Pay in installments of $208.38 with
,
and
Shipping Estimate
USA
- USA
- CAN
- USA
- CAN
Ships within 48 hours · Estimated delivery Aug 10 - Aug 15
Promo Codes Available:
For Your Every Summer RSVP, with Code: SUMMER15
Description
Mouse Single-Cell BCR FL Amplification Kit (BD Platform)Product Specification Background This product is mainly used for the determination of the paired full length VDJ sequences in mouse derived single B cells. This product is compatible with the BD Rhapsody single cell sequencing platform. It adopts optimized target amplification primer sequences and the most suitable enzyme reaction system, which can effectively amplify and detect the light and heavy chain sequence information of mouse derived single B
Product Specification
Background
This product is mainly used for the determination of the paired full-length VDJ sequences in mouse-derived single B cells. This product is compatible with the BD Rhapsody single-cell sequencing platform. It adopts optimized target amplification primer sequences and the most suitable enzyme reaction system, which can effectively amplify and detect the light and heavy chain sequence information of mouse-derived single B cells, including IGHA, IGHG, IGHM, IGKC, IGLC, etc.
Components
Component |
Size |
Volume |
TSO |
1 |
30uL |
1M MgCl2 |
1 |
15uL |
Hybridization Buffer |
4 |
1.6mL |
Extension Enzyme |
1 |
70uL |
Extension Buffer |
1 |
160uL |
Extension Primers |
1 |
30uL |
dNTP |
1 |
200uL |
Nuclease Free Water |
2 |
1mL |
PCR MasterMix |
1 |
1.2mL |
Bead RT/PCR Enhancer |
1 |
200uL |
VDJ Universal Oligo N1 |
1 |
65uL |
VDJ Universal Oligo N2 |
1 |
30uL |
BCR N1 Primer |
1 |
16uL |
BCR N2 Primer |
1 |
40uL |
Elution Buffer |
2 |
1.8mL |
Protocol
Refer to the BD Rhapsody full-length VDJ library construction method.
File name: BD Rhapsody™ System Library Preparation Protocol TCR/BCR Full Length and mRNA Whole Transcriptome Analysis (WTA)
Document number: 23-24017(03)
Download link: https://scomix.bd.com/hc/en-us/articles/9285990045197-VDJ-full-length-TCR-BCR-assay-protocols-Human
Note: In this kit, the volume of Bead RT/PCR Enhancer is 40 µL, and the corresponding volume of Nuclease-Free Water is 45.2 µL.
Shipping Notes
- Free Standard Shipping on $100+ Orders to the USA.
- Except Preorder products are shipped in 48 hours.
- Delivery to the USA:
- Standard Shipping : 3-10 business days
- If time is of the essence, please consider selecting expedited delivery for faster service.
Exchange/Return Notes
- We offer a 30-day return/exchange service after receiving.
- Final sale items are not eligible for returns or exchanges.
- To process your return/exchange, please contact us at [email protected]
- Please click here for more details>>> Return & Exchange Policy