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Description
ROBO1 Recombinant Rabbit mAb (S-2931-169)Product Specification Host Rabbit Antigen ROBO1 Synonyms Roundabout homolog 1; Deleted in U twenty twenty; H Robo 1; DUTT1 Immunogen Synthetic Peptide Location Cell membrane Accession Q9Y6N7 Clone Number S 2931 169 Antibody Type Recombinant mAb Isotype IgG Application WB, IHC P, ICC Reactivity Hu, Ms, Rt Positive Sample HepG2, PANC 1, HeLa, BxPC 3, A549, U 2 OS, mouse E14 embryo, rat E14 embryo Purification Protein A Concentration 0. 5 mg ml
Product Specification
| Host | Rabbit |
| Antigen | ROBO1 |
| Synonyms | Roundabout homolog 1; Deleted in U twenty twenty; H-Robo-1; DUTT1 |
| Immunogen | Synthetic Peptide |
| Location | Cell membrane |
| Accession | Q9Y6N7 |
| Clone Number | S-2931-169 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | WB, IHC-P, ICC |
| Reactivity | Hu, Ms, Rt |
| Positive Sample | HepG2, PANC-1, HeLa, BxPC-3, A549, U-2 OS, mouse E14 embryo, rat E14 embryo |
| Purification | Protein A |
| Concentration | 0.5 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| WB | 1:1000 | Hu, Ms, Rt |
| IHC-P | 1:500 | Hu |
| ICC | 1:500 | Hu |
Background
ROBO1 is a ~165 kDa single-pass transmembrane receptor of the immunoglobulin superfamily whose extracellular region contains five Ig-like domains and three fibronectin repeats that bind the secreted SLIT1/2 ligands, while its cytoplasmic tail carries four conserved CC motifs (CC0-CC3) that recruit adaptors such as srGAPs, Ena/VASP and DCC to transduce repulsive or attractive signals regulating axon guidance at the CNS midline, neuronal precursor migration, angiogenesis and even tumor metastasis by modulating Rho-family GTPases and actin cytoskeleton dynamics.
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