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Description
Human IFIT2 ELISA KitProduct Specification Usage Required experimental equipment: 1. Microplate reader (450nm) 2. High precision pipettes and pipette tips: 0. 5 10uL, 5 50uL, 20 200uL, 200 1000uL 3. 37C incubator 4. Distilled or deionized water Sample preparation and requirements: Tissue homogenization: Rinse the tissue with pre chilled PBS (0. 01M, pH 7. 4) to remove residual blood (lysed red blood cells in the homogenate will affect the measurement results). Weigh and
Product Specification
| Usage | Required experimental equipment: 1. Microplate reader (450nm) 2. High-precision pipettes and pipette tips: 0.5-10uL, 5-50uL, 20-200uL, 200-1000uL 3. 37°C incubator 4. Distilled or deionized water Sample preparation and requirements: Tissue homogenization: Rinse the tissue with pre-chilled PBS (0.01M, pH 7.4) to remove residual blood (lysed red blood cells in the homogenate will affect the measurement results). Weigh and mince the tissue. Add the minced tissue to the appropriate volume of PBS (generally a 1:9 weight-to-volume ratio, e.g., 1g of tissue sample to 9mL of PBS. The specific volume can be adjusted according to experimental needs and recorded. It is recommended to add protease inhibitors to the PBS) in a glass homogenizer and grind thoroughly on ice. To further lyse tissue cells, the homogenate can be sonicated or repeatedly frozen and thawed. Finally, centrifuge the homogenate at 5000×g for 5-10 minutes, and collect the supernatant for analysis. Cell Lysis Buffer: Adherent cells should be gently washed with pre-chilled PBS, then trypsinized and harvested by centrifugation at 1000×g for 5 minutes. Suspension cells can be harvested directly by centrifugation. Collected cells should be washed three times with pre-chilled PBS and resuspended in 150-200 μL of PBS per 1×10^6 cells (it is recommended to add protease inhibitors to the PBS; if the cell count is very low, reduce the PBS volume appropriately). Disrupt the cells by repeated freezing and thawing or sonication. Centrifuge the extract at 1500×g for 10 minutes at 2-8°C, and collect the supernatant for analysis. Other biological fluids: Centrifuge at 1000xg for 20 minutes, remove the supernatant, and test. Pre-test preparation: 1. Remove the test kit from the refrigerator 10 minutes in advance and equilibrate to room temperature. 2. Prepare the standard gradient working solution: Add 1 mL of universal diluent to the lyophilized standard, let it stand for 15 minutes to completely dissolve, then gently mix (concentration is 20 ng/mL). Then dilute to the following concentrations: 20 ng/mL, 10 ng/mL, 5 ng/mL, 2.5 ng/mL, 1.25 ng/mL, 0.625 ng/mL, 0.3125 ng/mL, and 0 ng/mL. Serial dilution method: Take 7 EP tubes and add 500 μL of universal diluent to each tube. Pipette 500 μL of the 20 ng/mL standard working solution into the first EP tube and mix thoroughly to make a 10 ng/mL standard working solution. Repeat this procedure for subsequent tubes. The last tube serves directly as a blank well; there is no need to aspirate the liquid from the penultimate tube. See the figure below for details. 3. Preparation of Biotinylated Antibody Working Solution: 15 minutes before use, centrifuge the concentrated biotinylated antibody at 1000×g for 1 minute. Dilute the 100× concentrated biotinylated antibody to a 1× working concentration using universal diluent (e.g., 10µL concentrate + 990µL universal diluent). Prepare immediately before use. 4. Prepare the enzyme conjugate working solution: 15 minutes before use, centrifuge the 100× concentrated enzyme conjugate at 1000×g for 1 minute. Dilute the 100× concentrated HRP enzyme conjugate to a 1× working concentration with universal diluent (e.g., 10 μL of concentrate + 990 μL of universal diluent). Prepare immediately. 5. Prepare the 1× wash solution: Dispense 10 mL of 20× wash solution into 190 mL of distilled water (concentrated wash solution removed from the refrigerator may crystallize; this is normal. Allow to stand at room temperature until the crystals have completely dissolved before preparing). Procedure: 1. Remove the desired strips from the aluminum foil bag after equilibration at room temperature for 10 minutes. Seal the remaining strips in a ziplock bag and return to 4°C. 2. Sample addition: Add 100 μL of sample or standard of varying concentrations to the corresponding wells. Add 100 μL of universal diluent to the blank wells. Cover with a film and incubate at 37°C for 60 minutes. (Recommendation: Dilute the sample to be tested at least 1-fold with universal diluent before adding it to the ELISA plate. This will reduce the impact of matrix effects on the test results. The sample concentration should be multiplied by the corresponding dilution factor when calculating the final sample concentration. It is recommended to run replicates for all test samples and standards.) 3. Add Biotinylated Antibody: Remove the ELISA plate and discard the liquid without washing. Add 100 μL of Biotinylated Antibody Working Solution directly to each well. Cover with a film and incubate at 37°C for 60 minutes. 4. Wash: Discard the liquid and add 300 μL of 1x Wash Solution to each well. Let stand for 1 minute, shake off the wash solution, and pat dry on absorbent paper. Repeat this process three times (a plate washer can also be used). 5. Add Enzyme Conjugate Working Solution: Add 100 μL of Enzyme Conjugate Working Solution to each well. Cover with a film and incubate at 37°C for 30 minutes. 6. Washing: Discard the liquid and wash the plate five times as in step 4. 7. Adding substrate: Add 90 μL of substrate (TMB) to each well, cover with a sealing film, and incubate at 37°C in the dark for 15 minutes. 8. Adding stop solution: Remove the ELISA plate and add 50 μL of stop solution directly to each well. Immediately measure the OD value of each well at a wavelength of 450 nm. Calculating experimental results: 1. Calculate the average OD value of the standard and sample replicates and subtract the OD value of the blank well as a correction factor. Plot the standard curve of the four-parameter logistic function on double-logarithmic graph paper, with concentration as the horizontal axis and OD value as the vertical axis. 2. If the sample OD value is higher than the upper limit of the standard curve, dilute the sample appropriately and retest. Multiply the sample concentration by the corresponding dilution factor. |
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| Theory | This kit uses a double-antibody sandwich enzyme-linked immunosorbent assay (ELISA). Sample, standard, biotin-labeled detection antibody, and HRP conjugate are sequentially added to microwells pre-coated with an interferon-induced protein with tetratricopeptide repeats 2 (IFIT2) capture antibody. After incubation and washing, the sample is developed using the substrate TMB. TMB converts to blue under the catalysis of HRP and to yellow under the action of acid. The intensity of the color is positively correlated with the amount of interferon-induced protein with tetratricopeptide repeats 2 (IFIT2) in the sample. The absorbance (OD) is measured at 450 nm using a microplate reader to calculate the sample concentration. | |||||||||||||||||||||||||||||||||
| Source | Human | |||||||||||||||||||||||||||||||||
| Synonym | Human Interferon-induced protein with tetratricopeptide repeats 2 | |||||||||||||||||||||||||||||||||
| Detection Type | Double antibody sandwich method | |||||||||||||||||||||||||||||||||
| Composition |
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| Background | Interferon-induced protein 2 (IFIT2) is a protein encoded by the IFIT2 gene. It may play a role in preventing tumor progression. Indeed, IFIT2 expression has been detected in human papillomavirus-positive keratinocytes from cervical precancerous lesions, where it is gradually downregulated at different malignant stages. Therefore, this gene may be involved in tumorigenesis and may serve as a potential prognostic marker for the progression of cervical precancerous lesions. | |||||||||||||||||||||||||||||||||
| General Notes | 1. Strictly adhere to the specified incubation time and temperature to ensure accurate results. All reagents must be at room temperature (20-25°C) before use. Refrigerate reagents immediately after use. 2. Improper plate washing may result in inaccurate results. Ensure that all liquid in the wells is aspirated thoroughly before adding substrate. Do not allow the wells to dry out during incubation. 3. Remove any residual liquid and fingerprints from the bottom of the plate, as this will affect the OD value. 4. The substrate developer solution should be colorless or very light in color. Do not use substrate solution that has turned blue. 5. Avoid cross-contamination of reagents and specimens to prevent erroneous results. 6. Avoid direct exposure to strong light during storage and incubation. 7. Do not expose any reagents to bleaching solvents or the strong fumes emitted by bleaching solvents. Any bleaching agent will destroy the biological activity of the reagents in the kit. 8. Do not use expired products, and do not mix components with different product numbers and batches. 9. Recombinant proteins from sources other than the kit may not be compatible with the antibodies in this kit and will not be recognized. 10. If there is a possibility of disease transmission, all samples should be managed properly and samples and testing devices should be handled according to prescribed procedures. |
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| Storage Temp. | If the unopened kit is stored at 4°C, the shelf life is 6 months. | |||||||||||||||||||||||||||||||||
| Test Range | 0.31-20 ng/mL | |||||||||||||||||||||||||||||||||
| Applications | Tissue homogenates, cell lysates, and other biological fluids |
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4.6 ★★★★★
Based on 18 reviews
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Product Reviews
★★★★★ 5
Magsafe car mount for a Tesla screen that is easy to adjust and holds the phone very well
Style: MagSafe Car Phone Holder, Style: MagSafe Car Phone Holder
I installed this mount on my 2018 Tesla Model 3 main display and it was easy to install - just loosen the wheels on the top and side clamp, put it in place and then tighten the clamps back down. There is a ball mount on the back which tightens as well that allows you to position and angle the phone mount so you can tilt it up or down or towards the driver. You can adjust the phone on the mount to where you want it - although the bar that holds the phone will hit the clamps in two spots so there are a couple of positions that don't work due to the design, but overall you can more or less put the phone where you want and and then tilt it any which way to you.
The magnet connection is very firm but the phone also detaches relatively easily. The clamps so far are holding well on the screen. I really don't have a lot of complaints - except maybe that there's a couple of positions that don't work. It works well, installs quickly and easily and seems to hold well in use. I've had no problems so ar.
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Reviewed in the United States on October 25, 2025
★★★★★ 4
Mostly reliable to use
Style: MagSafe Car Phone Holder
It's nice to use in my car to separate out different needs like looking up a specific route while moving and the navigation is going somewhere. The magnet is pretty strong and haven't had any issues with that. However, if you dont put it in the corner of the screen to secure it at a right angle, the flat way to secure it in the middle of the top edge is not reliable at all. The weight of the phone and the angle of the mount makes it really easy to fall off the screen no matter how tight you secure it down. Fell at least a handful of times. Just put it at the corners and you'll be just fine.
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Reviewed in the United States on December 29, 2025
★★★★★ 2
Does not fit the Cybertruck. Will slip off due to angled backplate.
Style: MagSafe Car Phone Holder, Style: MagSafe Car Phone Holder
It does not fit the Cybertruck well. Due to the angled monitor back, it will slide off. Check the video of what I mean. It is not flat like the Model 3 or Ys.
The mount should work fine on the Model 3 and Model Y and Newer Model S and X, but not on the Cybertruck and older vertical screen Model S and X.
The Magsafe that I received must of been broken. It completely fell apart. Everytime I put on phone on it, the Magsafe will completely come off stuck to my phone.
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Reviewed in the United States on October 30, 2025
★★★★★ 3
Looks good, installs easily but the arm jitters on the road
Style: MagSafe Car Phone Holder, Style: MagSafe Car Phone Holder
Super easy and intuitive to install on my Tesla model 3. Once installed, I think it will probably hold position indefinitely. You can install it so that it is in a very convenient position. my right hand index finger was able to touch the screen of my phone while maintaining contact with the handle, so that should give you an idea. The reason I took 2 stars off is the way the goose neck jitters when driving. I think perhaps it is too thin. I drove on normal paved road, and the shakes were noticeable. It didn’t fall off at any point, but it made me nervous that it might. The magnet didn’t feel that strong either. A lot of people own phones larger and heavier than mine (iPhone 12 mini), so I’m reluctant to recommend.
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Reviewed in the United States on October 26, 2025
★★★★★ 5
Well designed, secure hold
Style: MagSafe Car Phone Holder, Style: MagSafe Car Phone Holder
This Tesla screen phone mount feels well designed and has a strong MagSafe magnet to hold your phone. It is meant for the newer generation of Teslas with the screen that sits out from the instrument panel. It’s sits on the corner and has padding to keep it from scratching the screen. The size of the grippers keeps them right at the edge of the bevel so it doesn’t block any portion of the screen. They are easily tightened with a rotating dial that doesn’t slip. The MagSafe magnet can be positioned in a variety of positions. The kit comes with an adhesive metal ring in case your phone or case isn’t MagSafe compatible. Unfortunately, it didn’t come with any instructions, but it was easy enough to figure out.
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Reviewed in the United States on October 23, 2025