Human IL-17E/IL-25 ELISA Kit

Detection Principle:

This kit uses double antibody sandwich ELISA technology. The specific anti human il-17e capture antibody was pre coated on a high affinity microplate. Add the standard, the sample to be tested and the biotin labeled detection antibody into the wells of the enzyme plate in turn, shake well and mix well, and then place it at room temperature for 2 hours of incubation process. The il-17e in the sample is combined with the solid-phase antibody and the detection antibody. After washing sufficiently to remove free and unbound components, streptavidin HRP (sa-hrp) labeled with horseradish peroxidase was added. After washing again, TMB chromogenic substrate was added and incubated at room temperature in the dark to develop color. The depth of color response is positively correlated with the concentration of il-17e in the sample. Add stop solution to stop the reaction, and use a microplate reader to measure the absorbance value at 450 nm detection wavelength (correction wavelength 570-630 nm)

Detection Type: Double antibody sandwich method

Form: Pre coated 96 well plate

Test Sample Type: Cell supernatant, serum, plasma

Loading Amount: 100 μ L

Kit Components: a copy of pre coated 96 well plate, standard, il-17e detection antibody, standard dilution, detection buffer, TMB chromogenic substrate, washing solution, termination solution, sa-hrp, plate sealing membrane and instructions

Sensitivity: 2.39 pg/ml

Detection Range: 93.75-6000pg/ml

Recovery Range: 80-116%

Storage Method: 2-8 ℃

Standard Curve:

Background:

Interleukin 17e (il-17e), also known as IL-25, is a member of the IL-17 family and consists of type 2 helper T cells (Th2)And mast cell secretion production. It is similar to the sequence of IL-17 and can induce NF - & kappa; B activation, which stimulates the production of IL-8. Il-17e and IL-17B are ligands for the cytokine receptor IL-17RB. Studies in mice have shown that il-17e may be a pro-inflammatory cytokine that is beneficial to Th2 type immune response. Il-17e induces the production of other cytokines in a variety of tissues, including IL-4, IL-5, and IL-13, thereby stimulating eosinophilia. This cytokine is an important molecule that regulates intestinal immunity and participates in chronic inflammation associated with the gastrointestinal tract. Il-17e can kill some types of breast cancer cells.