue homogenates, cell lysates, and other biological fluids |
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4.2 ★★★★★
Based on 11 reviews
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Product Reviews
★★★★★ 5
Best right-handed trackball ever
This is the best pointing device I've ever used, hands-down. This is the second one I've purchased since I've managed to run out the last one in 4-5 years of very heavy usage. Here's what I like about it.
The real centerpiece of this trackball is the off-center ball. Now, this does make this definitely a right-handed trackball and lefties will probably feel left out, but putting the ball off-center matches the general distribution of your fingers over your hand. It's been to be maneuvered with your index through ring finger, and it is perfectly positioned to roll to any of those fingers as you need to use the scroll wheel or the right mouse button. Compared to a more omni-handed Kensington and the comfort difference is astounding. Likewise, for me, this is infinitely more comfortable than using my thumb for the trackball. The trackball pointing devices that use the thumb inevitably make my hand and thumb joint feel jammed up.
Also compared to Kensington, having a proper vertical scroll wheel is infinitely better than their "orbit ring" in my mind. The index finger quite naturally lines up with the wheel. The overall shape fits my hand comfortably and makes for easy long hours working or gaming.
Construction is solid and it's durable with good maintenance. As noted I used the previous one for at least 4 years, and used it quite heavily for general computer tasking, through EDA tools (Modelsim), through heavy gaming (including FPS and MMORPGs). If you are a button hog, then you might run out, but there's more than enough for me to use and i'm quite pleased with what I've been able to make it do.
Defictis: There are few. As noted, if you are a left handed computer pointer... you'll have to go with a mouse or a Kensington. If you've trained your right hand to point this may work for you. The wireless aspect of the device is not it's strongest feature and there have been a few rare moments when it seems to lose its connection with the base station. And wireless range is probably a little limited, though I've used it successfully several feet away from the base. It does have a few dropouts in motion further away though, so don't use this for a large presentation room or TV gaming.
But that's pretty much it. The sad thing is that Logitech doesn't seem to push this product into stores, so it's a bit hard to find on the shelf where you can do a touch fit. They've used this form factor for awhile though, so hopefully it'll continue to be useful in Amazon sales and make a comeback in a new product again soon. I"m tempted to buy another though to have in reserve in 4-5 years though!
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Reviewed in the United States on December 31, 2010
★★★★★ 5
Great Ergonomics
Color: Black
I had bought this keyboard back in 2012 when I had tendonitis and needed to make my entire work set-up more ergonomic. This keyboard helped immensely! This keyboard comes with the extra bottom attachment to lift the keyboard, but I always found it more comfortable to use the keyboard without it (maybe just add a wrist-rest if needed). I type pretty often and very quickly, and it was easy to adjust to the split layout (only took a couple hours to remember which keys to hit with which hand...). The quality of this keyboard is pretty decent - I would still be using this keyboard to this day, but it had one-too-many liquid spills back in 2020 to the point that certain keys would not function, so that was that.
I have used this keyboard for windows, linux, and mac (primarily mac) and can say that all the regular keyboard functions worked. All of the extras (i.e. 'media' buttons along the top and center) were inconsistent about working and depends on what system you are using. I didn't take too much time to force it into working, since I didn't really need those functions. I also travelled for work with this keyboard - although it's bulky, it does fit in the check-in bags, and the wired function is convenient since it doesn't require batteries.
Although I loved this keyboard dearly, when it met it's untimely death, I looked for a replacement and I was surprised at the price this keyboard is currently at. It's definitely not something I would be able to afford back when I was working barely above minimum wage... I can't promise if it'll be worth the cost as there are other alternatives that could work just as well at a lower price. However, my experience of this keyboard was definitely excellent and met my ergonomic needs.
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Reviewed in the United States on November 30, 2023
★★★★★ 5
Works for what I need it for
Color: Black
I've been using this type of keyboard for years. I remember back in the late 1990/early 2000s, I was working as a medical transcriptionist and my supervisor at the time was using a "curved" white Microsoft Natural Elite keyboard. I was still using the typical, out-of-the-box, straight keyboard. She gave me hers to try out for a while. At first, I HATED it. My typing speed was terrible, I was pressing every wrong key, etc. But after I got used to it, I realized there was no way I could ever go back to a straight keyboard. Now, all of these years later I'm still working as a medical transcriptionist and have graduated up to the Microsoft 4000. I still have problems using a straight keyboard - in fact, when I'm using my laptop for work I cannot use the laptop keyboard and have to hook up my own keyboard in order to transcribe.
Compared to all of the other "ergo" keyboards out there that I have tried (and I have tried quite a few), this is the best I've found. Now, I have never tried the Kinesis which I hear once you try and actually get used to you will never go back to anything else ever again and that it's the "best of the best." I have thought about trying one of those out, but I just cannot justify the cost. Perhaps if I was suffering from more carpal tunnel symptoms, more wrist pain, etc., I would give it a try, but as of now the Microsoft 4000 works for what I need it for. I am pairing it with an Anker wireless mouse, which took me a while to get used to but now I couldn't function without it! My sister was visiting me a few months ago and she was having a terrible time using my keyboard and mouse just to function on the internet and I then realized how somebody who isn't used to it feels. As I mentioned above, I've been using one of these "curved" split keyboards for nearly 20 years now, so I'm definitely used to it. With this keyboard, my hands feel like they are at a more natural position compared to a regular standard keyboard where they feel to be unnaturally squished close together.
When I worked in-house at a local clinic (I currently work from home), we had occupational therapists come over to evaluate our work stations, etc. I was told I have hyperreflexia and that the best position for this keyboard was to keep the lower bottom "riser" attached and to keep the top legs folded up so that my hands are sloping down from my wrist placement. That is the best position for me, but others may be different.
As I mentioned before, I have used this brand of keyboard for years, though I need replacements every six months or so as I am definitely NOT easy on my keyboards!! I always keep a spare one around which is good as the other day my Diet Coke spilled all over the one I was using. I thought I caught it early enough and got it all drained out, but nope, it was (and still remains) dead. So the spare I keep around does come in handy!!
All in all, this is a great keyboard for those of us who type on a regular basis. My only complaint (and this is not with EVERY Microsoft 4000 I've purchased, only a few) is that there are times when the space bar is a bit sticky. I pound the keys so hard while I'm typing that I generally don't notice this, but I have had a few that I have noticed it on. Otherwise, all keys seem to depress well. The letters on the "common" keys do wear off around four or five months, but that is something I could care less about as I don't ever look at my keyboard when I'm transcribing (though those that attempt to use my keyboard have commented on it)!!
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Reviewed in the United States on September 2, 2017
★★★★★ 5
Good keyboard but it takes time to adjust.
Color: Black, Color: Black
I had a prior version of this keyboard (the Microsoft Natural Ergonomic Keyboard 3000) for my office PC. It did admirably, but after a year or so, my wrists were starting to hurt IN SPITE OF the ergonomics helping. This one is better rated. So far, it has helped, but it took at least a week of use just for my hands to BEGIN to get used to the differences. I'd do a pro/con list, but I'm not sure there are any "cons" as compared to "issues to expect" if you buy this keyboard.
1. Expect a steep learning curve to get used to the layout. The "bend" in this keyboard is significant, and it will take time for your fingers to get used to hitting the right keys on a consistent basis. Give it one or two weeks of regular use before deciding if you like it or not. You can't know in a couple of days if it's helping you because you do need to re-learn your hand placement and fingering to some degree.
2. There is a physical break in the left and right hand keys. This "no man's land" means you need to unlearn using the wrong hand for the wrong keys because you will either miskey or just hit nothing when you go to use your right hand to it the G key (or a similar situation). Likewise, some of the keys have an odd shape that takes getting used to. I had considerable issue getting used to hitting V and B correctly. Again, this is all about relearning how to keyboard properly. My best tip is to avoid looking at the keyboard while typing. For me, looking at the keyboard made it harder compared to just backspacing and repeating until I got it right. Passwords are about the only thing where I still watch what keys I strike.
3. This keyboard is wider and "taller" than most. If you use a keyboard tray, clearing it might pose and issue (see next point below). You will likely have less room for your mouse as well, but I found it something I could live with.
4. The keyboard has three (maybe four) resting positions. There's a removable front skirt that elevates the front. There are the standard rear folding supports to elevate the rear. So, that's resting flat (none), rear elevated, front elevated, and both elevated. Lying flat, the keyboard works fine with my keyboard tray. Set the way I find it's most comfortable for me, I can only push the tray in halfway before the keyboard hits the desk. This isn't a deal breaker for me, so I'll live with it. You keyboard tray may need to allow at least 3 inches of vertical clearance to not have an issue with this keyboard.
I was divided on four or five stars, but since I need this more extreme curve to deal with wrist pain, it's not the product's fault, so I won't hold the awkwardness of it against the designers. It does what it's designed to do for a good reason. That comes with a trade off.
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Reviewed in the United States on January 3, 2017
★★★★★ 4
I was glad I bought it until four or five months later...
Color: Black
I am now about a week and a half into using this keyboard. It is the first time I have used an ergonomic split keyboard. I am a medical transcriptionist and type for a living. At first I found it awkward, I kept reaching too far for some keys, and it seemed that I was always missing the right-sided home row by one key. Now I find that I'm not thinking about the keyboard at all, which means it is working well for me. I find the key "touch" to be a little on the firm side, which means that I occasionally miss a letter because I didn't push hard enough, but I think over time I will adjust to that, too. This keyboard does not "click" when you type, which means it is generally quiet. Personally, I prefer the clicking, but for work, I wear a headset and don't hear the keyboard at all, so this is not an issue. Of course, with the need to press the keys harder, there is more thumping than I think I would normally hear when I type. As far as the alignment, it seems excellent. I have rheumatoid arthritis, which is well controlled, but I still have some stiffness and loss of range of motion in my fingers and wrists, and the angles of the keyboard seem to be perfect for me, and I don't feel as much strain as I used to in my wrists after working all day. There is one thing I wish was included, and that is a lighted keyboard. I have never had one, but I have worn the letters off the keys of several keyboards, plus it would just be helpful to be able to see the keys more easily for those times when you don't have bright light or so your eyes don't have to adjust to dim lighting after staring at your bright monitor screen for a long period of time. After less than two weeks, I can already see shiny spots on the space bar and some of the most-used keys, so I know the letters will start wearing off soon. Not a big deal, but a lighted keyboard would be a really nice feature, perhaps an option that could be offered in the future. If you are considering this type of keyboard, I think it is worth the minor aggravation of needing some time to get used to it. My impression is that my hands and wrists will be thankful I made this purchase. UPDATE JULY 22, 2015: The keyboard started acting strangely yesterday morning, some keys not working at all, some keys creating the wrong letter or digit, and the period key seemed to be stuck. I tried to file a claim using the extended warranty, but because it was still under the manufacturer's warranty, they told me to contact Microsoft, and they gave me the wrong phone number for Microsoft. Microsoft wanted the keyboard to be shipped back to them in California, and they would send a replacement AFTER they received this one. I use the keyboard for work, and I don't have a week or more to wait for a replacement. I asked for my money back, which they agreed to do, BUT they then sent me on a wild goose chase trying to make that happen. It seemed that no one with Microsoft had any idea of how to help me and they didn't seem interested at all in helping me, although one person I spoke to at one of the wrong numbers I was given was very kind and helpful in spite of my anger and frustration (thanks, Sachin!), but he couldn't help me either, and he said I needed to contact Amazon. This was definitely NOT Amazon's problem, but you know what? They made it right. No arguments, no questions, no lengthy explanations, no making me feel like I was trying to take advantage of them. I asked for their help, and they apologized for the troubles I had experienced and sent a new keyboard. I have heard people complain about Amazon, but as far as I'm concerned, they are AWESOME. Microsoft and Square Trade Warranties not so much. This keyboard should have lasted longer than a few months, and Microsoft's customer service was horrible when I needed help, so when I have to purchase another keyboard, I probably won't buy another Microsoft product. That said, I have been very happy with the layout of the keyboard and I wish it hadn't malfunctioned so quickly.
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Reviewed in the United States on March 25, 2015
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