SKU: 84930630449

Human IL-1β High Sensitivity ELISA Kit

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Description

Human IL-1β High Sensitivity ELISA KitProduct Specification Description Detection Principle: This kit uses double antibody sandwich ELISA technology. Specific anti human il 1 Capture antibody was pre coated on a high affinity microplate. Add the standard and the sample to be tested into the wells of the enzyme plate in turn, shake well and mix well, and then place it at room temperature for 2 hours of incubation process. The il 1&beta in the sample; Binding to solid phase antibodies.

Product Specification

Description

Detection Principle:

This kit uses double antibody sandwich ELISA technology. Specific anti-human il-1β Capture antibody was pre coated on a high affinity microplate. Add the standard and the sample to be tested into the wells of the enzyme plate in turn, shake well and mix well, and then place it at room temperature for 2 hours of incubation process. The il-1&beta in the sample; Binding to solid-phase antibodies. After thorough washing to remove free and unbound components, biotinylated detection antibody was added for incubation. After washing again, streptavidin HRP (sa-hrp) labeled with horseradish peroxidase was added. After washing, signal enhancer was added for incubation. After washing to remove unbound material, sa-hrp was added again. After washing again, TMB chromogenic substrate was added and incubated at room temperature in the dark to develop color. The depth of color reaction and il-1&beta in samples; There is a positive correlation between the concentration of. Add stop solution to stop the reaction, and use a microplate reader to measure the absorbance value at 450 nm detection wavelength (correction wavelength 570-630 nm).

Detection Type: Double antibody sandwich method

Form: pre coated 96 well plate

Test Sample Type: cell supernatant, serum, plasma

Loading Amount: 100 μ L

Kit Components: Pre coated 96 well plates, standards, il-1β One copy of detection antibody, standard dilution, detection buffer, signal enhancer, signal enhancer dilution, TMB chromogenic substrate, washing solution, termination solution, sa-hrp, plate sealing membrane and instructions.

Sensitivity: 0.02pg/ml

Detection Range: 0.19-12 pg/ml

Recovery Range: 93-112%

Storage Method: 2-8 ℃

Standard Curve:

Background:

Interleukin 1 (IL-1) is a 17kDa extracellular polypeptide, which is divided into il-1α and il-1β two proteins. Il-1β is a protein precursor secreted by activated macrophages, which is secreted by caspase1 (casp1/ice)The activated form produced by hydrolysis. This cytokine is an important mediator of the inflammatory response and is involved in many cellular activities, including cell proliferation, differentiation and apoptosis. IL-1 is an important family of proteins, which are derived from bioactive monocytes and participate in inflammatory and immune responses. Il-1β Elevated expression can produce a variety of different autoinflammatory syndromes, the most significant of which is the monogenic condition known as caps, which is due to the triggering of il-1β It is caused by mutations in the processed inflammasome receptor NLRP3. This kit is used to detect human il-1β Of the mature form and the uncleaved precursor form.

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SKU: 84930630449

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Reepeat
Birmingham, US
★★★★★ 5
Most durable fetch balls I know of.
Size: 2-pack, Style: Duo
These balls are the biggest balls of them all. Well, not the biggest, but the best. Every other ball she would chew up in short order and I'd be throwing her a floppy piece of rubber. She just wants to fetch, but if it's not bouncing, she can't catch it on the bounce. Got these balls in July and no damage to them. I just ordered another pair, but only because the other two somehow got stuck up in trees. We have some very old and very large oak trees so not so easy to retrieve. Hope they somehow drop someday. But in the meantime, Lassie will be happy with her new ones. Very highly recommended!
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Reviewed in the United States on October 28, 2025
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Blonde Aggie
Cuba, US
★★★★★ 5
Works great for scent work! Bounce is great for air retrieve!
Size: 2-pack, Style: Duo, Size: 2-pack, Style: Duo
My dog doesn’t know he loves them yet but I do! They are a little softer so when we do air retrieve, I don’t have to worry as much about his teeth! I’m hoping he will learn to find them by smell vs sight. We often play in the evenings and he tends to loose his ball. Love chuck it! Right now, we have about 10 chuck it balls! The tennis balls don’t work for air retrieve! Chuckit does!!! Love the thrower too! Saves my arm!!!!
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Reviewed in the United States on August 6, 2025
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None of your business
Cuba, US
★★★★★ 3
I guess the smell is offputting
Size: 2-pack, Style: Duo
My dog loves chocolate stuff. But for some reason, wasn’t a huge fan of these balls. She played with them a little bit, but they’re not her favorite.
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Reviewed in the United States on December 30, 2025
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Stacie☺
Whiting, US
★★★★★ 5
Fun solid ball
Style: Recharges, Size: Medium, Style: Recharges, Size: Medium
Great squeaky balls! Glow doesn’t last for a long time, but they go far in the Chuck it. Solid ball, my dog doesn’t seem to destroy them.
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Reviewed in the United States on February 11, 2026
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Jennifer Gugala
Port Orchard, US
★★★★★ 5
Great ball!
Style: Recharges, Size: Medium
The dog loves chasing it! Walks around sqeeking it all the time. And bonus, I can see it in the middle of the floor when I get up in the middle of the night so I won't unknowing step on it.
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Reviewed in the United States on May 5, 2026

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