Pay in installments of $46.03 with
,
and
Shipping Estimate
USA
- USA
- CAN
- USA
- CAN
Ships within 48 hours · Estimated delivery Aug 16 - Aug 21
Promo Codes Available:
For Your Every Summer RSVP, with Code: SUMMER15
Description
Mouse CRAMP ELISA KitProduct Specification Usage Experimental equipment required for the experiment: 1. Microplate reader (450nm) 2. High precision pipette and gun tips: 0. 5 10uL, 5 50uL, 20 200uL, 200 1000uL 3. 37 constant temperature box 4. Distilled water or deionized water Sample processing and requirements: Serum: Place the whole blood sample collected in the serum separation tube at room temperature for 2 hours or at 4 overnight, then centrifuge at 1000g for 20
Product Specification
| Usage |
Experimental equipment required for the experiment: 1. Microplate reader (450nm) 2. High-precision pipette and gun tips: 0.5-10uL, 5-50uL, 20-200uL, 200-1000uL 3. 37℃ constant temperature box 4. Distilled water or deionized water Sample processing and requirements: Serum: Place the whole blood sample collected in the serum separation tube at room temperature for 2 hours or at 4℃ overnight, then centrifuge at 1000×g for 20 minutes, and take the supernatant, or store the supernatant at -20℃ or -80℃, but avoid repeated freezing and thawing. Plasma: Collect the specimen using EDTA or heparin as an anticoagulant. Centrifuge the specimen at 1000 × g for 15 minutes at 2-8°C within 30 minutes of collection. The supernatant can be assayed or stored at -20°C or -80°C, but avoid repeated freezing and thawing. Tissue homogenization: Rinse the tissue with pre-chilled PBS (0.01M, pH 7.4) to remove residual blood (lysed red blood cells in the homogenate will affect the measurement results). Weigh the tissue and mince it. Add the minced tissue to the appropriate volume of PBS (generally a 1:9 weight-to-volume ratio, e.g., 1 g of tissue sample to 9 mL of PBS. The specific volume can be adjusted according to experimental needs and recorded. It is recommended to add protease inhibitors to the PBS) in a glass homogenizer and grind thoroughly on ice. To further lyse tissue cells, the homogenate can be sonicated or repeatedly frozen and thawed. Finally, centrifuge the homogenate at 5000 × g for 5-10 minutes, and the supernatant can be assayed. Cell Lysis Buffer: Gently wash adherent cells with ice-cold PBS, then trypsinize and collect cells by centrifugation at 1000×g for 5 minutes. Suspension cells can be collected directly by centrifugation. Wash collected cells three times with ice-cold PBS and resuspend in 150-200 μL of PBS per 1×10^6 cells (it is recommended to add protease inhibitors to the PBS; if the cell count is very low, reduce the PBS volume appropriately). Disrupt the cells by repeated freeze-thaw cycles or sonication. Centrifuge the extract at 1500×g for 10 minutes at 2-8°C, and remove the supernatant for analysis. Cell Culture Supernatant: Centrifuge at 1000×g for 20 minutes. Remove the supernatant for analysis or store at -20°C or -80°C, avoiding repeated freeze-thaw cycles. Other biological fluids: Centrifuge at 1000xg for 20 minutes, remove the supernatant, and test. Pre-test preparation: 1. Remove the test kit from the refrigerator 10 minutes in advance and equilibrate to room temperature. 2. Prepare the standard gradient working solution: Add 1 mL of universal diluent to the lyophilized standard, let it stand for 15 minutes to completely dissolve, then gently mix (concentration is 20 ng/mL). Then dilute to the following concentrations: 20 ng/mL, 10 ng/mL, 5 ng/mL, 2.5 ng/mL, 1.25 ng/mL, 0.625 ng/mL, 0.3125 ng/mL, and 0 ng/mL. Serial dilution method: Take 7 EP tubes and add 500 μL of universal diluent to each tube. Pipette 500 μL of the 20 ng/mL standard working solution into the first EP tube and mix thoroughly to make a 10 ng/mL standard working solution. Repeat this procedure for subsequent tubes. The last tube serves directly as a blank well; there is no need to aspirate the liquid from the penultimate tube. See the figure below for details. 3. Preparation of Biotinylated Antibody Working Solution: 15 minutes before use, centrifuge the concentrated biotinylated antibody at 1000×g for 1 minute. Dilute the 100× concentrated biotinylated antibody to a 1× working concentration using universal diluent (e.g., 10µL concentrate + 990µL universal diluent). Prepare immediately before use. 4. Prepare the enzyme conjugate working solution: 15 minutes before use, centrifuge the 100× concentrated enzyme conjugate at 1000×g for 1 minute. Dilute the 100× concentrated HRP enzyme conjugate to a 1× working concentration with universal diluent (e.g., 10 μL of concentrate + 990 μL of universal diluent). Prepare immediately. 5. Prepare the 1× wash solution: Dispense 10 mL of 20× wash solution into 190 mL of distilled water (concentrated wash solution removed from the refrigerator may crystallize; this is normal. Allow to stand at room temperature until the crystals have completely dissolved before preparing). Procedure: 1. Remove the desired strips from the aluminum foil bag after equilibration at room temperature for 10 minutes. Seal the remaining strips in a ziplock bag and return to 4°C. 2. Sample addition: Add 100 μL of sample or standard of varying concentrations to the corresponding wells. Add 100 μL of universal diluent to the blank wells. Cover with a film and incubate at 37°C for 60 minutes. (Recommendation: Dilute the sample to be tested at least 1-fold with universal diluent before adding it to the ELISA plate. This will reduce the impact of matrix effects on the test results. The sample concentration should be multiplied by the corresponding dilution factor when calculating the final sample concentration. It is recommended to run replicates for all test samples and standards.) 3. Add Biotinylated Antibody: Remove the ELISA plate and discard the liquid without washing. Add 100 μL of Biotinylated Antibody Working Solution directly to each well. Cover with a film and incubate at 37°C for 60 minutes. 4. Wash: Discard the liquid and add 300 μL of 1x Wash Solution to each well. Let stand for 1 minute, shake off the wash solution, and pat dry on absorbent paper. Repeat this process three times (a plate washer can also be used). 5. Add Enzyme Conjugate Working Solution: Add 100 μL of Enzyme Conjugate Working Solution to each well. Cover with a film and incubate at 37°C for 30 minutes. 6. Washing: Discard the liquid and wash the plate five times as in step 4. 7. Adding substrate: Add 90 μL of substrate (TMB) to each well, cover with a sealing film, and incubate at 37°C in the dark for 15 minutes. 8. Adding stop solution: Remove the ELISA plate and add 50 μL of stop solution directly to each well. Immediately measure the OD value of each well at a wavelength of 450 nm. Calculating experimental results: 1. Calculate the average OD value of the standard and sample replicates and subtract the OD value of the blank well as a correction factor. Plot the standard curve of the four-parameter logistic function on double-logarithmic graph paper, with concentration as the horizontal axis and OD value as the vertical axis. 2. If the sample OD value is higher than the upper limit of the standard curve, dilute the sample appropriately and retest. Multiply the sample concentration by the corresponding dilution factor. |
|||||||||||||||||||||||||||||||||
| Theory | This kit uses a double-antibody sandwich enzyme-linked immunosorbent assay (ELISA). Sample, standard, biotin-labeled detection antibody, and HRP conjugate are sequentially added to microwells pre-coated with a capture antibody against cathelin-related antimicrobial peptides (CRAMP). After incubation and washing, the sample is developed using the substrate TMB. TMB is converted to blue by HRP peroxidase and to yellow by acid. The intensity of the color is positively correlated with the amount of cathelin-related antimicrobial peptides (CRAMP) in the sample. The absorbance (OD) is measured at 450 nm using a microplate reader to calculate the sample concentration. | |||||||||||||||||||||||||||||||||
| Source | Mouse | |||||||||||||||||||||||||||||||||
| Synonym | Mouse Cathelin-related antimicrobial peptides ELISA Kit | |||||||||||||||||||||||||||||||||
| Detection Type | Double antibody sandwich method | |||||||||||||||||||||||||||||||||
| Composition |
|
|||||||||||||||||||||||||||||||||
| Background | Cathelin-related antimicrobial peptide (CRAMP) is the only identified murine cathelicidin. CRAMP has been shown to possess antimicrobial and angiogenic activities. It also induces calcium mobilization and MAPK activation in monocytes. | |||||||||||||||||||||||||||||||||
| General Notes | 1. Strictly adhere to the specified incubation time and temperature to ensure accurate results. All reagents must be at room temperature (20-25°C) before use. Refrigerate reagents immediately after use. 2. Improper plate washing may result in inaccurate results. Ensure that all liquid in the wells is aspirated thoroughly before adding substrate. Do not allow the wells to dry out during incubation. 3. Remove any residual liquid and fingerprints from the bottom of the plate, as this will affect the OD value. 4. The substrate developer solution should be colorless or very light in color. Do not use substrate solution that has turned blue. 5. Avoid cross-contamination of reagents and specimens to prevent erroneous results. 6. Avoid direct exposure to strong light during storage and incubation. 7. Do not expose any reagents to bleaching solvents or the strong fumes emitted by bleaching solvents. Any bleaching agent will destroy the biological activity of the reagents in the kit. 8. Do not use expired products, and do not mix components with different product numbers and batches. 9. Recombinant proteins from sources other than the kit may not be compatible with the antibodies in this kit and will not be recognized. 10. If there is a possibility of disease transmission, all samples should be managed properly and samples and testing devices should be handled according to prescribed procedures. |
|||||||||||||||||||||||||||||||||
| Storage Temp. | If the unopened kit is stored at 4°C, the shelf life is 6 months. | |||||||||||||||||||||||||||||||||
| Test Range | 0.312-20 ng/mL | |||||||||||||||||||||||||||||||||
| Applications | Serum, plasma, tissue homogenate, cell lysate, cell culture supernatant and other biological fluids |
Shipping Notes
- Free Standard Shipping on $100+ Orders to the USA.
- Except Preorder products are shipped in 48 hours.
- Delivery to the USA:
- Standard Shipping : 3-10 business days
- If time is of the essence, please consider selecting expedited delivery for faster service.
Exchange/Return Notes
- We offer a 30-day return/exchange service after receiving.
- Final sale items are not eligible for returns or exchanges.
- To process your return/exchange, please contact us at [email protected]
- Please click here for more details>>> Return & Exchange Policy
4.8 ★★★★★
Based on 15 reviews
Sort
Product Reviews
★★★★★ 5
😍😍 BEST DEBUT NOVEL EVER
Format: Paperback
I’m having trouble finding accurate words to describe the way this book made me feel, but I am going to do my best.
To start off with basic elements, the character and world building are phenomenal. I feel a strong bond to not only the two main characters, Ara and Rogue, but to each and every character introduced throughout the book. The author did a stellar job of giving each of them unique personhood. All of the scenes are beautifully described. So much so that throughout the entirety of the book, I could see every scene: the towns, the castles, the meadows, the landscape. I have had difficulty with this and with distinguishing between outlying characters while reading in the past, but I did not have to think to remember details of world or character building because they flowed naturally within the story and were described well. I have read book series before that made me want to be a part of that world, but I actually felt like I got to step into Auryna and Ravaryn!
The plot twists!! Although this is not a suspense novel, it still had me on a rollercoaster of emotions and on the edge of my seat from beginning to end. I haven’t cried actual tears over a book since I was in high school (and I’ve read a LOT). This book finally broke the floodgates in the final few chapters. Multiple times. And we love a good cliffhanger. It truly made me FEEL.
THE SPICE is a solid 3.5/5. Some of the scenes had me flushed, some had me taking notes, some just had my jaw slack and my mouth hanging open. Bravo, JD Linton, bravo.
The relationships: friendships, family, romantic, ALL of the relationships in this book have so much meaning. The author does a great job at making you feel the love, the anger, the peace, the frustrations, the safety, the familiarity, etc. between the characters.
Ara and Rogue. I can not say enough and I also do not want to say too much. Just know that I feel like I know them both, to their core. I know what their childhood looks likes, their darkest moments, their biggest fears, their dreams and passions, what they want in life… The POV switches were seamless. I am so happy this author decided to let us see from both sets of eyes.
I can not wait for book two after that cliffhanger. And there is SO much potential for at least one prequel, I can’t wait to see where this author goes! I hope this series continues and flourishes. Fingers crossed!
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on December 5, 2022
★★★★★ 5
Amazing!
Format: Kindle, Format: Kindle
This book was phenomenal, I devoured it within a few days! For this being a debut novel, it is fantastic and I would’ve thought the author was a seasoned author. I have zero complaints about this book.
Let me start by saying that the world building was phenomenal. I could picture everything in my head because of how detailed it was — that’s how good it was written. And I absolutely love the “captive/captor” trope so much, it’s become one of my favorite tropes, so I was pleasantly surprised to see that this book had that.
I loved the banter between Rogue and Ara — they’re both snarky and witty, plus with the romantic tension, it made the dialogue that much better. Speaking of romantic tension, yes there is spice but not so much of it that it overrides the plot, which I loved. For me, this would probably be on the 3/5 level of spice.
This book had a ton of plot twists and I thoroughly enjoyed it.
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on November 13, 2024
★★★★★ 4
High on Tropes and Satisfaction
Format: Kindle
This is a great Romantasy book full of action, adventure, and everything you look for in this genre.
I won’t lie: it does kinda feel like the author found every common trope from every successful book of this kind and threw them all into this novel. But if it ain’t broke, don’t fix it. Especially in romance, there’s a large audience who has specific expectations, and they want them every time. Nothing wrong with that and many times I’m one of them.
I have no idea what defines a spoiler honestly, so
spoiler alert!!!!!!!
Tropes include:
Only one bed at the inn/bar
Dissatisfaction with life before hunk appears
Lost royalty
The chosen one
Montage of dress up time followed by shocked hunk
Forbidden romance between two from rival peoples
Power that cannot be controlled, simply guided/asked
Gathering intel at the inn/bar
FMC who knows how to fight/use weapons well
There’s probably more but no need to list them all.
Good story and I would recommend!
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on June 14, 2024
★★★★★ 5
Astonishing, Fun, Entertaining, Fantastic
Format: Kindle
I consider The Martian my favorite fictional novel of the last 15-20 years. The movie was incredible in that they actually followed the book closer than 99% of other films based on books. It remains my favorite movie of the last 15 years or so as well. I don't know anyone (personally) that loves either of them as much as I do.
With that said, I was REALLY looking forward to Artemis. It was good...but, it was certainly not in the same caliber as The Martian was (at least not for me). I enjoyed it a lot, however and appreciated how author Andy Weir chose to go in a completely different direction and not just rehash another similar story, which I am certain would have been great as well.
As a result, I was cautious regarding Project Hail Mary. It sounded a little too close to The Martian, but yet, also different in that the circumstances simply could not be more opposite and the stakes so much higher. I'm trying to figure out the best way to summarize without giving too much away from this utterly compelling novel. As I read several reviews, I noticed a recurring theme: SCIENCE. Lots and LOTS of science. Holy cow, they were right. Many years ago I read Apollo 13 and Jim Lovell and his co-writer, try as they might, simply could not dumb down Orbital Mechanics anywhere near enough for me to have even a minor clue as to what they were attempting to say...I just skipped 90% of it and hoped that the sentences written afterwards, would help to make sense of what I had just skimmed over. I'm a lot of things, but a math wizard is definitely not one of them. Michael Crichton (Jurassic Park) had an amazing talent for dumbing-down the science of what he was trying to explain in ways that genuinely made sense (most of the time). Not everyone has this talent, and I would say Andy Weir falls squarely in between. He's certainly better than Jim Lovell, but not quite as good as Crichton. But then again, outside of a science textbook, I haven't really read anything with quite as MUCH science as Project Hail Mary. So maybe he's just as good, but he just puts more science into his books than Crichton, maybe that's it...? Either way, be prepared for a lot of astonishingly interesting science within the pages of this novel...and I DO mean a LOT. I don't say this to make you wary or steer you away...on the contrary, Andy Weir has a special talent for making hard science truly entertaining.
The book opens with an absolutely amazing and frightening premise: an astronaut awakes from an induced coma to find the only other two people on board have died at some point along their journey...but it gets worse. He has no idea who he is, or why he's on the ship, and oh yeah, they look to be a long way from home. A really, REALLY long way from home. In fact, the sun he sees isn't actually OUR sun at all. He's managed to leave our solar system entirely. And he has no idea why.
((Minor Spoilers)) The book goes through some clever flash-backs, which set the stage for why the mission happens, and slowly, carefully explains how they managed to get so far away from earth in such a short amount of time. Basically, earth's sun seems to be dying. At the rate of decay, we have maybe 19 years left before the gradual cooling has catastrophic consequences resulting in the death of billions (best guess). Why the sun is dimming is quite the conundrum in the first place. Turns out it really isn't dying, it's being killed by an outside source...which turns out to be easily the greatest find in history. It's alien life, and they are using the sun for food, essentially. It's alien life, but not intelligent life. But still, wow! ALIENS, right???
After this monumental discovery, and some tremendous research done by the most improbable scientist, the investigation into what is happening and why and what to do about it expands exponentially to other nations in order to pool all the resources possible to hopefully save the sun, and by extension, the human race as well. They learn. A LOT. A plan is put together, and with the help of the newly discovered microscopic alien life, which can also double as a power source (along with a few other nifty surprises), they begin to create one last, Hail Mary that could very well be the last chance we might have to save earth. It's audacious. It's dangerous, and it is absolutely critical that it succeed.
As our astronaut's memory slowly unravels, so does his identity: Ryland Grace. He's a teacher on earth. Just a science teacher. Not even a college professor. He's amazingly smart, though. But he's no astronaut...and certainly not one who would volunteer to go on a one-way mission to another solar system to "try" and save humanity. Yet here he is. Alone. light years from earth, trying to solve the biggest riddle in all of human history. Ryland accepts his situation, such as it is, with relative indifference (for the most part). It doesn't matter HOW he got here. He's here now and he may as well use that time to be as productive as possible, right? Along the way, he unravels even more information regarding the microscopic alien life which is slowly dimming our sun during some additional flashbacks. The aliens, dubbed, "Astrophage" are quite the galactic plague as it turns out. Stars all over the galaxy are also losing their light, all due to the little buggers. All that is, except one particular star named, Tau Ceti. Now why would that one star be unaffected by Astrophage, when every single star around it has been affected to some degree. The plan is to go there and figure it out and send the information back, hopefully in time to save the sun before the damage to earth is beyond repair.
There is an incredible amount of stuff going on. The story switches from Tau Ceti to flashbacks of how the whole mission was planned and implemented (which is VERY entertaining, especially Director Stratt, who may actually be my favorite character in the entire novel). Weir is becoming quite adept at building tension, and abruptly switching the story from Tau Ceti back to earth and building more of the backstory then switching back to Tau Ceti. Keeping it all in check and most importantly, interesting all while mixing in a healthy dose of science, which I am to understand is pretty much all genuine, is quite the juggling act. I have long known science can be astronomically entertaining (see what I did there?) when done right...but unfortunately very few people in a position to teach science actually know the best way to create that interest in others. I can say without reservation, Andy Weir definitely knows how to do it...at least in written form.
There is so much I want to say more regarding this truly phenomenal story, but I simply cannot without ruining a lot of the fun and surprises revealed along the way...and it is killing me to keep it locked in. Though I labeled a spoiler warning earlier, I don't think it gave away any more than what the author himself has revealed in interviews he has done regarding the book, and what you can glean from reading the summary here and just a couple other reviews. Tying all of that science together is truly astonishing to me. The creativity to put it into a novel that is remarkably exciting to read is nothing more than incredible talent. Kudo's to Andy Weir for not just hitting a home run, Project Hail Mary is a Grand Slam all the way. I truly did not want this story to end. By the way, I enjoyed the ending quite a bit. I don't know if everyone will. But it was fine for me. I think the ending screams "sequel" at some point too. A lot was left open-ended (IMO) and I wouldn't mind reading a follow-up to this. It doesn't HAVE to happen, but there are a lot of ways where the story could go if Andy chose to do it. Just sayin'.
Just run out and buy this book.
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on May 10, 2021
★★★★★ 5
Wonderful
Format: Kindle
The amount of detail in this book is so interesting and the specifics of so much theoretical ideas revolving around true ideas makes it so fun to read. The writer does a great job and describing every situation enough where you get the point but not too much to try to bore you . The book is very easy to follow, keeps you on your toes, was pretty funny to me, and truthfully just a great book for anyone!
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on May 20, 2026