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Description
Anti-Human IgG Fc Acceptor BeadsProduct Specification Stability & Storage Store at 2 8C, protected from light; product shelf life is 12 months. Background Homogeneous Immuno Chemiluminescence Assay (HICA) is a homogeneous immunoassay method based on energy transfer between donor beads and acceptor beads at close proximity, resulting in luminescence. Components Specification Fill Volume 250 g 50 L 5 mg 1 mL 25 mg 1 mL x 5 Protocol ```html Required Reagents Name Catalog No. Anti Human
Product Specification
| Stability & Storage | Store at 2-8°C, protected from light; product shelf life is 12 months. |
Background
Homogeneous Immuno Chemiluminescence Assay (HICA) is a homogeneous immunoassay method based on energy transfer between donor beads and acceptor beads at close proximity, resulting in luminescence.
Components
Specification |
Fill Volume |
250 μg |
50 μL |
5 mg |
1 mL |
25 mg |
1 mL x 5 |
Protocol
```html
【Required Reagents】
Name |
Catalog No. |
| Anti-Human IgG Fc Acceptor Beads | UA086097 |
| Streptavidin Donor Beads | UA086104 |
| Universal Buffer 1 | UA086113 |
【Detection Procedure (Reference)】
Procedure |
Procedure 1 (37°C Rapid Detection) |
Procedure 2 (Room Temperature Detection) |
Step 1: |
4μL Tag1-M1 +4μL Tag2-M2+ 6μL Acceptor Beads,Light-protected/Green light |
4μL Tag1-M1 +4μL Tag2-M2+ 6μL Acceptor Beads,Light-protected/Green light |
Incubation |
37°C shaking incubation for 20 minutes,Light-protected/Green light | Room temperature incubation for 60 minutes,Light-protected/Green light |
Step 2: |
Add 6μL Donor Beads,Light-protected/Green light |
Add 6μL Donor Beads,Light-protected/Green light |
Incubation |
37°C shaking incubation for 10 minutes,Light-protected/Green light |
Room temperature incubation for 30 minutes,Light-protected/Green light |
Reading |
Instrument reading |
Instrument reading |
【Performance Validation】
•Sample Preparation:
Biotinylated human IgG (Bio-hIgG) was pre-diluted to 15μg/mL (100nM) using Universal Buffer 1 as the stock solution, followed by serial dilution as below:
ID |
Final Concentration (nM) |
Universal Buffer 1 Volume (μL) |
High Concentration Add Volume (μL) |
C12 |
1.0E+01 |
210 |
90μL stock solution |
C11 |
3.0E+00 |
210 |
90μL C12 |
C10 |
1.0E+00 |
180 |
90μL C11 |
C9 |
3.0E-01 |
210 |
90μL C10 |
C8 |
1.0E-01 |
180 |
90μL C9 |
C7 |
3.0E-02 |
210 |
90μL C8 |
C6 |
1.0E-02 |
180 |
90μL C7 |
C5 |
3.0E-03 |
210 |
90μL C6 |
C4 |
1.0E-03 |
180 |
90μL C5 |
C3 |
3.0E-04 |
210 |
90μL C4 |
C2 |
1.0E-04 |
180 |
90μL C3 |
C1 |
0 |
180 |
/ |
•Detection Reagent Preparation:
Name |
Preparation Concentration |
Diluent |
| Anti-Human IgG Fc Acceptor Beads | 25 μg/mL |
Universal Buffer 1 |
| Streptavidin Donor Beads | 25 μg/mL |
Universal Buffer 1 |
•37°C Incubation Mode Results:
Max signal: 4772579
Min signal: 856
EC50= 0.171 nM
•Room Temperature Incubation Mode Results:
Max signal: 1912242
Min signal: 375
EC50= 0.155 nM
```Guidelines
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